M. Molanouri Shamsi, S. Soudi, M.T. Afshounpour, A. Jamali, H. Soleimanjahi, L.S. Quinn, R. Gharakhanlou, H. Karimi
{"title":"Effects of single and multiple bouts of eccentric exercise on immune responses to HSV-gD1 DNA vaccination in mice","authors":"M. Molanouri Shamsi, S. Soudi, M.T. Afshounpour, A. Jamali, H. Soleimanjahi, L.S. Quinn, R. Gharakhanlou, H. Karimi","doi":"10.1163/17552559-20220060","DOIUrl":"https://doi.org/10.1163/17552559-20220060","url":null,"abstract":"DNA vaccines comprise one modality of novel vaccination techniques. DNA vaccines employ intramuscular injection of DNA that codes for specific antigens from a pathogen. Prolonged eccentric endurance exercise can stimulate immune responses, possibly by increasing inflammatory mediators. This study aimed to determine the effects of one or three sessions of downhill running on the effectiveness of Herpes simplex virus type I glycoprotein D (HSV-gD1) DNA vaccination. Mice without or with one and three sessions of downhill running were vaccinated intramuscularly with the DNA vaccine or the empty vector. Cellular immune responses were assessed using an in vitro assay of lymphocyte proliferation in response to recall antigens. Also, a virus neutralisation test was used to determine the levels of neutralising antibodies in serum samples. In addition, Th1 and Th2 immune responses, and the ratio of the Th1/Th2 is considered an indicator of immune responses to infection in the current study. Multiple bouts of downhill running increased humoral immune responses after immunisation, also although Th2 immune responses decreased, lymphocyte proliferation and Th1/Th2 ratio immune responses increased following the viral challenge compared to the other groups. In addition, the timing of the 3-session exercise regimen plus DNA vaccination immediately following the last exercise session, as opposed to DNA vaccination 48 h before a single session of exercise, may account for the greater efficacy of DNA vaccination in the multiple bouts of the intensive exercise group. These findings indicate that short-term repeated bouts of eccentric endurance exercise have increased immune responses to intramuscular DNA-based vaccines.","PeriodicalId":10709,"journal":{"name":"Comparative Exercise Physiology","volume":"49 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139323494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Relationship between body condition and vertical movement symmetry in 109 riding school horses","authors":"E. Persson-Sjödin, E. Wärnsberg, S. Ringmark","doi":"10.1163/17552559-20220068","DOIUrl":"https://doi.org/10.1163/17552559-20220068","url":null,"abstract":"For riding schools, the health and function of their horses is paramount. Unfortunately, lameness and overweight are common problems in this population, and a causal relationship whereby increased body weight causes lameness is also possible. This observational field study investigated the relationship between body condition score (BCS) and motion movement asymmetries in riding school horses and also explored the relationships between BCS, movement asymmetry and subjectively scored willingness to work. Eight riding schools within 2.5 h by car from Uppsala, Sweden, were convenience-sampled and 3-24 horses from each were included in the study. Body condition was scored (scale 1-9) and vertical movement asymmetry data were collected in straight-line trot, using an inertial measurement unit system. Asymmetry parameters were calculated as the mean difference between local vertical displacement minima/maxima for the head/pelvis (HD/PD) between the two halves of the stride. A questionnaire was used to assess perceived willingness to work and energy level of the horses. The data were analysed with linear mixed models, t-tests and Mann-Whitney U-tests. A correlation was found between BCS and PDmin (), an asymmetry parameter related to hindlimb weight-bearing lameness, where PDmin increased by 0.96 mm for each unit increase in BCS. Horses with vertical movement classified as symmetric had lower BCS than horses with movement classified as asymmetric (5.4 ± 0.4 vs 5.7 ± 0.6, ). Horses classed as overweight (BCS ≥ 6) scored lower on willingness to work () and energy level (). These results indicate that the body condition of riding school horses may influence their function and the health of their locomotor apparatus. Further studies are required to identify the underlying mechanisms.","PeriodicalId":10709,"journal":{"name":"Comparative Exercise Physiology","volume":"50 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139334100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Legg, D.J. Cochrane, E. Gee, M. Breheny, J. Richards, Y. Chin, C. Rogers
