Proceedings of The 1st International Electronic Conference on Entomology最新文献_第3页

piRNA Function in Insect Oogenesis piRNA在昆虫卵发生中的作用

Proceedings of The 1st International Electronic Conference on Entomology Pub Date : 2021-07-02 DOI: 10.3390/iece-10574

M. Piulachs, Andrea Lancho-Exposito, Oscar Paniello, J. Bau

{"title":"piRNA Function in Insect Oogenesis","authors":"M. Piulachs, Andrea Lancho-Exposito, Oscar Paniello, J. Bau","doi":"10.3390/iece-10574","DOIUrl":"https://doi.org/10.3390/iece-10574","url":null,"abstract":": Piwi-interacting RNAs (piRNAs) is a class of small non-coding RNAs (sncRNAs) of 28-29 nucleotides in length, poorly conserved across species. The first function associated with the piR-NAs was the repression of transposable elements (TE) expression. But, since that time, more piRNA functions have been discovered. They are not only repressors of TEs but also act as regulators of the expression of some mRNAs. A better understanding of the role of piRNAs is required and we focused our attention on their role in insect oogenesis. Using the cockroach Blattella germanica as experimental model, we studied the expression pattern of a piRNA specific to this cockroach (Bger_piRNA-40669). We analyzed the Bger_piRNA-40669 expression in adult ovaries during the first gonadotropic cycle, and to further study its function, we upregulated its expression using a chemically synthesized piRNA 2’ -O-methylated at the 3' end and phosphorylated at the 5' end. We found that an increase of Bger_piRNA-40669 in young adult females determines a shortening of the gonadotropic cycle, although embryogenesis was not affected. Moreover, our results show that Bger_piRNA-40669 has a specific function regarding oocyte development in B","PeriodicalId":109312,"journal":{"name":"Proceedings of The 1st International Electronic Conference on Entomology","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121071913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Simple Sequence Repeats (SSRs) Dynamics in Reticulitermes chinensis 中国白蚁简单序列重复序列(SSRs)动态分析

Proceedings of The 1st International Electronic Conference on Entomology Pub Date : 2021-07-02 DOI: 10.3390/iece-10566

Dr. Haroon, Yu-Xin Li, Chenxu Ye, Xiao-qin Ma, Jianming Su, X. Su, L. Xing

{"title":"Analysis of Simple Sequence Repeats (SSRs) Dynamics in Reticulitermes chinensis","authors":"Dr. Haroon, Yu-Xin Li, Chenxu Ye, Xiao-qin Ma, Jianming Su, X. Su, L. Xing","doi":"10.3390/iece-10566","DOIUrl":"https://doi.org/10.3390/iece-10566","url":null,"abstract":": An abundance of potential inherent variation in SSR or microsatellite repeats has resulted in a valuable genetic marker in eukaryotes. We describe how SSRs in Reticulitermes chinensis castes are organized and abundant. Collectively we sequence 184,436 unigenes through the Trinity system, ranging from 201-43, 214 bp length from transcriptome data. Using MISA to find out SSRs in uni-genes and a total of 10740 SSRs loci were identified as di-, tri-, tetra-, penta-, and hexanucleotide. Among them, trinucleotide SSRs were the most abundant in R. chinensis genome 2702, followed by chromosome six (tri-) 1110. Pentanucleotide repeats were found at frequency 1 from each chromosome 8, 9 and 13, respectively. The frequency of AC/GT motif was (21.91%) reported, followed by others motif (16.6%), AAG/CTT (8.49%) and AGC/CTG (8.2%). The minimum motif types were AATG/ATTC (1.27%), followed by ACG/CGT (1.32%) and AAT/ATT (1.77%). Thes abundance and inherent variations in SSRs provide valuable information for taxonomics, phylogenetic, genome mapping, and population genetic research studies. SSR-based markers have high degrees of allelic variability and codominant legacy ( inheritance), and analytical ease.","PeriodicalId":109312,"journal":{"name":"Proceedings of The 1st International Electronic Conference on Entomology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129277952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Combined Administration of Entomopathogenic Bacteria to Drosophila suzukii Larvae 虫媒致病菌联合施用对苏氏果蝇幼虫的影响

Proceedings of The 1st International Electronic Conference on Entomology Pub Date : 2021-07-02 DOI: 10.3390/IECE-10578

