Current Analytical Chemistry最新文献

{"title":"Optimization of Pseudotargeted Metabolomics: Fully Integrating the Advantages of Both Targeted and Untargeted Approaches","authors":"Yong Li, Pang Tao, Junli Shi, Zhenmin Xu, He Xie, Ge Bai, Xuejun Chen, Lu Zhao","doi":"10.2174/0115734110392891250831141601","DOIUrl":"https://doi.org/10.2174/0115734110392891250831141601","url":null,"abstract":"Introduction: This study aimed to address persistent challenges in pseudotargeted metabolomics, particularly the limited compatibility with diverse sample types, by developing an enhanced method integrating the strengths of targeted and untargeted approaches. Methods: An upgraded pseudotargeted metabolomics method was developed, incorporating a sample- specific MS-RI library (SSMSRIL) to identify novel metabolites in new samples. Newly discovered metabolites were dynamically added to a pseudotargeted MRM list. Additionally, MRM transitions for 227 target metabolites were integrated, resulting in a final method monitoring >500 metabolites. This design facilitates the extraction and addition of new metabolites to the monitoring list. The method was established and evaluated using gas chromatography-tandem mass spectrometry (GCMS/ MS). Results: Evaluation with new samples revealed that 33-40% of all detected metabolites were identified exclusively through the integrated targeted MRM transitions. This demonstrated their significant role in expanding metabolite coverage. Furthermore, 23-54% of metabolites detected in new sample types were absent from the initial SSMSRIL list. Discussion: The substantial proportion (23-54%) of metabolites detected in new sample types missing from the original library underscores the critical necessity of dynamically updating the pseudotargeted MRM list when applying the method to new samples. This update mechanism is vital for maintaining broad metabolite coverage and method applicability across diverse sample matrices. result: The new method monitors over 500 metabolites, allowing for easy extraction and addition of new metabolites to the monitoring list. Evaluation with new samples revealed that 33-40% of all detected metabolites were identified solely through the targeted MRM transitions, indicating a significant expansion of metabolite coverage. Furthermore, 23-54% of detected metabolites from new sample types were not in the initial SSMSRIL list, highlighting the importance of updating the pseudotargeted MRM list for different sample types. Conclusion: The enhanced pseudotargeted method significantly improves metabolite coverage and adaptability to new sample types through dynamic MRM list updating and the integration of targeted MRM transitions. While developed using GC-MS/MS, the core concept is readily transferable to liquid chromatography (LC)-based full-scan and MRM methodologies, broadening its potential impact.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147896374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogels Doped with Inorganic Ammonium Chloride Salt for Fuel Cell Applications","authors":"Shihao Yang, C.G. Wang, Hao Wu, Xia Xiang, Xiaoyi Zeng, Pengwei Li","doi":"10.2174/0115734110385450250730112440","DOIUrl":"https://doi.org/10.2174/0115734110385450250730112440","url":null,"abstract":"Introduction: Inorganic ammonium salts, such as ammonium chloride (NH4Cl), can form electrolytes due to their protonated ammonium ions acting as proton carriers. This study investigates the use of NH4Cl-doped polyacrylamide hydrogel as a flexible proton conductor for fuel cell applications. Methods: A freestanding hydrogel membrane was fabricated through in situ polymerization of ethylene glycol dimethacrylate and acrylamide within aqueous NH4Cl. The hydrogel';s mechanical and conductive properties were characterized, and it was assembled into a fuel cell to evaluate its performance. Results: The NH4Cl-doped hydrogel exhibited a fracture tensile stress of 90 kPa at 462% elongation. The conductivity measurements, which varied with temperature, revealed that proton conduction primarily followed a vehicle mechanism, showcasing an impressive ionic conductivity of 119 mS cm?¹. The fuel cell achieved a maximum power density of 31.9 mW cm?