{"title":"Identification of the challenges to and attitudes of apprentice jockeys towards implementation of specialised exercise training in New Zealand","authors":"K. Legg, D.J. Cochrane, E. Gee, M. Breheny, J. Richards, Y. Chin, C. Rogers","doi":"10.1163/17552559-20230012","DOIUrl":"https://doi.org/10.1163/17552559-20230012","url":null,"abstract":"Jockeys exercise at maximal physiological capacity during a race, multiple times each race day, whilst maintaining a low body weight. Therefore, understanding the physical demands of jockeys during training and competition, along with development of specific exercise programmes is important in preparing and supporting the performance, physical well-being and career progression of these athletes. This study aimed to develop and implement a targeted, jockey specific training (JET – Jockey Exercise Training) programme to assess the engagement and attitudes of a cohort of 50 apprentice jockeys to an off-horse training programme. The JET programme consisted of 8 weeks of tri-weekly training exercises, delivered via video recording through a social media platform (Facebook) and fortnightly in-person sessions. Uptake of JET was poor, with only 10% of jockeys completing over half of the recommended number of JET trainings. The reasons for this were explored via brief structured interviews using cue cards, with 16 apprentice jockeys. Most of the apprentices valued the importance of the increased physical fitness provided by JET. However, they lacked self-motivation and believed they already did enough physical fitness during their workday commitments. The introduction of group sessions, providing both social accountability and support increased JET programme uptake. Industry modification to promote the social context of valuing the physical well-being of jockeys as elite athletes in all industry participants, in combination with early introduction of jockey specific training in a structured framework, may be a key component to the successful introduction of future jockey exercise programmes.","PeriodicalId":10709,"journal":{"name":"Comparative Exercise Physiology","volume":"14 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139334353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Performance analysis of show jumping rounds at a national pony competition","authors":"C. Gluck, J. Williams, S. Pratt-Phillips","doi":"10.1163/17552559-20220064","DOIUrl":"https://doi.org/10.1163/17552559-20220064","url":null,"abstract":"Performance analysis is utilised by coaches and athletes to identify areas to work on in training and to identify strengths in athlete performance in various sports. However, performance analysis is not commonly used within equestrian sports. The purpose of this study was to evaluate minors and their ponies competing in show jumping at a national pony competition to see if course variables affected performance. All jumping rounds were watched online. Type of faults (e.g. rails, refusals, time faults, fall of horse and or rider), type of fence (e.g. vertical, oxer), approach angle, section of the course where fault(s) occurred and round time were recorded. Spearman’s Correlation assessed if round time was correlated to total faults and a series of Kruskal-Wallis analyses determined if significant differences in faults occurred between sections of the course, where these existed, post hoc tests established where differences occurred between rounds. There was no significant difference in total faults across the 4 rounds of competition and no meaningful correlation between round time and total faults (r = 0.34; ). There were no differences between fence type and faults although more faults occurred at verticals (51.7%, n = 46 faults at verticals versus 48.3%, n = 43 at oxers; ). Faults were more likely to occur during the final quarter of the course (32.6%, n = 29) when compared to the first quarter (23.6%, n = 21; ). These results showed that faults were more likely to occur in the final quarter of a round. The information gained from this performance analysis could be beneficial to inform training or riding strategies, especially when preparing for a competition.","PeriodicalId":10709,"journal":{"name":"Comparative Exercise Physiology","volume":"26 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139334693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of upper extremity kinesio tape application on hand function, wrist joint position and grip strength in children with hemiparetic cerebral