The Plant Pathology Journal最新文献_第5页

The Water-Soluble Chitosan Derivative, N-Methylene Phosphonic Chitosan, Is an Effective Fungicide against the Phytopathogen Fusarium eumartii 水溶性壳聚糖衍生物n -亚甲基膦壳聚糖是一种有效的抗植物致病菌镰刀菌剂

The Plant Pathology Journal Pub Date : 2021-06-16 DOI: 10.1101/2021.06.16.448680

F. A. Mesas, M. Terrile, M. Silveyra, A. Zuñiga, M. S. Rodríguez, C. Casalongué, J. Mendieta

{"title":"The Water-Soluble Chitosan Derivative, N-Methylene Phosphonic Chitosan, Is an Effective Fungicide against the Phytopathogen Fusarium eumartii","authors":"F. A. Mesas, M. Terrile, M. Silveyra, A. Zuñiga, M. S. Rodríguez, C. Casalongué, J. Mendieta","doi":"10.1101/2021.06.16.448680","DOIUrl":"https://doi.org/10.1101/2021.06.16.448680","url":null,"abstract":"Chitosan has been considered an environmental-friendly polymer. However, its use in agriculture has not been extended yet due to its relatively low solubility in water. In an attempt to improve such chemical characteristics, a chitosan-derivative prepared by adding a phosphonic group to chitosan N-methylene phosphonic chitosan, NMPC, was obtained from shrimp fishing industry waste from Argentinean Patagonia. This study showed that NMPC had a fungicidal effect on the phytopathogenic fungus Fusarium solani f. sp. eumartii (F. eumartii). NMPC inhibited F. eumartti mycelial growth and spore germination with low IC50 values. In vivo studies showed that NMPC affected fungal membrane permeability, ROS production, and cell death. NMPC also exerted antifungal effects against two other phytopathogens, Botrytis cinerea, and Phytophthora infestans. NMPC did not affect tomato cell viability at the same doses applied to these phytopathogens. Furthermore, the selective cytotoxicity of NMPC could give it added value in its application as an antimicrobial agent in agriculture.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128814409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II 气态二氧化氯对贮藏甘薯中尖孢镰刀菌体内外抑制作用的研究II

The Plant Pathology Journal Pub Date : 2019-10-01 DOI: 10.5423/PPJ.OA.04.2019.0078

Ye ji Lee, J. Jeong, Hyunjung Jin, Wook Kim, Y. Jeun, Gyeong-Dan Yu, Ki Deok Kim

{"title":"In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II","authors":"Ye ji Lee, J. Jeong, Hyunjung Jin, Wook Kim, Y. Jeun, Gyeong-Dan Yu, Ki Deok Kim","doi":"10.5423/PPJ.OA.04.2019.0078","DOIUrl":"https://doi.org/10.5423/PPJ.OA.04.2019.0078","url":null,"abstract":"Chlorine dioxide (ClO2) has been widely used as an effective disinfectant to control fungal contamination during postharvest crop storage. In this study, Fusarium oxysporum f. sp. batatas SP-f6 from the black rot symptom of sweetpotato was isolated and identified using phylogenetic analysis of elongation factor 1-α gene; we further examined the in vitro and in vivo inhibitory activities of ClO2 gas against the fungus. In the in vitro medium tests, fungal population was significantly inhibited upon increasing the concentration and exposure time. In in vivo tests, spore suspensions were drop-inoculated onto sweetpotato slices, followed by treatment using various ClO2 concentrations and treatment times to assess fungus-induced disease development in the slices. Lesion diameters decreased at the tested ClO2 concentrations over time. When sweetpotato roots were dip-inoculated in spore suspensions prior to treatment with 20 and 40 ppm of ClO2 for 0–60 min, fungal populations significantly decreased at the tested concentrations for 30–60 min. Taken together, these results showed that ClO2 gas can effectively inhibit fungal growth and disease development caused by F. oxysporum f. sp. batatas on sweetpotato. Therefore, ClO2 gas may be used as a sanitizer to control this fungus during postharvest storage of sweetpotato.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123857362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

siRNAs Derived from Cymbidium Mosaic Virus and Odontoglossum Ringspot Virus Down-modulated the Expression Levels of Endogenous Genes in Phalaenopsis equestris 蕙兰花叶病毒和牙齿舌环斑病毒sirna下调蝴蝶兰内源基因表达水平

