Chronicles of Young Scientists最新文献_第5页

Simultaneous assessment and validation of reverse phase-high performance liquid chromatography method for quercetin, eugenol, myristicin, and safrole from nutmeg, fruit and mace 反相高效液相色谱法测定肉豆蔻、水果和豆蔻中槲皮素、丁香酚、肉豆蔻素和黄樟素的同时评价和验证

Chronicles of Young Scientists Pub Date : 2013-01-01 DOI: 10.4103/2229-5186.108797

D. Nagore, V. Kuber, P. Patil, T. Deshmukh

{"title":"Simultaneous assessment and validation of reverse phase-high performance liquid chromatography method for quercetin, eugenol, myristicin, and safrole from nutmeg, fruit and mace","authors":"D. Nagore, V. Kuber, P. Patil, T. Deshmukh","doi":"10.4103/2229-5186.108797","DOIUrl":"https://doi.org/10.4103/2229-5186.108797","url":null,"abstract":"Background: Nutmeg is the imperative spices having pharmacological importance. Objectives: The objective of this work was to standardize Nutmeg extract by RP-high performance liquid chromatography (HPLC) analysis. Settings and Design: An RP-HPLC method was developed for simultaneous quantification of quercetin (QUE), eugenol (EUG), myristicin (MYRS), and safrole (SAFR) from nutmeg fruit and mace extracts. Materials and Methods: RP-HPLC method was performed with Waters 2695 Alliance system using a 2996 photodiode array detector (PDA). QUE, EUG, MYRS, and SAFR were separated on a reverse-phase 250 × 4.6 mm, 5-m, Zorbax SB C18 column (Agilent). The mobile phase was prepared from 0.1% orthophosphoric acid in water of pH 2.5 (solvent-A) and acetonitrile (solvent-B). The gradient program was selected for separation. The PDA was set at 220 nm, which shows maximum response for all peaks. Statistical Analysis: Percent relative standard deviation (% RSD) and correlation coefficient (r 2 ) were calculated by standard formulas. Results: QUE, EUG, MYRS, and SAFR were satisfactorily resolved with retention time about 3, 7, 19 and 21 min. respectively. The method was validated and results obtained showed accepted values for correlation of coefficient and % RSD. Conclusions: The method was accurate and specific for analysis of nutmeg extract.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88983467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Comparison of Cronobacter sakazakii from Agra region grown in biofilms, agar surface associated and planktonic mode by proteomic analysis 阿格拉地区阪崎克罗诺杆菌在生物膜、琼脂表面相关和浮游模式下生长的蛋白质组学比较

Chronicles of Young Scientists Pub Date : 2013-01-01 DOI: 10.4103/2229-5186.108804

G. Sharma, A. Prakash

{"title":"Comparison of Cronobacter sakazakii from Agra region grown in biofilms, agar surface associated and planktonic mode by proteomic analysis","authors":"G. Sharma, A. Prakash","doi":"10.4103/2229-5186.108804","DOIUrl":"https://doi.org/10.4103/2229-5186.108804","url":null,"abstract":"Context: Cronobacter sakazakii is an emerging food borne pathogen that causes severe meningitis, meningoencephalitis, sepsis, and necrotizing enterocolitis in neonates and infants, with a high fatality rate. Aims: The present paper is for the rapid detection of C. sakazakii from milk and milk products of Agra region via PCR method and comparison of C. sakazakii in biofilm, on agar surface and planktonic cells by proteomic analysis. Materials and Methods: In the present study, 55 samples of milk and milk products of the Agra region were analyzed. 200 isolates were obtained of which 11 were biochemically detected as C. sakazakii . The PCR targeting the ompA gene was used to amplify a 496 bp DNA segment unique to C. sakazakii , in order to confirm C. sakazakii isolates. The proteome was investigated to study the differential protein pattern expressed by biofilm, agar surface-associated and planktonic bacteria employing SDS-PAGE. Statistical Analysis: UN-SCAN-6.1 gel analysis software. Results: The primer pair ESSF and ESSR was successfully used to amplify a 469 bp DNA unique to C. sakazakii . Whole cell protein profiles of planktonic, biofilm and agar surface associated were characteristic. Conclusion: The cultural procedure for detection of C. sakazakii is laborious, taking up to 7 days for completion, whereas PCR combined with enrichment culturing can detect C. sakazakii in about 12 hours and thus has the potential to be used as a rapid tool for detecting its presence. Differential protein pattern of C. sakazakii cultivated in biofilm versus agar-surface-associated and planktonic cells were observed. Further understanding the role of specific proteins during the biofilm development should permit a better understanding of the mechanisms sustaining the proliferation and the resistance of bacteria on biotic surfaces.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84291482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colesevelam: A novel drug for comorbid diabetes and dyslipidemia colesevilam:一种治疗合并糖尿病和血脂异常的新药