M. Brivio, M. Mastore, Sara Caramella

{"title":"Effects of Combined Administration of Entomopathogenic Bacteria to Drosophila suzukii Larvae","authors":"M. Brivio, M. Mastore, Sara Caramella","doi":"10.3390/IECE-10578","DOIUrl":"https://doi.org/10.3390/IECE-10578","url":null,"abstract":"Many invasive insect species that have moved from eastern areas, among them Drosophila suzukii Matsumura (Diptera: Drosophilidae), also named spotted wings drosophila (SWD), is becoming a serious threat to the cultivation and marketing of thin-skinned red fruits. The SWD, endemic to Southeast Asia, have been introduced in North America and Europe a decade ago. The control of the diffusion of SWD, is carried out with various methods, based on different strategies, but the use of pesticides is still extremely common. Possible alternatives to chemical pesticides are biological control methods based on bioinsecticides. Up to now, biological control has been performed mainly through the application of single bioinsecticides, but the major limitation is that they produce appreciable results only in quite long time, so it would be desirable to obtain a significant mortality in the shortest time of treatment; the use of targeted combinations of more bioinsecticides, could represent an improvement in the control techniques of D. suzukii.In this research we carried out a preliminary investigation on the effects of administration of Bacillus thuringiensis (Bt) with Xenorhabdus nematophila (Xn) isolated from Steinernema carpocapsae, or with Xn secretions, on D. suzukii early instars larvae. Firstly, we assayed the lethal effects of individual bioinsecticides administration, then we evaluated a possible increase in efficacy induced by their combination. Moreover, we have analyzed the morphology of the host gut to evaluate a possible damage caused by combination Bt-Xn after oral uptake. From the results obtained in the laboratory, it emerges that biological control techniques carried out by means of bioinsecticide combinations, can improve the action, therefore the effectiveness, of these methods, which could subsequently be tested and applied in the field.","PeriodicalId":109312,"journal":{"name":"Proceedings of The 1st International Electronic Conference on Entomology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133268394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Barcoding of 15 Species of African Saturniidae (Lepidoptera) across 12 Genera Reveals High Incidence of Non-Monophyly 非洲鳞翅目12属15种的DNA条形码显示非单系发生率高

Proceedings of The 1st International Electronic Conference on Entomology Pub Date : 2021-07-02 DOI: 10.3390/iece-10573

Zwannda Nethavhani, Kayleigh Hiscock, B. van Asch

引用次数: 0

Identification of Small-Molecule Potential Inhibitor(s) for Helicoverpa armigera Juvenile Hormone Acid-o-Methyl Transferase (HaJHAMT) through Molecular Docking and MD Simulation Approaches 利用分子对接和MD模拟方法鉴定棉铃虫幼年激素酸-邻甲基转移酶(HaJHAMT)小分子潜在抑制剂

Proceedings of The 1st International Electronic Conference on Entomology Pub Date : 2021-07-02 DOI: 10.3390/iece-10570

Ashwani Sharma, Rakesh Kumar, Joyashri Das, Monica Sharma, Pravindra Kumar

{"title":"Identification of Small-Molecule Potential Inhibitor(s) for Helicoverpa armigera Juvenile Hormone Acid-o-Methyl Transferase (HaJHAMT) through Molecular Docking and MD Simulation Approaches","authors":"Ashwani Sharma, Rakesh Kumar, Joyashri Das, Monica Sharma, Pravindra Kumar","doi":"10.3390/iece-10570","DOIUrl":"https://doi.org/10.3390/iece-10570","url":null,"abstract":": Juvenile hormone compounds belong to a family of acyclic sesquiterpenoids, biosynthe-sized within small paired endocrine glands of corpora allata-corpora cardiaca having a neural connection with the brain. Stringent regulation of JH-levels in insect haemolymph is one of the most critical factors ensuring normal growth and development in insects. JHAMT is characterized as a key regulatory enzyme playing a pivotal role during insect metamorphosis and reproduction physiology by cat-alyzing the final step of SAM-dependent methylation of JH-acids into their cognate methyl esters. Recent reports on biochemical and molecular characterization of JHAMT gene from different insect species have proposed the JHAMT enzymatic activity as a potential target for the development of novel small molecule insect growth regulator(s). Therefore, in the present study, the protein sequence identity dependent homology model for JHAMT protein from a polyphagous pest, Helicoverpa armigera (order: Lepidoptera) has been generated and subsequently explored for virtual screening against small molecules natural product library. One such promising compouns namely, Scolimoside (HMDB05799) showing interactions with amino acid residues of enzyme's substrate-binding pocket (SBP) was further validated using molecular docking and Molecular Dynamics simulation (MD simulation). The study may pave the way forward for the design and development of novel eco-friendly small regulatory molecules as a component of integrated pest management.","PeriodicalId":109312,"journal":{"name":"Proceedings of The 1st International Electronic Conference on Entomology","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122727042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sage Essential Oil Composition from Kabylia (Algeria) and Its Biological Effects Against Cowpea Weevil (Callosobruchus maculatus) (Coleoptera: Bruchidae) 阿尔及利亚卡比利亚鼠尾草挥发油成分及其对豇豆象鼻虫的生物防治作用(鞘翅目:野象科)