The Plant Pathology Journal Pub Date : 2019-10-01 DOI: 10.5423/PPJ.OA.03.2019.0055

H. Lan, Cui-mei Wang, Shuang-shuang Chen, Jian-ying Zheng

{"title":"siRNAs Derived from Cymbidium Mosaic Virus and Odontoglossum Ringspot Virus Down-modulated the Expression Levels of Endogenous Genes in Phalaenopsis equestris","authors":"H. Lan, Cui-mei Wang, Shuang-shuang Chen, Jian-ying Zheng","doi":"10.5423/PPJ.OA.03.2019.0055","DOIUrl":"https://doi.org/10.5423/PPJ.OA.03.2019.0055","url":null,"abstract":"Interplay between Cymbidium mosaic virus (CymMV)/Odontoglossum ringspot virus (ORSV) and its host plant Phalaenopsis equestris remain largely unknown, which led to deficiency of effective measures to control disease of P. equestris caused by infecting viruses. In this study, for the first time, we characterized viral small interfering RNAs (vsiRNAs) profiles in P. equestris co-infected with CymMV and ORSV through small RNA sequencing technology. CymMV and ORSV small interfering RNAs (siRNAs) demonstrated several general and specific/new characteristics. vsiRNAs, with A/U bias at the first nucleotide, were predominantly 21-nt long and they were derived predominantly (90%) from viral positive-strand RNA. 21-nt siRNA duplexes with 0-nt overhangs were the most abundant 21-nt duplexes, followed by 2-nt overhangs and then 1-nt overhangs 21-nt duplexes in infected P. equestris. Continuous but heterogeneous distribution and secondary structures prediction implied that vsiRNAs originate predominantly by direct Dicer-like enzymes cleavage of imperfect duplexes in the most folded regions of the positive strand of both viruses RNA molecular. Furthermore, we totally predicted 54 target genes by vsiRNAs with psRNATarget server, including disease/stress response–related genes, RNA interference core components, cytoskeleton-related genes, photosynthesis or energy supply related genes. Gene Ontology classification showed that a majority of the predicted targets were related to cellular components and cellular processes and performed a certain function. All target genes were down-regulated with different degree by vsiRNAs as shown by real-time reverse transcription polymerase chain reaction. Taken together, CymMV and ORSV siRNAs played important roles in interplay with P. equestris by down modulating the expression levels of endogenous genes in host plant.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115360602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Inhibitory Abilities of Bacillus Isolates and Their Culture Filtrates against the Gray Mold Caused by Botrytis cinerea on Postharvest Fruit 分离芽孢杆菌及其培养滤液对果实采后灰霉病的抑制作用

The Plant Pathology Journal Pub Date : 2019-10-01 DOI: 10.5423/PPJ.OA.03.2019.0064

