Clinical Diagnostic Laboratory Immunology最新文献_第2页

Use of Recombinant Envelope Proteins for Serological Diagnosis of Dengue Virus Infection in an Immunochromatographic Assay 利用重组包膜蛋白免疫层析检测登革病毒感染的血清学诊断

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-11-01 DOI: 10.1128/CDLI.8.6.1150-1155.2001

A. Cuzzubbo, T. Endy, A. Nisalak, S. Kalayanarooj, D. Vaughn, Steven A. Ogata, D. Clements, P. Devine

引用次数: 106

Chlamydia pneumoniae Exacerbates Aortic Inflammatory Foci Caused by Murine Cytomegalovirus Infection in Normocholesterolemic Mice 肺炎衣原体加重正常胆固醇血症小鼠巨细胞病毒感染引起的主动脉炎症灶

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-11-01 DOI: 10.1128/CDLI.8.6.1263-1266.2001

K. Burián, K. Berencsi, V. Endrész, Z. Gyulai, T. Valyi-Nagy, I. Vályi-Nagy, M. Bakay, Yuemei Geng, D. Virok, L. Kari, R. Hajnal-Papp, G. Trinchieri, E. Gonczol

引用次数: 26

Prevalence of Hepatitis E Virus Antibodies in Canadian Swine Herds and Identification of a Novel Variant of Swine Hepatitis E Virus 加拿大猪群戊型肝炎病毒抗体的流行及猪戊型肝炎病毒新变种的鉴定

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-11-01 DOI: 10.1128/CDLI.8.6.1213-1219.2001

D. Yoo, Philip Willson, Y. Pei, M. A. Hayes, A. Deckert, C. Dewey, R. Friendship, Y. Yoon, M. Gottschalk, C. Yason, A. Giulivi

{"title":"Prevalence of Hepatitis E Virus Antibodies in Canadian Swine Herds and Identification of a Novel Variant of Swine Hepatitis E Virus","authors":"D. Yoo, Philip Willson, Y. Pei, M. A. Hayes, A. Deckert, C. Dewey, R. Friendship, Y. Yoon, M. Gottschalk, C. Yason, A. Giulivi","doi":"10.1128/CDLI.8.6.1213-1219.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.6.1213-1219.2001","url":null,"abstract":"ABSTRACT Swine hepatitis E virus is a newly identified potentially zoonotic virus from pigs of particular concern for possible direct transmission to a human xenotransplant recipient by organ transplantation. In the present study, prevalence of serum antibodies to hepatitis E virus was examined in Canadian swine herds. A total of 998 serum samples collected from 6-month-old healthy slaughter hogs were examined by enzyme immunoassay and Western blot analysis for antibodies to the recombinant open reading frame 3 (ORF3) protein of hepatitis E virus expressed in Escherichia coli. These samples represented more than 80 different swine production units from five major swine-producing provinces across Canada. From this study, 594 samples (59.4%) were found to be positive for hepatitis E virus antibody. The seroprevalence was higher in Quebec (88.8%) and Ontario (80.1%) than in Alberta and Saskatchewan (38.3%). By PCR using a pair of oligonucleotide primers deduced from the ORF2 sequence of human hepatitis E virus, a specific hepatitis E virus sequence was recovered from feces of pigs. The nucleotide sequence identity between the U.S. swine hepatitis E virus and the Canadian isolate (SK3) was only 85.8%, suggesting that genotypic variations may exist in swine hepatitis E virus in North America. Among 165 serum samples collected from humans in Saskatchewan, 2.4% were found to be positive for antibodies to the hepatitis E virus ORF3 protein. Our data indicate that hepatitis E virus is highly prevalent in commercial swine populations in Canada and support the suggestion that the swine hepatitis E virus may be an important zoonotic agent for humans.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"44 1","pages":"1213 - 1219"},"PeriodicalIF":0.0,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86723882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 108

Expression of a Novel Protein by Regenerating Hepatocytes and Peripheral Blood Lymphocytes 一种新蛋白在再生肝细胞和外周血淋巴细胞中的表达

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-11-01 DOI: 10.1128/CDLI.8.6.1292-1294.2001

A. Chedid, C. Sung, M. R. Lepe, S. A. Ahmed, Syeda A. Iftikhar, A. Feller, K. Beaman

引用次数: 2

Determinants of Staphylococcusaureus Nasal Carriage 金黄色葡萄球菌鼻腔载体的决定因素

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-11-01 DOI: 10.1128/CDLI.8.6.1064-1069.2001

