William Davison , Michael Axelsson , Stefan Nilsson , Malcolm E. Forster
{"title":"Cardiovascular control in Antarctic notothenioid fishes","authors":"William Davison , Michael Axelsson , Stefan Nilsson , Malcolm E. Forster","doi":"10.1016/S0300-9629(97)86789-8","DOIUrl":"https://doi.org/10.1016/S0300-9629(97)86789-8","url":null,"abstract":"<div><p>The temperatures in the polar oceans are not only low, but also relatively stable. Blood becomes more viscous at cold temperatures and it is assumed that this increase of viscosity is responsible for a number of adaptations of the cardiovascular system. The Antarctic Nototheniids show large changes in haematocrit compared to other fishes, and this phenomenon may be related to the high viscosity of their blood at low temperatures. Reduction of the haematocrit will reduce the viscosity, and thus diminish cardiac work. Indeed, one group (the “icefish,” Channichthydae) has disposed of erythrocytes altogether.</p><p>The cholinergic tonus on the heart is remarkably high under “resting” conditions—up to 80% in the bottom-dwelling <em>Trematomus bemacchii</em>—and changes in cardiac performance appear to depend chiefly on modulation of this tonus, rather than activity in excitatory (adrenergic) fibres. Sequestering of erythrocytes by the spleen is a major factor in the reduction of haematocrit, and cholinergic autonomic nerves control release of these cells to increase haematocrit during periods of demand. Thus, the studies of the autonomic control of the heart and spleen of the Antarctic fish show that these are unusual among fishes in that both organs appear more or less solely cholinergically controlled.</p></div>","PeriodicalId":10612,"journal":{"name":"Comparative Biochemistry and Physiology Part A: Physiology","volume":"118 4","pages":"Pages 1001-1008"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-9629(97)86789-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72261201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Pisano , C. Ozouf-Costaz , C. Bonillo , A. Caimo , S. Rossetti , R. Williams
{"title":"Cytogenetics of the Antarctic icefish Champsocephalus gunnari Lönnberg, 1905 (Channichthyidae, Notothenioidei)","authors":"E. Pisano , C. Ozouf-Costaz , C. Bonillo , A. Caimo , S. Rossetti , R. Williams","doi":"10.1016/S0300-9629(97)86797-7","DOIUrl":"https://doi.org/10.1016/S0300-9629(97)86797-7","url":null,"abstract":"<div><p>Traditional karyotyping and molecular cytogenetic techniques were used to study the chromosomes of <em>Champsocephalus gunnari</em> from the Indian Sector of the Antarctic Ocean. <em>C. gunnari</em> has 24 pairs of chromosomes, giving a diploid number of 48. Most are acrocentric, six are metacentric and submetacentric and the level of divergence from the supposed ancestral notothenioid karyotype remains low. The chromosomal features of this icefish do not differ very much from those of the other channichthyids, the karyotypic macrostructure being kept stable in these fishes. Although providing relevant basic biological information, traditional cytogenetics do not allow a significant comparative analysis between species in this notothenioid family. Molecular cytogenetic techniques such as <em>in situ</em> hybridization have been successfully used on <em>C. gunnari</em>, opening new opportunities for a deeper characterization of Antarctic fish chromosomes. The <em>in situ</em> location of specific DNA sequences could provide valuable new information for phylogenetic reconstruction, and shed light on the relationship between chromosome change and the evolutionary process.</p></div>","PeriodicalId":10612,"journal":{"name":"Comparative Biochemistry and Physiology Part A: Physiology","volume":"118 4","pages":"Pages 1087-1094"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-9629(97)86797-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72261203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Volume contents, subject, and author index for vol. 118A","authors":"","doi":"10.1016/S0300-9629(97)86808-9","DOIUrl":"https://doi.org/10.1016/S0300-9629(97)86808-9","url":null,"abstract":"","PeriodicalId":10612,"journal":{"name":"Comparative Biochemistry and Physiology Part A: Physiology","volume":"118 4","pages":"Pages III-X"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-9629(97)86808-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72261241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of diet on the biochemical composition of Paracentrotus lividus (Echinodermata: Echinoidea) under natural and rearing conditions (effect of diet on biochemical composition of urchins)","authors":"C. Fernandez","doi":"10.1016/S0300-9629(97)00221-1","DOIUrl":"https://doi.org/10.1016/S0300-9629(97)00221-1","url":null,"abstract":"","PeriodicalId":10612,"journal":{"name":"Comparative Biochemistry and Physiology Part A: Physiology","volume":"45 1","pages":"1377-1384"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73382903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Scott L. Shofer , James A. Willis , Ronald S. Tjeerdema