Xiaomeng Chen, Yajie Wang, Yu Gao, T. Gao, Dongdong Zhang

{"title":"Inhibitory Abilities of Bacillus Isolates and Their Culture Filtrates against the Gray Mold Caused by Botrytis cinerea on Postharvest Fruit","authors":"Xiaomeng Chen, Yajie Wang, Yu Gao, T. Gao, Dongdong Zhang","doi":"10.5423/PPJ.OA.03.2019.0064","DOIUrl":"https://doi.org/10.5423/PPJ.OA.03.2019.0064","url":null,"abstract":"Botrytis cinerea, a major phytopathogenic fungus, has been reported to infect more than 200 crop species worldwide, and it causes massive losses in yield. The aim of this study was to evaluate the inhibitory abilities and effects of Bacillus amyloliquefaciens RS-25, Bacillus licheniformis MG-4, Bacillus subtilis Z-14, and Bacillus subtilis Pnf-4 and their culture filtrates and extracts against the gray mold caused by B. cinerea on postharvest tomato, strawberry, and grapefruit. The results revealed that the cells of Z-14, culture filtrate of RS-25, and cells of Z-14 showed the strongest biocontrol activity against the gray mold on the strawberry, grape, and tomato fruit, respectively. All the strains produced volatile organic compounds (VOCs), and the VOCs of Pnf-4 displayed the highest inhibition values. Based on headspace solid-phase microextraction in combination with gas chromatography-mass spectrometry, esters accounted for the largest percentage of the VOCs produced by RS-25, MG-4, Z-14, and Pnf-4 (36.80%, 29.58%, 30.78%, and 36.26%, respectively). All the strains showed potent cellulase and protease activities, but no chitinase activity. RS-25, Z-14, and MG-4, but not Pnf-4, grew on chrome azurol S agar, and an orange halo was formed around the colonies. All the strains showed biofilm formation, fruit colonization, and lipopeptide production, which may be the main modes of action of the antagonists against B. cinerea on the fruit. This study provides the basis for developing natural biocontrol agents against the gray mold caused by B. cinerea on postharvest fruit.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125023912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Evolutionary Rates and Phylogeographical Analysis of Odontoglossum Ringspot Virus Based on the 166 Coat Protein Gene Sequences 基于166个外壳蛋白基因序列的牙舌环斑病毒进化速率及系统地理分析

The Plant Pathology Journal Pub Date : 2019-10-01 DOI: 10.5423/PPJ.OA.04.2019.0113

Zhen He, Tingting Dong, Weiwen Wu, Wen Chen, X. Liu, Liangjun Li

引用次数: 2

Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein 两株番茄花叶病毒新分离株全长感染克隆诱导与128-kDa蛋白两个不同氨基酸残基相关的不同症状

The Plant Pathology Journal Pub Date : 2019-10-01 DOI: 10.5423/PPJ.NT.12.2018.0286

Go-Woon Choi, J. Oh, I. Cho, H. Ju, Wen-Xing Hu, Boram Kim, E. Seo, Jong-Seok Park, L. Domier, J. Hammond, Kihak Song, Hyoun-Sub Lim

引用次数: 1

Biocontrol of Citrus Canker Disease Caused by Xanthomonas citri subsp. citri Using an Endophytic Bacillus thuringiensis 柑橘黄单胞菌溃疡病的生物防治。利用苏云金芽孢杆菌内生柑橘

The Plant Pathology Journal Pub Date : 2019-10-01 DOI: 10.5423/PPJ.OA.03.2019.0060

M. Islam, Md. Sarafat Ali, Seong-Jin Choi, J. Hyun, K. Baek

引用次数: 26

Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur 生物硫预处理对柑桔叶片黑糖的抑制作用

The Plant Pathology Journal Pub Date : 2019-10-01 DOI: 10.5423/PPJ.OA.03.2019.0067

Y. Shin, Eun Ju Ko, S. J. Kim, He Nam Hyun, Y. Jeun

{"title":"Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur","authors":"Y. Shin, Eun Ju Ko, S. J. Kim, He Nam Hyun, Y. Jeun","doi":"10.5423/PPJ.OA.03.2019.0067","DOIUrl":"https://doi.org/10.5423/PPJ.OA.03.2019.0067","url":null,"abstract":"Melanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity of bio-sulfur against D. citri was determined through in vitro experiment using artificial nutrient media. Disease severity of melanose on bio-sulfur pretreated citrus leaves was lower than that on untreated ones. To illustrate the mechanism of disease suppression by bio-sulfur, infection structures were observed with a fluorescent microscope and a scanning electron microscope. In fluorescent microscopic observation, most conidia rarely germinated. In addition, hyphal growth on leaves pretreated with bio-sulfur was inhibited compared to that on untreated ones. In scanning electron microscope images of bio-sulfur pretreated leaves, surfaces of most conidia were shrunk while hyphae were morphologically changed and frequently branched. Such microscopic observations were also found for leaves pretreated with a commercial fungicide Dithianon. These results suggest that bio-sulfur may be used to control citrus melanose as an environment friendly alternative to organic synthetic fungicides","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125186036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