Chronicles of Young Scientists Pub Date : 2012-10-01 DOI: 10.4103/2229-5186.103102

A. Dixit, P. Pandey

引用次数: 0

Congenital maxillary double lip 先天性上颌双唇

Chronicles of Young Scientists Pub Date : 2012-10-01 DOI: 10.4103/2229-5186.103101

D. Chauhan, Y. Guruprasad

引用次数: 1

Sensitive spectroscopic method for content analysis of cefixime in solid dosage form using hydrotropy phenomenon 盐酸头孢克肟固体剂型中盐酸头孢克肟含量的敏感光谱分析方法

Chronicles of Young Scientists Pub Date : 2012-10-01 DOI: 10.4103/2229-5186.103099

Ethiraj Thiruvengadam, R. Ramadoss, M. Amudha

{"title":"Sensitive spectroscopic method for content analysis of cefixime in solid dosage form using hydrotropy phenomenon","authors":"Ethiraj Thiruvengadam, R. Ramadoss, M. Amudha","doi":"10.4103/2229-5186.103099","DOIUrl":"https://doi.org/10.4103/2229-5186.103099","url":null,"abstract":"Aim: The main aim of this present investigation is to apply the hydrotropic solubilization phenomenon for spectroscopic analysis of poorly water-soluble drugs to avoid the use organic solvents which may be costlier, toxic to environment, volatile, and pollutant. Materials and Methods: A simple ultra violet spectroscopic method was used for the content analysis by diluting the drug cefixime with various hydrotropic agents. In this study, 20% solutions of sodium salicylate (SS), sodium citrate (SC), sodium acetate (SA), and sodium benzoate (SB) were used as hydrotropes for the analysis of cefixime. Results and Discussion: The drug cefixime showed the linearity of 0.5-2 μg/mL in SS, 5-30 μg/mL in SC, 5-50 μg/mL in SA, and 0.05-0.30 μg/mL in SB solution. Then the proposed methods were validated with respect to accuracy and precision as per International Conference of Harmonization guidelines Q2 (R1), November 2005 (Validation of Analytical Procedures: Text and Methodology). The drug showed less limit of detection (LOD) and limit of quantification (LOQ) values (LOD = 0.0225471 μg/mL and LOQ 0.0683246 μg/mL) to SB solution and it obeyed the Beer's law at very low concentration range (0.05-0.30 μg/mL) which proved that the drug has high sensitivity with SB solution. Conclusions: Finally, it was concluded that the all proposed methods were simple, cost-effective, safe to environment, rapid, reproducible, and highly sensitive with SB solution.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78732747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Herpes virus: A key missing piece of the periodontopathogenic jigsaw puzzle 疱疹病毒:牙周病拼图中缺失的关键一环

Chronicles of Young Scientists Pub Date : 2012-10-01 DOI: 10.4103/2229-5186.103090

B. Pawar, Avneesh Tejnani, P. Marawar, A. Mani

引用次数: 4

RP-HPLC method for simultaneous estimation of propranolol hydrochloride and flunarizine dihydrochloride in their combined dosage formulation 盐酸心得洛尔与盐酸氟桂利嗪联合剂型中含量的反相高效液相色谱法测定

Chronicles of Young Scientists Pub Date : 2012-10-01 DOI: 10.4103/2229-5186.103094

B. Patel, A. K. Doshi, C. Patel

引用次数: 5

Nanomedicine to improve drug delivery outcomes [Retracted] 纳米医学改善给药效果[撤回]

Chronicles of Young Scientists Pub Date : 2012-10-01 DOI: 10.4103/2229-5186.103092

M. Joshi, G. Tiwari, R. Tiwari, B. Srivastava

{"title":"Nanomedicine to improve drug delivery outcomes [Retracted]","authors":"M. Joshi, G. Tiwari, R. Tiwari, B. Srivastava","doi":"10.4103/2229-5186.103092","DOIUrl":"https://doi.org/10.4103/2229-5186.103092","url":null,"abstract":"The early genesis of the concept of nanomedicine sprang from the visionary idea that tiny nanorobots and related machines could be designed, manufactured, and introduced into the human body to perform cellular repairs at the molecular level. Nanomedicine today has branched out in hundreds of different directions, each of them embodying the key insight that the ability to structure materials and devices at the molecular scale can bring enormous immediate benefits in the research and practice of medicine. The integration of nanotechnology with biology and medicine has given birth to a new field of science called Nanomedicine. Research into the rational delivery and targeting of pharmaceutical, therapeutic, and diagnostic agents is at the forefront of projects in nanomedicine. These involve the identification of precise targets (cells and receptors) related to specific clinical conditions and choice of the appropriate nanocarriers to achieve the required responses while minimizing the side effects. Mononuclear phagocytes, dendritic cells, endothelial cells, and cancers (tumor cells as well as tumor neovasculature) are key targets. The ultimate goal of nanomedicine is to develop well-engineered nanotools for the prevention, diagnosis, and treatment of many diseases. Nanomedicine today has branched out in hundreds of different directions, each of them embodying the key insight that the ability to structure materials and devices at the molecular scale can bring enormous immediate benefits in the research and practice of medicine.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81784513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Analysis of single-nucleotide polymorphisms of PEO1 gene in 55 ethnic groups of India 印度55个民族PEO1基因单核苷酸多态性分析