Proceedings of The 1st International Electronic Conference on Entomology Pub Date : 2021-07-02 DOI: 10.3390/iece-10575

Karima Taleb-Toudert, A. Kellouche

引用次数: 0

Probing Carotenoids in the Gall Wasp Aulacidea hieracii in Vivo 在瘿蜂体内探测类胡萝卜素

Proceedings of The 1st International Electronic Conference on Entomology Pub Date : 2021-07-02 DOI: 10.3390/iece-10565

E. Nikelshparg, D. Bratashov, M. Nikelshparg, V. Anikin

引用次数: 0

A Comparison of Ground Beetle Assemblages (Coleoptera: Carabidae) between Birch Forests of Continuous and Isolated Peat Bog Habitats in Belarusian Lake District 白俄罗斯湖区连续与孤立泥炭沼泽桦林地面甲虫群落比较(鞘翅目:甲虫科)

Proceedings of The 1st International Electronic Conference on Entomology Pub Date : 2021-07-02 DOI: 10.3390/iece-10564

G. Sushko

{"title":"A Comparison of Ground Beetle Assemblages (Coleoptera: Carabidae) between Birch Forests of Continuous and Isolated Peat Bog Habitats in Belarusian Lake District","authors":"G. Sushko","doi":"10.3390/iece-10564","DOIUrl":"https://doi.org/10.3390/iece-10564","url":null,"abstract":": Largest in Central Europe ancient and almost intact Belarusian peat bogs are habitats with very specific environmental conditions and biodiversity. They themselves are island-like ecosystems, but in addition they are containing small raised islands of mineral soil within peat, covered by forest. In this study, the ground beetle diversity, species composition and abundance according to their habitat affinity, mean body size and wing form between isolated birch forests on the mineral and peat soils within the peat bog and adjacent continuous birch forests were compared. The diversity respond to isolation most clearly in birch forests with sphagnum cover, while in forests on the mineral soils the diversity parameters were similar to continuous adjacent forests. Distinct separation of carabid assemblages of continuous and isolated habitats was detected only in terms of species composition. There is a clear increase of the abundances of forest species from continuous to isolated forests on mineral soil, whereas in the forests on peat soil specialized peat bog species were dominated. In isolated forests, a gradually decrease of the abundances of large-sized and brachypterous species and increase of medium-sized beetles were recorded. However, the abundance of macropterous species was the most abundant only in isolated forest on mineral soil. Thus, the ground beetle assemblages of the two birch forest types within peat bog respond in a different way to the isolation and recorded differences can be probably explained also a variety of environmental factors.","PeriodicalId":109312,"journal":{"name":"Proceedings of The 1st International Electronic Conference on Entomology","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121110919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards the Development of a Smart Plant Protection Solution for Improved Pest Management of Dry Beans (Phaseolus vulgaris L.) in Northern Greece 希腊北部干豆(Phaseolus vulgaris L.)病虫害管理智能植物保护解决方案的开发

Proceedings of The 1st International Electronic Conference on Entomology Pub Date : 2021-07-02 DOI: 10.3390/iece-10568

P. Damos, Evaggelos Tsikos, M. Louta, F. Papathanasiou

引用次数: 2

Hunting Nematodes in the Pine Forests of Northern Greece: A Preliminary Overview after One Year of Surveys 在希腊北部松林中狩猎线虫:经过一年的调查后的初步概述

Proceedings of The 1st International Electronic Conference on Entomology Pub Date : 2021-07-01 DOI: 10.3390/iece-10526

Maria Karmezi, Alkmini Bataka, D. Papachristos, D. Avtzis

引用次数: 0