², marking a solid performance. Discussion: The \"structure-performance\" relationship of inorganic ammonium salt-doped hydrogels is clarified through multi-scale characterization. The dual influence mechanism of NH4 + concentration on proton conduction has been revealed. The developed HGA-n material combines excellent mechanical strength and proton conductivity, and its power density provides a new option for gel electrolytes used in fuel cells. Conclusions: A flexible proton conductor is prepared by doping ammonium chloride (NH4Cl) into polyacrylamide hydrogel for the first time and is successfully assembled into a fuel cell. This work demonstrates a novel approach for utilizing inorganic ammonium salts in electrochemical applications, offering a promising route for developing flexible proton-conducting materials for fuel cells.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147909598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Key lncRNAs and ceRNA Regulatory Networks in Diabetes-Associated Cognitive Dysfunction","authors":"Yingying Deng, Juan Hu, Lan Yang, Shiqiu Jiang, Hailiang Du, Yansong Li, Yaomin Zhu","doi":"10.2174/0115734110374941250717134147","DOIUrl":"https://doi.org/10.2174/0115734110374941250717134147","url":null,"abstract":"Introduction: Diabetes clinical guidelines have begun to emphasize the management of cognitive dysfunction in diabetes. Moreover, several studies have shown that long non-coding RNAs (lncRNAs) play critical roles in human diseases. However, no studies have elucidated whether lncRNAs are involved in the pathogenesis of diabetes-associated cognitive dysfunction (DACD). Methods: The hippocampi of male mice with homozygous leptin receptor-deficient T2DM and their littermates were analyzed via high-throughput sequencing. RNA and protein sequencing data were utilized to identify differentially expressed (DE) mRNAs, lncRNAs, and proteins between diabetes mellitus and control groups. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed for DE-mRNAs-DE-proteins. Subsequently, lncRNA-mRNA-pathway and lncRNA-miRNA-mRNA networks were constructed. Quantitative real-time PCR (qRT-PCR) was conducted to verify the expression trends of key lncRNAs in DACD. Results: We identified upregulated and downregulated mRNAs, lncRNAs, and proteins in the diabetes group. Eleven DE-mRNAs-DE-proteins were associated with inflammatory response, lipid/ steroid metabolism, and cell growth. The lncRNA-mRNA-pathway network contained 36 lncRNA-mRNA pairs linked to 8 KEGG pathways. Twenty-five lncRNAs corresponding to Apoa1 were identified as key candidates. Subcellular localization and ceRNA network analyses suggested that lncRNA 3110039I08Rik and lncRNA Gm36445 may regulate DACD. qRT-PCR confirmed their increased expression in diabetic mice. Discussion: Our findings reveal that lncRNA 3110039108Rik and Gm36445 may act as critical regulators in DACD pathogenesis through competing endogenous RNA (ceRNA) networks. These lncRNAs represent potential biomarkers for early diagnosis and therapeutic targets, offering new insights into the management of cognitive dysfunction in diabetes. Conclusion:: Our results identified two novel lncRNAs that may play critical roles in DACD.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147916733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient Extraction Coupled with Liquid Chromatography for theAnalysis of Toxic Alkaloids in Wudang Taoist ink","authors":"Yongheng Zhao, Mei Zeng, Yongchang Jin, Zhixiong Jin, Ling Xu","doi":"10.2174/0115734110367259250610033446","DOIUrl":"https://doi.org/10.2174/0115734110367259250610033446","url":null,"abstract":"Background: The alkaloids present in Wudang Taoist ink are both potent and toxic. Thus, it is essential to assess their concentrations. In this study, mesaconitine, aconitine, and strychnine were used as indicators of safety. Methods: The extraction and quantification of these alkaloids were accomplished through ethanol heating reflux extraction and high-performance liquid chromatography (HPLC), utilizing Radix Aconiti Preparata, Radic Aconiti Kusnezoffii Preparata, and Processed Semen Strychni as raw materials. Guided by single-factor experiments, we employed response surface methodology to optimize the extraction process. The primary objective of this optimization was to maximize the extraction efficiency of total aconitine (which includes mesaconitine and aconitine) and strychnine, while considering key factors