Chronicles of Young Scientists Pub Date : 2012-10-01 DOI: 10.4103/2229-5186.103100

Ashutosh Kumar Singh, A. Mitra, S. Rath

{"title":"Analysis of single-nucleotide polymorphisms of PEO1 gene in 55 ethnic groups of India","authors":"Ashutosh Kumar Singh, A. Mitra, S. Rath","doi":"10.4103/2229-5186.103100","DOIUrl":"https://doi.org/10.4103/2229-5186.103100","url":null,"abstract":"Background: Progressive External Opthalmoplegia (PEO1) or Chromosome 10 open reading frame 2 gene (OMIM ID 606075) encodes Twinkle protein, a phage T7 gene 4-like hexameric helicase, and is associated with mitochondrial DNA (mtDNA) deletions and neuromuscular disease called autosomal dominant PEO (adPEO). Twinkle has also been known to play an important role in the stability and maintenance of the mtDNA. Aims: In this study as an effort of Indian Genome Variation Consortium, we screened the SNPs of PEO1 gene such as rs7184, rs1535349, rs2863095, rs3740484, rs3740488, rs3740489, rs4919511, rs17113613, rs3824783, rs3740485, rs3740486, and rs3740487 in discovery panel (a population set of 40 DNA samples), and four synonymous SNPs, namely rs3824783 (ancestral allele=A), rs3740485 (ancestral allele=T), rs3740486 (ancestral allele=C), and rs3740487 (ancestral allele=A) in a large validation panel composed of 55 Indian subpopulations. Materials and Methods: In present study, a total of 55 Indian subpopulations were identified and collected for validation panel to check the frequencies of SNPs in PEO1 gene. Results and Conclusion: The allelic and genotype frequencies are found to be variable among different ethnic groups of India.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75735718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of stability indicating UPLC assay method for ziprasidone active pharma ingredient 齐拉西酮有效成分UPLC稳定性指示分析方法的建立与验证

Chronicles of Young Scientists Pub Date : 2012-10-01 DOI: 10.4103/2229-5186.103097

Sonam Mittal, Abhishek Gupta, B. Narasimhan, K. Srinivas, R. S. Gupta, V. P. Semwal

{"title":"Development and validation of stability indicating UPLC assay method for ziprasidone active pharma ingredient","authors":"Sonam Mittal, Abhishek Gupta, B. Narasimhan, K. Srinivas, R. S. Gupta, V. P. Semwal","doi":"10.4103/2229-5186.103097","DOIUrl":"https://doi.org/10.4103/2229-5186.103097","url":null,"abstract":"Background: Ziprasidone, a novel antipsychotic, exhibits a potent highly selective antagonistic activity on D2 and 5HT2A receptors. Literature survey for ziprasidone revealed several analytical methods based on different techniques but no UPLC method has been reported so far. Aim: Aim of this research paper is to present a simple and rapid stability indicating isocratic, ultra performance liquid chromatographic (UPLC) method which was developed and validated for the determination of ziprasidone active pharmaceutical ingredient. Forced degradation studies of ziprasidone were studied under acid, base, oxidative hydrolysis, thermal stress and photo stress conditions. Materials and Methods: The quantitative determination of ziprasidone drug was performed on a Supelco analytical column (100×2.1 mm i.d., 2.7 ΅m) with 10 mM ammonium acetate buffer (pH: 6.7) and acetonitrile (ACN) as mobile phase with the ratio (55:45-Buffer:ACN) at a flow rate of 0.35 ml/ min. For UPLC method, UV detection was made at 318 nm and the run time was 3 min. Developed UPLC method was validated as per ICH guidelines. Results and Conclusion: Mild degradation of the drug substance was observed during oxidative hydrolysis and considerable degradation observed during basic hydrolysis. During method validation, parameters such as precision, linearity, ruggedness, stability, robustness, and specificity were evaluated, which remained within acceptable limits. Developed UPLC method was successfully applied for evaluating assay of Ziprasidone active Pharma ingredient.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76187209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2