cDNA Cloning and Sequence Analysis of Genome Segment S10 from Rice Black-Streaked Dwarf Virus

Q3 Agricultural and Biological Sciences

Chinese Journal of Rice Science Pub Date : 2002-01-01 DOI:10.1631/jzus.2002.0216

Zhang Heng-mu

引用次数: 1

Abstract

Genome segment S10 of a Zhejiang isolate of rice black-streaked dwarf virus (RBSDV-Zj) was amplified by RT-PCR and sequenced. It consisted of 1801 nt (EMBL accession number is AJ297433) with only one open reading frame which encodes a polypeptide with molecular weight of 63.1 kD. Sequence comparison showed 93.8% and 96.2% homology at level of nucleotide and amino acid sequences between RBSDV-Zj and Japanese isolate (RBSDV-Jap), respectively;and shared 87.1% and 92.0% homology at level of nucleotide and acid sequences between RBSDV-Zj and Italian MRDV, respectively.

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水稻黑条矮缩病毒基因组S10片段cDNA克隆及序列分析

采用RT-PCR扩增水稻黑条矮缩病毒浙江分离株基因组S10片段，并对其序列进行了测定。它由1801个nt (EMBL登录号AJ297433)组成，只有一个开放阅读框，编码分子量为63.1 kD的多肽。序列比较显示，RBSDV-Zj与日本分离株(rbsdv - japan)在核苷酸和氨基酸水平上的同源性分别为93.8%和96.2%，与意大利MRDV在核苷酸和氨基酸水平上的同源性分别为87.1%和92.0%。

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来源期刊

Chinese Journal of Rice Science Agricultural and Biological Sciences-Plant Science

CiteScore

1.70

自引率

0.00%

发文量

2752

期刊介绍： Chinese Journal of Rice Science（CJRS）(ISSN 1001-7216), a bi-monthly journal published by China National Rice Research Institute, mainly publishes original research papers in fields of fundamental and applied fundamental scientific researches on rice, including genetics and breeding, germplasm resources, agronomy, bioengineering, plant protection, soil and plant nutrition, plant physiology, cereal chemistry and processing, agricultural economics and paddy field development, and agricultural engineering, etc. The journal also publishes reviews and experimental techniques. Articles are in Chinese with English abstract.