such as ethanol concentration, material-to-liquid ratio, and extraction time. Using the optimized extraction protocol, we successfully extracted and quantified mesaconitine, aconitine, and strychnine content in Wudang Taoist ink. Results: The optimal conditions included an ethanol concentration of 77.9%, a liquid-to-material ratio of 8.85:1 (mL/g), and an extraction time of 135 minutes. This approach demonstrated high accuracy and precision. Conclusion: The alkaloid content in the ink meets the requirements specified in the Chinese Pharmacopoeia. The established response surface methodology is both reliable and reproducible, making it applicable for controlling toxic components in Radix Aconiti as well as in Wudang Taoist ink. result: The optimal conditions used an ethanol concentration of 77.86% and a material-to-liquid ratio of 8.5:1 (mg/L), with an extraction time of 135 minutes. The approach achieved high accuracy and precision.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147887119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical Characterization, Hypoglycemic Activity, and Antioxidant Activity of the Ultrasonic-assisted Extracted Polysaccharides from Sanghuangporus sanghuang","authors":"Pingping Zhang, Jing Xu, Boya Zhao, Dongfang Shi, Yunhe Qu","doi":"10.2174/0115734110370023250509111927","DOIUrl":"https://doi.org/10.2174/0115734110370023250509111927","url":null,"abstract":"Introduction: Sanghuangporus sanghuang (S. sanghuang) is a precious macrofungus. Polysaccharides are the functional components in S. sanghuang and have a variety of biological activities. Ultrasonic-assisted extraction is an efficient method to extract polysaccharides. It is necessary to study the structure and biological activity of polysaccharides from S. sanghuang to make use of this macrofungal resource. Methods: The S. sanghuang polysaccharide (SSP) was extracted by ultrasonic-assisted extraction. The structure of SSP was characterized using monosaccharide composition and molecular weight distribution analysis, Ultraviolet-Visible spectrum (UV), Fourier Transform Infrared spectroscopy (FT-IR), Dynamic Light Scattering (DLS), X-Ray Diffractometer (XRD) and Scanning Electron Microscope (SEM). The hypoglycemic potential of SSP was assessed by measuring its ability to inhibit α-amylase and α-glucosidase. Additionally, the antioxidant capacity of SSP was evaluated using the Ferric Reducing Ability of Plasma (FRAP) assay, as well as its ability to scavenge ABTS+, DPPH, and OH free radicals. Results: The SSP was extracted by ultrasonic-assisted extraction, and the yield was 4.094±0.003%. Structural analysis revealed that SSP was an α-linked heteroglucan primarily composed of glucose. Its molecular weight distribution was uneven, mainly centered around 16.7 kDa and 5.1 kDa. SSP was an amorphous structure with a spherical surface and an average particle size of 129.17±5.12 nm in an aqueous solution. For α-amylase and α-glucosidase inhibition assay, IC50 values were 0.234±0.058 mg/mL and 1.173±1.009 mg/mL, respectively. The IC50 values for DPPH, ABTS+, and OH free radical scavenging assays were 0.047±0.009 mg/mL, 0.005±0.003 mg/mL, and 4.887±0.255 mg/mL, respectively. Therefore, SSP exhibited dose-dependent and strong α-amylase and α- glucosidase inhibition capacity, reducing power and radical scavenging activities. Discussion: These findings provide valuable insights for the development of functional polysaccharides with potential applications in nutraceuticals or therapeutic interventions for diabetes and oxidative stress-related disorders. Conclusion: In this study, an α-linked heteroglucan, known as SSP, demonstrated effective hypoglycemic activities and antioxidant capabilities, which were dependent on dosage. Our results contribute significantly to the development of polysaccharides possessing potential antioxidant and hypoglycemic qualities.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"22 5","pages":"918-928"},"PeriodicalIF":0.