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cDNA Cloning and Sequence Analysis of Genome Segment S10 from Rice Black-Streaked Dwarf Virus 水稻黑条矮缩病毒基因组S10片段cDNA克隆及序列分析
Chinese Journal of Rice Science Pub Date : 2002-01-01 DOI: 10.1631/jzus.2002.0216
Zhang Heng-mu
{"title":"cDNA Cloning and Sequence Analysis of Genome Segment S10 from Rice Black-Streaked Dwarf Virus","authors":"Zhang Heng-mu","doi":"10.1631/jzus.2002.0216","DOIUrl":"https://doi.org/10.1631/jzus.2002.0216","url":null,"abstract":"Genome segment S10 of a Zhejiang isolate of rice black-streaked dwarf virus (RBSDV-Zj) was amplified by RT-PCR and sequenced. It consisted of 1801 nt (EMBL accession number is AJ297433) with only one open reading frame which encodes a polypeptide with molecular weight of 63.1 kD. Sequence comparison showed 93.8% and 96.2% homology at level of nucleotide and amino acid sequences between RBSDV-Zj and Japanese isolate (RBSDV-Jap), respectively;and shared 87.1% and 92.0% homology at level of nucleotide and acid sequences between RBSDV-Zj and Italian MRDV, respectively.","PeriodicalId":10160,"journal":{"name":"Chinese Journal of Rice Science","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81332271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Somaclonal Male-Sterile Mutants and Their Expressions in Indica Rice 体细胞无性系雄性不育突变体及其在籼稻中的表达
Chinese Journal of Rice Science Pub Date : 1990-01-01 DOI: 10.1142/9789812814272_0031
D. Ling, C. Liang, Z. Ma, M. F. Chen, B. He
{"title":"Somaclonal Male-Sterile Mutants and Their Expressions in Indica Rice","authors":"D. Ling, C. Liang, Z. Ma, M. F. Chen, B. He","doi":"10.1142/9789812814272_0031","DOIUrl":"https://doi.org/10.1142/9789812814272_0031","url":null,"abstract":"In past five years from 1984 to 1988, 48 cases of male sterile (ms) mutants in nine varieties were found, in which 30 cases belonged to R_1 generation and 28 to R_2 generation. The frequency of ms variation in R, generation was around 1% and 2% in R_2 generatiom. According to the characters of ms-mutants and their genetic expressions,pollen grain free(nuclear sterile) and abortive pollen (cytoplasmic genetic sterile) could be classified, In 1R54, two cases of pollen grain free ms-mutants were discovered similar in different generations (R_1 and R_2 generation) and different years (1985 and 1986) . Among ms-mutants of the abortive pollen type, one (54257) was found to be the cytoplasmic genetic male sterile mutant. Zheng-Shan 97, Er-Jiu-Ai,162-5 (a stable somaclonefrom IR52) and Gui-Chao 2 could maintain male sterility when crossed to the ms line (54257) . IR24, IR36, IR54,Shuang-Er-Zhan and others could restore its fertility. This result showed that \"54257\" was a cytoplasmic ms-mutant.This is the first sample that the cytoplasmic ms mutant could be induced by tissue culture in the world.","PeriodicalId":10160,"journal":{"name":"Chinese Journal of Rice Science","volume":"22 1","pages":"15-21"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88505487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
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