A. Cole, Samuel Tahk, A. Oren, D. Yoshioka, Yong-Hwan Kim, A. Park, T. Ganz

{"title":"Determinants of Staphylococcusaureus Nasal Carriage","authors":"A. Cole, Samuel Tahk, A. Oren, D. Yoshioka, Yong-Hwan Kim, A. Park, T. Ganz","doi":"10.1128/CDLI.8.6.1064-1069.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.6.1064-1069.2001","url":null,"abstract":"ABSTRACT Nasal carriage of Staphylococcusaureus has been identified as a risk factor for community-acquired and nosocomial infections. We screened 230 donors of diverse ethnic and socioeconomic backgrounds and identified 62 (27%) whose nasal secretions were colonized by S.aureus. In 18 donors in whom the various regions of the nasal luminal surface were separately sampled, the predominant region of S. aureus colonization was the moist squamous epithelium on the septum adjacent to the nasal ostium. Nasal fluid from carriers was defective in killing endogenousS. aureus and nasal carrier isolates ofS. aureus but not a laboratoryS. aureus strain. Transmission electron microscopy revealed that S.aureus isolates incubated in nasal fluid from carriers for 2 h at 37°C were less damaged than those incubated in noncarrier fluid and were coated with an electron-dense layer. Compared with that from healthy donors and patients with acute rhinitis, nasal fluid from carriers contained elevated concentrations of the neutrophil-derived defensins human neutrophil peptides 1 to 3 (47- and 4-fold increases, respectively), indicative of a neutrophil-mediated inflammatory host response to S.aureus colonization. The concentration of the inducible epithelial antimicrobial peptide human β-defensin 2 was also highly elevated compared to that in healthy donors, in whom the level was below the detection limit, or patients with acute rhinitis (sixfold increase). Thus, nasal carriage of S.aureus takes hold in nasal fluid that is permissive for colonization and induces a local inflammatory response that fails to clear the colonizing bacteria.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"19 1","pages":"1064 - 1069"},"PeriodicalIF":0.0,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89487954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 163

Antibody Responses to Acinetobacterspp. and Pseudomonas aeruginosa in Multiple Sclerosis: Prospects for Diagnosis Using the Myelin-Acinetobacter-Neurofilament Antibody Index 抗体对不动杆菌的反应。多发性硬化和铜绿假单胞菌:髓磷脂-不动杆菌-神经丝抗体指数诊断的前景

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-11-01 DOI: 10.1128/CDLI.8.6.1181-1188.2001

L. Hughes, S. Bonell, R. Natt, C. Wilson, H. Tiwana, A. Ebringer, P. Cunningham, V. Chamoun, E. Thompson, J. Croker, J. Vowles

引用次数: 39

Inhibition of Ganciclovir-Susceptible and -Resistant Human Cytomegalovirus Clinical Isolates by the Benzimidazole l-Riboside 1263W94 苯并咪唑l-核苷1263W94对更昔洛韦敏感和耐药人巨细胞病毒临床分离株的抑制作用

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-11-01 DOI: 10.1128/CDLI.8.6.1279-1281.2001

J. Mcsharry, Ann McDonough, B. Olson, C. Talarico, M. Davis, K. Biron

引用次数: 47

Modulation of Mycobacterium bovis-Specific Responses of Bovine Peripheral Blood Mononuclear Cells by 1,25-Dihydroxyvitamin D3 1,25-二羟基维生素D3对牛分枝杆菌对牛外周血单个核细胞特异性反应的调节

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-11-01 DOI: 10.1128/CDLI.8.6.1204-1212.2001

W. Waters, B. Nonnecke, T. Rahner, M. Palmer, D. Whipple, R. Horst

{"title":"Modulation of Mycobacterium bovis-Specific Responses of Bovine Peripheral Blood Mononuclear Cells by 1,25-Dihydroxyvitamin D3","authors":"W. Waters, B. Nonnecke, T. Rahner, M. Palmer, D. Whipple, R. Horst","doi":"10.1128/CDLI.8.6.1204-1212.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.6.1204-1212.2001","url":null,"abstract":"ABSTRACT Historically, administration of vitamin D has been considered beneficial in the treatment of tuberculosis. The interaction of this vitamin {i.e., 1,25-dihdroxyvitamin D3[1,25(OH)2D3]} with the antitubercular immune response, however, is not clear. In the present study, in vitro recall responses of peripheral blood mononuclear cells (PBMC) from cattle infected with Mycobacterium bovis were used to study the immune-modulatory effects of 1,25(OH)2D3 on M. bovis-specific responses in vitro. Addition of 1 or 10 nM 1,25(OH)2D3 inhibited M. bovis-specific proliferative responses of PBMC from M. bovis-infected cattle, affecting predominately the CD4+ cell subset. In addition, 1,25(OH)2D3 inhibited M. bovis-specific gamma interferon (IFN-γ) production yet enhanced M. bovis-specific nitric oxide (NO) production. Lymphocyte apoptosis, measured by flow cytometry using annexin-V staining, was diminished by addition of 1,25(OH)2D3 to PBMC cultures. These findings support the current hypothesis that 1,25(OH)2D3enhances mycobacterial killing by increasing NO production, a potent antimicrobial mechanism of activated macrophages, and suggest that 1,25(OH)2D3 limits host damage by decreasingM. bovis-induced IFN-γ production.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"13 1","pages":"1204 - 1212"},"PeriodicalIF":0.0,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84094573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