palsy","authors":"D. Yılmaz, M. Yıldız, M.S. Yıldırım, B. Karaoğlu","doi":"10.1163/17552559-20230011","DOIUrl":"https://doi.org/10.1163/17552559-20230011","url":null,"abstract":"Although kinesio taping (KT) is frequently used in musculoskeletal disorders, there is not enough data on the effectiveness of holistic KT application on the upper extremity in spastic hemiparetic children. Our study aims to evaluate the effect of KT application on spasticity, range of motion, dexterity and hand grip strength in these patients. Twenty patients diagnosed with spastic hemiparetic cerebral palsy, aged 5-17 years, who were trained and treated in a private educational institution, were included in the study. Between November 2021 and January 2022, in addition to neurodevelopmental therapy for 12 weeks, KT was applied from the lateral epicondyle region to the end of the metacarpals, with 15-25% tension, to the dorsal face of the hand, with the taping direction facilitation technique (starting direction is the origin of the muscle). Spasticity, range of motion, dexterity and hand grip strength were evaluated as pre-test and post-test. It was determined that the mean score difference between the pre-test and post-test range of motion (ROM) shoulder abduction, ROM supination, ROM wrist flexion, ROM wrist extension and hand grip strength of the individuals was statistically significant (). It was determined that the individuals’ pre-test-post-test Jebsen hand function test (JHFT) Writing, JHFT card flipping, JHFT small objects, JHFT nutrition simulation, JHFT backgammon checkers stamps, JHFT large light objects, JHFT total score average difference was statistically significant (). These results indicate that KT application positively affects upper extremity functions in the rehabilitation of hemiparetic cerebral palsy. Longitudinal studies are recommended in patients with hemiparetic cerebral palsy.","PeriodicalId":10709,"journal":{"name":"Comparative Exercise Physiology","volume":"8 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. W. Wigati, M.P. Bintari, P. S. Rejeki, C.D.K. Wungu, A. Pranoto, R. N. Ramadhan, H. K. Setiawan, M. Munir, S. Halim
{"title":"The effect of 4 week-long swimming exercise intervention on increased serotonin levels in male mice (Mus musculus)","authors":"K. W. Wigati, M.P. Bintari, P. S. Rejeki, C.D.K. Wungu, A. Pranoto, R. N. Ramadhan, H. K. Setiawan, M. Munir, S. Halim","doi":"10.1163/17552559-20230005","DOIUrl":"https://doi.org/10.1163/17552559-20230005","url":null,"abstract":"Being overweight can be caused by an imbalance between calorie intake and energy expenditure over a long period, and the brain plays an important role in controlling overeating responses. Serotonin is a neurotransmitter involved in regulating food intake and weight. Being overweight is associated with decreased serotonin levels. Decreased serotonin levels have an impact on increased health disorders and depression. The study aimed to analyse the effect of 4 week-long swimming exercise intervention on increased serotonin levels in male mice (Mus musculus). A total of 18 male mice (20-40 g), aged eight weeks with (Lee index (LI) ≥0.3) randomly assigned to three groups: COG (n = 6; control group without intervention), MEG (n = 6; moderate-intensity morning exercise group), and AEG (n = 6; moderate-intensity afternoon exercise group). Morning and afternoon exercise was carried out with a load of 5% of the total body weight of mice. Four weeks of exercise with a frequency of three times a week and were performed for 70% of the maximum duration per session. Blood collection was done 2 × 24 h after the last intervention. Measurement of serum serotonin levels using ELISA. Data analysis techniques using the one-way ANOVA test with SPSS version 17 software. The average levels of serum serotonin of COG were (56.29 ± 3.59 ng/ml), MEG (86.21 ± 18.44 ng/ml), AEG (82.73 ± 25.15 ng/ml), and (). The results of this study show that moderate-intensity exercises in the morning and afternoon increase serum serotonin levels in male mice. Therefore, moderate-intensity morning and afternoon swimming exercises can be used in controlling and inhibiting the over-eating response mediated by the serotonin.","PeriodicalId":10709,"journal":{"name":"Comparative Exercise Physiology","volume":"2019 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139342993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Yuliastrid, N. Kusnanik, B. Purwanto, A. Noordia, S.P. Purwoto, A. Pranoto