An Integrated Modeling Approach for Predicting Potential Epidemics of Bacterial Blossom Blight in Kiwifruit under Climate Change 气候变化下猕猴桃细菌性花枯病流行预测的综合建模方法

The Plant Pathology Journal Pub Date : 2019-10-01 DOI: 10.5423/PPJ.OA.05.2019.0140

Kwang-Hyung Kim, Y. Koh

{"title":"An Integrated Modeling Approach for Predicting Potential Epidemics of Bacterial Blossom Blight in Kiwifruit under Climate Change","authors":"Kwang-Hyung Kim, Y. Koh","doi":"10.5423/PPJ.OA.05.2019.0140","DOIUrl":"https://doi.org/10.5423/PPJ.OA.05.2019.0140","url":null,"abstract":"The increasing variation in climatic conditions under climate change directly influences plant-microbe interactions. To account for as many variables as possible that may play critical roles in such interactions, the use of an integrated modeling approach is necessary. Here, we report for the first time a local impact assessment and adaptation study of future epidemics of kiwifruit bacterial blossom blight (KBB) in Jeonnam province, Korea, using an integrated modeling approach. This study included a series of models that integrated both the phenological responses of kiwifruit and the epidemiological responses of KBB to climatic factors with a 1 km resolution, under the RCP8.5 climate change scenario. Our results indicate that the area suitable for kiwifruit cultivation in Jeonnam province will increase and that the flowering date of kiwifruit will occur increasingly earlier, mainly due to the warming climate. Future epidemics of KBB during the predicted flowering periods were estimated using the Pss-KBB Risk Model over the predicted suitable cultivation regions, and we found location-specific, periodic outbreaks of KBB in the province through 2100. Here, we further suggest a potential, scientifically-informed, long-term adaptation strategy using a cultivar of kiwifruit with a different maturity period to relieve the pressures of future KBB risk. Our results clearly show one of the possible options for a local impact assessment and adaptation study using multiple models in an integrated way.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"26 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115206712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Survival Factor Gene FgSvf1 Is Required for Normal Growth and Stress Resistance in Fusarium graminearum 小麦赤霉病菌正常生长和抗逆性需要生存因子基因FgSvf1

The Plant Pathology Journal Pub Date : 2019-10-01 DOI: 10.5423/PPJ.OA.03.2019.0070

Taiying Li, Bok-Nam Jung, Sook-Young Park, Jungkwan Lee

{"title":"Survival Factor Gene FgSvf1 Is Required for Normal Growth and Stress Resistance in Fusarium graminearum","authors":"Taiying Li, Bok-Nam Jung, Sook-Young Park, Jungkwan Lee","doi":"10.5423/PPJ.OA.03.2019.0070","DOIUrl":"https://doi.org/10.5423/PPJ.OA.03.2019.0070","url":null,"abstract":"Survival factor 1 (Svf1) is a protein involved in cell survival pathways. In Saccharomyces cerevisiae, Svf1 is required for the diauxic growth shift and survival under stress conditions. In this study, we characterized the role of FgSvf1, the Svf1 homolog in the homothallic ascomycete fungus Fusarium graminearum. In the FgSvf1 deletion mutant, conidial germination was delayed, vegetative growth was reduced, and pathogenicity was completely abolished. Although the FgSvf1 deletion mutant produced perithecia, the normal maturation of ascospore was dismissed in deletion mutant. The FgSvf1 deletion mutant also showed reduced resistance to osmotic, fungicide, and cold stress and reduced sensitivity to oxidative stress when compared to the wild-type strain. In addition, we showed that FgSvf1 affects glycolysis, which results in the abnormal vegetative growth in the FgSvf1 deletion mutant. Further, intracellular reactive oxygen species (ROS) accumulated in the FgSvf1 deletion mutant, and this accumulated ROS might be related to the reduced sensitivity to oxidative stress and the reduced resistance to cold stress and fungicide stress. Overall, understanding the role of FgSvf1 in F. graminearum provides a new target to control F. graminearum infections in fields.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123079496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2