Evaluation of Chlamydia pneumoniae 43- and 53-Kilodalton Recombinant Proteins for Serodiagnosis by Western Blot 肺炎衣原体43和53千道尔顿重组蛋白血清免疫印迹检测的评价

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-11-01 DOI: 10.1128/CDLI.8.6.1231-1233.2001

L. Campbell, S. Roberts, Shiuichiro Inoue, L. Kong, C. Kuo

{"title":"Evaluation of Chlamydia pneumoniae 43- and 53-Kilodalton Recombinant Proteins for Serodiagnosis by Western Blot","authors":"L. Campbell, S. Roberts, Shiuichiro Inoue, L. Kong, C. Kuo","doi":"10.1128/CDLI.8.6.1231-1233.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.6.1231-1233.2001","url":null,"abstract":"ABSTRACT Chlamydia pneumoniae is a common cause of respiratory infection. It has also been shown to be associated with coronary heart disease. Two proteins that have been reported to be recognized frequently during human infection are proteins having molecular masses of 43 and 53 kDa. In order to develop a useful alternative serological test to the microimmunofluorescence (micro-IF) assay, recombinant 43-kDa and 53-kDa chlamydia-specific proteins were evaluated in dot blot and/or for comparison to the standard micro-IF test. Primers for amplification were derived from genome sequence information for two C. pneumoniae genes (CPn0809 and CPn0980) encoding 53-kDa proteins and four C. pneumoniae genes (CPn0562, CPn0927, CPn0928, and Cpn0929) encoding 43-kDa proteins of unknown function, which wereChlamydia specific and not found in Chlamydia trachomatis. The 53-kDa protein product of CPn0809 or the N-terminal 18-kDa portion had better specificity than any of the 43-kDa recombinants but was much less sensitive than micro-IF. In contrast, the 53-kDa protein encoded by CPn0980 was recognized by 11 of 12 (92%) acute-phase sera, 35 of 46 (76%) chronic sera, 0 of 12 micro-IF-negative sera (C. pneumoniae andC. trachomatis negative), and 1 of 12 (8%)C. pneumoniae negative, C. trachomatis positive sera. Thus, it appears that the 53-kDa protein encoded by CPn0980 has potential use for serodiagnosis ofC. pneumoniae infection.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"74 1","pages":"1231 - 1233"},"PeriodicalIF":0.0,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80001883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Mycobacterial Di-O-Acyl-Trehalose Inhibits Mitogen- and Antigen-Induced Proliferation of Murine T Cells In Vitro 分枝杆菌二氧酰基海藻糖体外抑制有丝分裂原和抗原诱导的小鼠T细胞增殖

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-11-01 DOI: 10.1128/CDLI.8.6.1-91-1088.2001

R. Saavedra, E. Segura, R. Leyva, Luis A. Esparza, L. López-Marín

{"title":"Mycobacterial Di-O-Acyl-Trehalose Inhibits Mitogen- and Antigen-Induced Proliferation of Murine T Cells In Vitro","authors":"R. Saavedra, E. Segura, R. Leyva, Luis A. Esparza, L. López-Marín","doi":"10.1128/CDLI.8.6.1-91-1088.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.6.1-91-1088.2001","url":null,"abstract":"ABSTRACT 2,3-Di-O-acyl-trehalose (DAT) is a glycolipid located on the outer layer of the Mycobacterium tuberculosis cell envelope. Due to its noncovalent linkage to the mycobacterial peptidoglycan, DAT could easily interact with host cells located in the focus of infection. The aim of the present work was to study the effects of DAT on the proliferation of murine spleen cells. DAT was purified from reference strains of M. tuberculosis,or M. fortuitum as a surrogate source of the compound, by various chromatography and solvent extraction procedures and then chemically identified. Incubation of mouse spleen cells with DAT inhibited in a dose-dependent manner concanavalin A-stimulated proliferation of the cells. Experiments, including the propidium iodide exclusion test, showed that these effects were not due to death of the cells. Tracking of cell division by labeling with 5,6-carboxyfluorescein diacetate succinimidyl ester revealed that DAT reduces the rounds of cell division. Immunofluorescence with an anti-CD3 monoclonal antibody indicated that T lymphocytes were the population affected in our model. Our experiments also suggest that the extent of the suppressive activity is strongly dependent on the structural composition of the acyl moieties in DATs. Finally, the inhibitory effect was also observed on antigen-induced proliferation of mouse spleen cells specific for Toxoplasma gondii. All of these data suggest that DAT could have a role in the T-cell hyporesponsiveness observed in chronic tuberculosis.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"19 1","pages":"1081 - 1088"},"PeriodicalIF":0.0,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88794068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42