{"title":"Effects of hypoxia and toxicant exposure on phosphoarginine, intracellular pH, and free Mg2+ in abalone as measured by 31P-NMR","authors":"Scott L. Shofer , James A. Willis , Ronald S. Tjeerdema","doi":"10.1016/S0300-9629(97)00061-3","DOIUrl":"10.1016/S0300-9629(97)00061-3","url":null,"abstract":"<div><p>The effects of hypoxia, sodium azide and pentachlorophenol (PCP) exposure on high-energy phosphorylated compounds, intracellular pH (pH<sub>i</sub>) and intracellular free Mg<sup>2+</sup> (Mg<sub>f</sub>) in intact red abalone (<em>Haliotis rufescens</em>) were determined using <sup>31</sup>P-NMR. Abalone made hypoxic by bubbling sea water with N<sub>2</sub> showed modest changes in phosphoarginine (PA) and inorganic phosphate (P<sub>i</sub>) concentrations, no significant changes in pH<sub>i</sub> and a moderate decrease in Mg<sub>f</sub> that was not statistically significant. Azide (50 mg/l) exposed animals displayed severe declines in PA dropping to 0.53 of reference values, coupled with large increases in P<sub>i</sub> to 10.66 times resting concentrations that occurred just after the 2-hr exposure period. pH<sub>i</sub> also showed significant declines from a resting value of 7.17-7.06 (<em>P</em> < 0.05) but fully recovered by the end of the 6-hr clean seawater recovery period, whereas Mg<sub>f</sub> concentrations declined slightly during the exposure period but increased by 18% at the end of the recovery period relative to reference Mg<sub>f</sub>. PCP (1.2 mg/l) exposed animals displayed similar increases and declines in P<sub>i</sub> and PA, respectively, as did azide-exposed animals by the end of the exposure period, but recovery was much slower and occurred in a bimodal fashion with some animals completely recovering at the end of 6 hr and others essentially stabilized at the end of the exposure period and did not show any significant changes during the recovery period.</p></div>","PeriodicalId":10612,"journal":{"name":"Comparative Biochemistry and Physiology Part A: Physiology","volume":"118 4","pages":"Pages 1183-1191"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-9629(97)00061-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79517700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isis Fernández, M. Oliva, O. Carrillo, A. Wormhoudt
{"title":"Digestive enzyme activities of Penaeus notialis during reproduction and moulting cycle","authors":"Isis Fernández, M. Oliva, O. Carrillo, A. Wormhoudt","doi":"10.1016/S0300-9629(97)86802-8","DOIUrl":"https://doi.org/10.1016/S0300-9629(97)86802-8","url":null,"abstract":"","PeriodicalId":10612,"journal":{"name":"Comparative Biochemistry and Physiology Part A: Physiology","volume":"10 1","pages":"1267-1271"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78786301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of temperature on the activities of glucose-6-phosphate dehydrogenase and hexokinase in entomopathogenic nematodes (Nematoda: Steinernematidae)","authors":"Ganpat B. Jagdale , Roger Gordon","doi":"10.1016/S0300-9629(97)00034-0","DOIUrl":"10.1016/S0300-9629(97)00034-0","url":null,"abstract":"<div><p>The kinetic properties of two metabolic enzymes, glucose-6-phosphate dehydrogenase and hexokinase, were studied in four strains of entomopathogenic nematodes that had been recycled for two years at various temperatures: <em>Steinernema feltiae</em> NF strain, <em>Steinernema feltiae</em> Umeå strain, <em>Steinernema carpocapsae</em> All strain, <em>Steinernema riobravis</em> TX strain. The recycling temperatures influenced the activities of glucose-6-phosphate dehydrogenase and hexokinase in an adaptive fashion in all the strains. At each assay temperature (5–35°C), the maximum specific activity of both the enzymes was greater in the nematodes that had been recycled at lower temperatures than in those reared at higher temperatures. In three enzyme-nematode strain combinations, the lowest K<sub>m</sub> values measured at each assay temperature occurred in nematodes that had been recycled at the lower temperatures. However, the assay temperatures at which the minimum K<sub>m</sub> values occurred were ≥15°C. The capacities of these nematodes to adjust to different recycling temperatures is discussed in relation to the physiological mechanisms involved.