CSH protocols最新文献_第7页

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 1: Coating Glass Slides with Amino Silane. 核酸可编程蛋白阵列(NAPPA)的构建1:氨基硅烷涂覆玻片。

CSH protocols Pub Date : 2008-11-01 DOI: 10.1101/pdb.prot5056

Andrew J Link, Joshua Labaer

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引用次数: 9

Isolation, culture, and differentiation of progenitor cells from the central nervous system. 中枢神经系统祖细胞的分离、培养和分化。

CSH protocols Pub Date : 2008-11-01 DOI: 10.1101/pdb.prot5077

Scott R Hutton, Larysa H Pevny

引用次数: 29

Tomato (Solanum lycopersicum): A Model Fruit-Bearing Crop. 番茄(Solanum lycopersicum):一种典型的果实作物。

CSH protocols Pub Date : 2008-11-01 DOI: 10.1101/pdb.emo105

Seisuke Kimura, Neelima Sinha

{"title":"Tomato (Solanum lycopersicum): A Model Fruit-Bearing Crop.","authors":"Seisuke Kimura, Neelima Sinha","doi":"10.1101/pdb.emo105","DOIUrl":"https://doi.org/10.1101/pdb.emo105","url":null,"abstract":"INTRODUCTIONTomato (Solanum lycopersicum) is one of the most important vegetable plants in the world. It originated in western South America, and domestication is thought to have occurred in Central America. Because of its importance as food, tomato has been bred to improve productivity, fruit quality, and resistance to biotic and abiotic stresses. Tomato has been widely used not only as food, but also as research material. The tomato plant has many interesting features such as fleshy fruit, a sympodial shoot, and compound leaves, which other model plants (e.g., rice and Arabidopsis) do not have. Most of these traits are agronomically important and cannot be studied using other model plant systems. There are 13 recognized wild tomato species that display a great variety of phenotypes and can be crossed with the cultivated tomato. These wild tomatoes are important for breeding, as sources of desirable traits, and for evolutionary studies. Current progress on the tomato genome sequencing project has generated useful information to help in the study of tomato. In addition, the tomato belongs to the extremely large family Solanaceae and is closely related to many commercially important plants such as potato, eggplant, peppers, tobacco, and petunias. Knowledge obtained from studies conducted on tomato can be easily applied to these plants, which makes tomato important research material. Because of these facts, tomato serves as a model organism for the family Solanaceae and, specifically, for fleshy-fruited plants.","PeriodicalId":10835,"journal":{"name":"CSH protocols","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1101/pdb.emo105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29702120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 173

Ctenophore tissue preparation and extraction of DNA. 栉水母组织的制备及DNA的提取。

CSH protocols Pub Date : 2008-11-01 DOI: 10.1101/pdb.prot5088

Kevin Pang, Mark Q Martindale

{"title":"Ctenophore tissue preparation and extraction of DNA.","authors":"Kevin Pang, Mark Q Martindale","doi":"10.1101/pdb.prot5088","DOIUrl":"https://doi.org/10.1101/pdb.prot5088","url":null,"abstract":"INTRODUCTIONCtenophores, or comb jellies, are a group of marine animals whose unique biological features and phylogenetic placement make them a key taxon for understanding animal evolution. Some characteristics are present in nearly all ctenophores, including biradial symmetry, comb rows composed of linked cilia, an apical sensory organ, and two tentacles bearing specialized adhesive cells. All ctenophores studied thus far have the same stereotyped cleavage program and go through a specific stage of development known as the cydippid larva, after which adult structures develop and diverge greatly among species; this is particularly useful for comparative studies. In some cases, gene expression patterns appear to be conserved. Of particular interest is the finding that some genes are expressed in regions of the ctenophore body that are not morphologically distinct from the adjacent areas. However, it has proven difficult to determine the orthology of some genes, possibly because of the extreme divergence of ctenophore representatives. This protocol describes how to isolate genomic DNA from ctenophores. The procedure can be applied to adult tissues, but it is best to use embryos and larvae. After washing and concentrating the embryos or larvae, DNA is extracted using DNAzol reagent, a guanidine-detergent lysing solution. The resulting DNA can be used for polymerase chain reaction (PCR) or other applications.","PeriodicalId":10835,"journal":{"name":"CSH protocols","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1101/pdb.prot5088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29701402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 5: Expressing Proteins on NAPPA Slides. 核酸可编程蛋白阵列(NAPPA)的构建5:在NAPPA载玻片上表达蛋白。

CSH protocols Pub Date : 2008-11-01 DOI: 10.1101/pdb.prot5060

Andrew J Link, Joshua Labaer

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引用次数: 2

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 6: Detecting Proteins on NAPPA Slides. 核酸可编程蛋白阵列(NAPPA)的构建6:在NAPPA载玻片上检测蛋白质。

CSH protocols Pub Date : 2008-11-01 DOI: 10.1101/pdb.prot5061

Andrew J Link, Joshua Labaer

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引用次数: 4

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 7: Detecting DNA on NAPPA Slides. 核酸可编程蛋白阵列(NAPPA)的构建7:在NAPPA载玻片上检测DNA。

CSH protocols Pub Date : 2008-11-01 DOI: 10.1101/pdb.prot5062

Andrew J Link, Joshua Labaer

{"title":"Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 7: Detecting DNA on NAPPA Slides.","authors":"Andrew J Link, Joshua Labaer","doi":"10.1101/pdb.prot5062","DOIUrl":"https://doi.org/10.1101/pdb.prot5062","url":null,"abstract":"INTRODUCTIONFunctional proteomics enables protein activities to be studied in vitro using high-throughput (HT) methods. Protein microarrays are the method of choice because they display many proteins simultaneously and require only small reaction volumes to assess function. Protein microarrays are typically used to (1) measure the abundance of many different analytes in a sample or (2) study the functions or properties of many proteins spotted on the array. Target protein microarrays are usually generated by expressing, purifying, and spotting the proteins onto a solid surface at very close spatial density. An alternative approach is to translate the proteins in situ on the array surface. This method uses cell-free extracts that transcribe and translate DNA into proteins which are then captured in situ, thus converting cDNA copies of genes into the desired target proteins. Instead of printing proteins at each feature of the array, the cDNA molecules for the corresponding genes that produce desired proteins are affixed to the array. Chemical treatment of glass slides and DNA isolation can be performed in advance and stored. The plasmid DNA can then be printed to make NAPPA slides, which can be stored dry for use. For experiments, NAPPA slides are expressed followed by detection of proteins and DNA using antibodies and stains. This protocol describes detection of DNA on arrayed slides to assess the amount of DNA captured.","PeriodicalId":10835,"journal":{"name":"CSH protocols","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1101/pdb.prot5062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29702074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Grafting tomato plants. 嫁接番茄植株。

CSH protocols Pub Date : 2008-11-01 DOI: 10.1101/pdb.prot5083

Seisuke Kimura, Neelima Sinha

引用次数: 3

Determining the Sex of Adult Astyanax mexicanus. 测定墨西哥Astyanax成虫的性别。

CSH protocols Pub Date : 2008-11-01 DOI: 10.1101/pdb.prot5090

Richard Borowsky

引用次数: 4

In Vitro Fertilization of Astyanax mexicanus. 墨菖蒲的体外受精研究。

CSH protocols Pub Date : 2008-11-01 DOI: 10.1101/pdb.prot5092

Richard Borowsky

引用次数: 8