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148672227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RSM-rCCD Optimizing for Paclitaxel Extraction from Taxus chinensis byNatural Deep Eutectic Solvents and Studying Antioxidant Activity","authors":"Ying Guo, Izni Atikah Abd Hamid, Chan Mieow Kee, Jiaxin Quan","doi":"10.2174/0115734110367450250323104325","DOIUrl":"https://doi.org/10.2174/0115734110367450250323104325","url":null,"abstract":"Background: Paclitaxel from Taxus chinensis contains medicinal properties to treat various cancer diseases. Thus, extracting paclitaxel from Taxus chinensis had aroused a wide of research interests. Methods: Ultrasonic-assisted extraction (UAE) has high extraction efficiency, and natural deep eutectic solvents (NADESs) have the advantages of being green, natural, and non-toxic. As a result, the UAE-NADESs were introduced to extract paclitaxel from Taxus chinensis. The ideal NADES composed of choline chloride and malic acid was achieved at a molar ratio of 1:1, with the optimal extraction parameters identified through single-factor experiments. Response Surface Methodology of rotatable Central Composite Design (RSM-rCCD) was applied to further optimize the extraction conditions. Results: The ultimate optimum circumstances of ultrasonic power is 240 W, extraction time is 49 min, solid/liquid ratio is 1:18, extraction temperature is 38°C, and the maximum extraction yield reached 5.94 mg/g. Their IC50 values of paclitaxel extract for DPPH• (2,2-diphenyl-1- picrylhydrazyl), ABTS+• (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) and·•OH (Hydroxyl radical) radicals were 23.47, 46.62 and 309.73 mg/L, respectively. method: Ultrasonic-assisted extraction; single factor-experiments; response surface methodology; antioxidant activities of paclitaxel extract. Conclusion: This article demonstrates the increased extraction yield of paclitaxel, which has strong antioxidant activity.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Norfloxacin Adsorption and Detection in WastewaterUtilizing Hyaluronic Acid and Dopamine-modified Mesoporous Carbon","authors":"Yanhui Ge, Yubo Liu","doi":"10.2174/0115734110364328250121234405","DOIUrl":"https://doi.org/10.2174/0115734110364328250121234405","url":null,"abstract":"Objective: The increasing environmental pollution from antibiotics poses a significant threat to public health, and this is a critical issue that requires immediate attention. Methods: In this study, a simple and effective surface modification technique was presented using hyaluronic acid-dopamine conjugate (HA-DA) to impart anti-biofouling properties to basin-concave mesoporous carbon (BCMC). The synthesized materials were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, nitrogen physisorption, and thermogravimetric analysis (TGA). The optimum adsorption performance of the synthesized materials was investigated via adsorption isotherms and kinetics experiments. Afterward, the obtained particles were used as solid-phase extraction subjects for Norfloxacin (Nor) analysis in wastewater. Results: After being quantified by high-performance liquid chromatography (SPE-HPLC), the synthesized BCMC@HA-DA demonstrated an impressive binding capacity of 14.80 mg/g for Nor. Following six adsorption-desorption cycles, the adsorption revercory remained at 81.65%. Conclusion: The prepared BCMC@HA-DA could successfully be concentrated Nor from wastewater, indicating significant potential for addressing environmental biofouling issues.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"22 4","pages":"701-714"},"PeriodicalIF":0.0,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147913275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Lithium-containing Fly Ash Derived from Coal-fired Power Plants in Guizhou Province, China","authors":"Xiwei Mu, Longjiao Lv, Xiru Cao, Jingfeng Liu, Fayue Li, Chun‐Ying Liu, Yuting Ren, Cao Ban, Bozhi Ren, Shuai Xu, Zhangping Wu, Guocheng Zhu","doi":"10.2174/0115734110349242241206065556","DOIUrl":"https://doi.org/10.2174/0115734110349242241206065556","url":null,"abstract":"Background: Coal-fired power plants, such as those located in Guizhou, are generating substantial amounts of fly ash. This fly ash is rich in lithium, a highly valuable metal utilized in numerous industrial applications. Effective extraction of these metals necessitates a thorough understanding of their characteristics within the fly ash. Methods: Multiple analytical techniques were used to study fly ash';s physicochemical properties. Meanwhile, wet magnetic separation and acid-base methods are employed to separate its mineral phases. Results: The fly ash mainly contains mullite, glass, and iron microspheres, showing spherical particle morphology. It';s rich in O, Si, Al, Fe, C, and oxides like SiO2, Al2O3, Fe2O3. Most particles are smaller than 20 µm. Lithium in the ash existed as Li-O-M compounds, mainly in the non-magnetic and glass phases. Conclusion: Overall, these findings have deepened our understanding of fly ash and the distribution of lithium within it, providing a foundation for the recovery of lithium and other valuable elements.