</p></div>","PeriodicalId":10612,"journal":{"name":"Comparative Biochemistry and Physiology Part A: Physiology","volume":"118 4","pages":"Pages 1151-1156"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-9629(97)00034-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20428872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Regulation of body water balance in reedfrogs (superspecies Hyperolius viridiflavus and Hyperolius marmoratus: Amphibia, anura, hyperoliidae) living in unpredictably varying savannah environments","authors":"R. Schmuck, K.E. Linsenmair","doi":"10.1016/S0300-9629(97)86804-1","DOIUrl":"10.1016/S0300-9629(97)86804-1","url":null,"abstract":"<div><p>The regulation of body water balance was examined in the reedfrogs <em>Hyperolius marmoratus taeniatus</em> and <em>Hyperolius viridiflavus ommatostictus</em>. Temperature and stage of post-metamorphic development significantly affected the rate of water uptake. Hydrated reedfrogs prevented hyperhydration by voiding diluted urine when obtaining water. Within 48 hr after rehydration, body fluid osmolality remained at low levels, which may be supportive to counter excessive cutaneous water influx in hydrated frogs. Once evaporative water loss exceeded 10–12% total body mass, reedfrogs became anuric. The rate of water uptake strongly increased with increasing body water deficit. Both the anuric response and the increased rate of water uptake are assumed to strongly enhance the efficacy of using very briefly available water sources during dry-period conditions. Dry-adapted and estivating reedfrogs survived evaporative water losses between 40 and 55% total body mass. Bladder fluid stores contributed substantially to this desiccation tolerance. During a 16-day period of desiccation, <em>H. v. ommatostictus</em> could replace approximately 25% of evaporative water loss from the bladder fluid store. During desiccation, the level of free amino acids selectively increased in the gastrocnemius muscle tissue, which may support cell volume regulation and/or protect cellular structures from osmotic stresses. Even strongly dehydrated reedfrogs rehydrated quickly with no obvious osmoregulatory problem. Rehydration was associated with a higher than expected decrease of free amino acids in the gastrocnemius muscle tissue, a response that may help to protect cells from bursting during fast rehydration.</p></div>","PeriodicalId":10612,"journal":{"name":"Comparative Biochemistry and Physiology Part A: Physiology","volume":"118 4","pages":"Pages 1335-1352"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-9629(97)86804-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20429494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative Physiology of the l-Arginine-Nitric Oxide Pathway: An Overview of a Symposium at the 4th International Congress of Comparative Physiology and Biochemistry, 1995","authors":"Maurice R. Elphick , René Schleiffer","doi":"10.1016/S0300-9629(97)00021-2","DOIUrl":"10.1016/S0300-9629(97)00021-2","url":null,"abstract":"","PeriodicalId":10612,"journal":{"name":"Comparative Biochemistry and Physiology Part A: Physiology","volume":"118 4","pages":"Pages 921-923"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-9629(97)00021-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87808356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Different binding activities of A- and B-type natriuretic hormones in the heart of two Antarctic teleosts, the red-blooded Trematomus bernacchii and the hemoglobinless Chionodraco hamatus☆","authors":"M. Cerra, M. Canonaco, R. Acierno, B. Tota","doi":"10.1016/S0300-9629(97)86788-6","DOIUrl":"https://doi.org/10.1016/S0300-9629(97)86788-6","url":null,"abstract":"","PeriodicalId":10612,"journal":{"name":"Comparative Biochemistry and Physiology Part A: Physiology","volume":"29 1","pages":"993-999"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88180161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}