{"title":"Single bout of a long-duration running treadmill increases myoglobin but not haemoglobin and interleukin 6 levels in mice (Mus musculus)","authors":"D. Yuliastrid, N. Kusnanik, B. Purwanto, A. Noordia, S.P. Purwoto, A. Pranoto","doi":"10.1163/17552559-20220075","DOIUrl":"https://doi.org/10.1163/17552559-20220075","url":null,"abstract":"Endurance was essential to perform an activity for as long as possible. Previously, haemoglobin, myoglobin, lactate and interleukin (IL)-6 level played a key role in determining endurance. This study aims to analyse the differences in the duration of running a treadmill on the levels of haemoglobin, myoglobin, lactate, and IL-6 in mice (Mus musculus). The mice were forced to run on the treadmill for as long as possible. They were divided into 3 groups, namely Short (n = 12; ≤12.5 min treadmill running), Moderate (n = 12; 12.5-40 min treadmill running), and Long (n = 12; ≥40 min treadmill running). Haemoglobin and lactate levels were evaluated, respectively, using the Quick-check Plus Hb Haemoglobin Testing System and Accutrend® Plus system. Analysis of myoglobin and IL-6 levels used an enzyme-linked immunosorbent assay (ELISA) kit. Statistical analysis used the one-way ANOVA, and LSD post-hoc tests with a significance level of 5%. The results showed that only the myoglobin level characterised endurance. Higher myoglobin levels were found at longer duration of running. Haemoglobin, lactate and IL-6 levels did not differ between groups. Based on the results of the study it was concluded that a longer duration of running was found a higher level of myoglobin. Myoglobin but not haemoglobin represented as an essential characteristic for endurance among runners in mice. High-affinity binding between myoglobin and oxygen was essential for oxygen storage in muscle.","PeriodicalId":10709,"journal":{"name":"Comparative Exercise Physiology","volume":"293 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139345082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Frippiat, C. van Beckhoven, V.J. van Gasselt, A. Dugdale, J.M. Vandeweerd
{"title":"Effect of gait on, and repeatability of heart rate and heart rate variability measurements in exercising Warmblood dressage horses","authors":"T. Frippiat, C. van Beckhoven, V.J. van Gasselt, A. Dugdale, J.M. Vandeweerd","doi":"10.1163/17552559-20220044","DOIUrl":"https://doi.org/10.1163/17552559-20220044","url":null,"abstract":"Heart rate variability (HRV) is a non-invasive measurement of the balance between the sympathetic and parasympathetic innervation to the myocardium. Used as an indicator of health, welfare and fitness level, its measurement during exercise has been suggested to be helpful in sport horses. The aims of this study were to assess the within- and between-day repeatability of time-domain HRV-measurements during exercise, and to evaluate the effect of gait (as a factor of increasing exercise intensity) on HRV. Heart rate (HR), root mean square of successive differences (RMSSD) between normal heartbeats and the standard deviation of normal (R-R) intervals (SDNN) were assessed during a standardised exercise test (SET) on five consecutive days in a sample of 21 healthy Warmblood dressage horses. All variables showed good between-day repeatability (Spearman’s rank correlation coefficient), while only RMSSD showed reliable within-day repeatability (Wilcoxon signed-rank test). Increase of exercise intensity was significantly associated with increased HR and decreased RMSSD and SDNN, with a negative correlation between HR and the two HRV-measurements. HR and HRV values varied significantly with intensity of exercise (rest, walk, trot, canter and whole SET), though HRV at rest was not different to that at walk, and HRV at trot was not different to that at canter. In conclusion, the results of this study support the use and show the repeatability of time-domain HRV-measurements during exercise in Warmblood dressage horses. Further investigation for use of HRV in other equestrian disciplines, levels and health state is warranted.","PeriodicalId":10709,"journal":{"name":"Comparative Exercise Physiology","volume":"27 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139348466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The 9th International Conference on Canine and Equine Locomotion, Utrecht, The Netherlands 2023","authors":"","doi":"10.3920/cep2023.s1","DOIUrl":"https://doi.org/10.3920/cep2023.s1","url":null,"abstract":"Abstracts of the The 9th International Conference on Canine and Equine Locomotion, Utrecht, The Netherlands 2023.","PeriodicalId":10709,"journal":{"name":"Comparative Exercise Physiology","volume":"116 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83944757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}