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147917718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive Electrochemical Sensing Properties for Cyanide Detection Using Polyaniline/Cu-Vanadate Nanobelt-modified Electrode","authors":"Y.D. Zhang, Qianmin Cong, Chenxu Feng, Zhengyu Cai, L. Z. Pei","doi":"10.2174/0115734110322886240918105204","DOIUrl":"https://doi.org/10.2174/0115734110322886240918105204","url":null,"abstract":"Background: Cyanide is a toxic anion and may be discarded into the water environment, which is a serious threat to human beings and the environment. Hence, it is essential to explore a facile, sensitive method for cyanide detection. Polyaniline/Cu vanadate nanobelts serve as electrode materials for sensitive cyanide determination. Methods: Polyaniline/Cu vanadate nanobelts were obtained by a simple route using polyaniline, Cu vanadate nanobelts. The polyaniline/Cu vanadate nanobelts were measured via electron microscopy, diffraction, infrared spectrum, and electrochemical methods. Results: Amorphous polyaniline nanoparticles with a particle size of about 100 nm were attached firmly to the surface of Cu vanadate nanobelts. Electrochemical sensing properties for cyanide detection were analyzed using a cyclic voltammetry technique using the nanobelts-modified electrode. The cyclic voltammograms (CV) peaks centered at +0.64 V and +0.54 V were observed using the polyaniline/Cu vanadate nanobelts in 0.1 M KCl solution with 2 mM cyanide. The proposed composite nanobelt-modified electrode possessed the detection range and detection limit of 0.001-2 mM and 0.22 μM, respectively. Conclusion: Polyaniline greatly enhances the electro-catalytic performance of the Cu vanadate nanobelt-modified electrode for cyanide detection.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"21 7","pages":"877-883"},"PeriodicalIF":0.0,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147886693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification and Kinetics of Chlorogenic Acid from Eucommia ulmoides Oliver Leaves by Macroporous Resins Combined with First-Principles Calculation","authors":"Zufei Feng, Yan Zhao, Sijin Li, Yangfan Hu, Yiming Zou, Yingjuan Zhao, Rong Yang, Yihui Yang, Na Wu","doi":"10.2174/0115734110313275240820100255","DOIUrl":"https://doi.org/10.2174/0115734110313275240820100255","url":null,"abstract":"Background: A simple and effective method to separate chlorogenic acid from Eucommia ulmoides leaves with macroporous resin was studied in this paper. Methods: In order to optimize the separation process of chlorogenic acid from Eucommia ulmoides leaves, dynamic adsorption and desorption experiments were carried out on a glass column filled with XDA-8 resin. Based on the First-principles calculation, the possible adsorption models were simulated. Results: Among the six macroporous resins, XDA-8 showed good adsorption/desorption capacity and a high adsorption/desorption ratio for chlorogenic acid. After being treated with XDA-8 resin once, the content of chlorogenic acid from the extraction increased by 525%, and the recovery of chlorogenic acid reached 85.36%. Conclusion: At 25°C, the adsorption behavior of chlorogenic acid on XDA-8 resin was consistent with the Langmuir isotherm model and pseudo-second-order kinetic model. Furthermore, by calculating the charge changes of the O atom at each position in the chlorogenic acid molecule and the H atom at the adsorption site in polystyrene molecule with resin skeleton, and combining with the electron cloud density distribution diagram of chlorogenic acid and resin skeleton, the adsorption of chlorogenic acid by XDA-8 resin is mainly due to the charge transfer, which causes the electron cloud to overlap.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"13 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142189332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}