Quantitative plant biology最新文献

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Time-lapse confocal imaging helps to reveal a secret behind gynoecium development. 延时共聚焦成像有助于揭示雌蕊发育背后的秘密。
Quantitative plant biology Pub Date : 2025-07-18 eCollection Date: 2025-01-01 DOI: 10.1017/qpb.2025.10009
Wiktoria Wodniok
{"title":"Time-lapse confocal imaging helps to reveal a secret behind gynoecium development.","authors":"Wiktoria Wodniok","doi":"10.1017/qpb.2025.10009","DOIUrl":"10.1017/qpb.2025.10009","url":null,"abstract":"<p><p>Organ morphogenesis is a complex process and numerous factors must be considered while choosing a method for its quantitative investigation. Few methods facilitate <i>in vivo</i> imaging. These are sequential replica methods combined with scanning electron microscopy and sequential confocal microscopy imaging. The latter is now the most used method to study spatiotemporal changes of organ geometry, growth and involvement of molecular factors in regulating organ development. The time-lapse confocal imaging combined with quantitative analysis of the spatiotemporal pattern of auxin efflux proteins (PIN-FORMED) was used to investigate growth and morphogenesis of <i>Arabidopsis</i> gynoecium and enabled detailed insight into gynoecium development. Yet time-lapse imaging of the gynoecium, concealed within a flower bud, presents challenges in ensuring high-quality data during all the stages of such investigations (sample preparation, maintenance of growing organ during the relatively long time of its development, laser exposure time, etc.). Analysis of vast quantitative data was facilitated by MorphoGraphX.</p>","PeriodicalId":101358,"journal":{"name":"Quantitative plant biology","volume":"6 ","pages":"e18"},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12277207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The impact of light heterogeneity in controlled environment agriculture on biomass of microgreens. 受控环境农业光异质性对微绿生物量的影响。
Quantitative plant biology Pub Date : 2025-07-10 eCollection Date: 2025-01-01 DOI: 10.1017/qpb.2025.10003
Will Claydon, Phoebe Sutton, Ethan J Redmond, Gina Y W Vong, Alana Kluczkovski, Alice Thomas, Katherine Denby, Daphne Ezer
{"title":"The impact of light heterogeneity in controlled environment agriculture on biomass of microgreens.","authors":"Will Claydon, Phoebe Sutton, Ethan J Redmond, Gina Y W Vong, Alana Kluczkovski, Alice Thomas, Katherine Denby, Daphne Ezer","doi":"10.1017/qpb.2025.10003","DOIUrl":"10.1017/qpb.2025.10003","url":null,"abstract":"<p><p>Yield is impacted by the environmental conditions that plants are exposed to. Controlled environmental agriculture provides growers with an opportunity to fine-tune environmental conditions for optimising yield and crop quality. However, space and time constraints will limit the number of experimental conditions that can be tested, which will, in turn, limit the resolution to which environmental conditions can be optimised. Here we present an innovative experimental approach that utilises the existing heterogeneity in light quantity and quality across a vertical farm to evaluate hundreds of environmental conditions concurrently. Using an observational study design, we identify features in light quality that are most predictive of biomass in different kinds of microgreens (kale, radish and sunflower) that may inform future iterations of lighting technology development for vertical farms.</p>","PeriodicalId":101358,"journal":{"name":"Quantitative plant biology","volume":"6 ","pages":"e17"},"PeriodicalIF":0.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12277203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Participatory assessment of minor crops: A situated study on hulled wheats. 小作物参与式评价:脱壳小麦的区位研究。
Quantitative plant biology Pub Date : 2025-07-04 eCollection Date: 2025-01-01 DOI: 10.1017/qpb.2025.10010
Sofía Correa, Mathieu Thomas, Justine Lepagneul, Aubin Démonté, Pierre Berthet, Jean-Philippe Clair, Christian Dalmasso, Cédric Mary, Denis Mignard, Honorine Périno, Marie-Pierre Répécaud, Stéphane Rouvès, Léa Bernard, Gustave Fradin, Camille Vindras-Fouillet, Jean-François Vian, Olivier Hamant, Marie-Thérèse Charreyre
{"title":"Participatory assessment of minor crops: A situated study on hulled wheats.","authors":"Sofía Correa, Mathieu Thomas, Justine Lepagneul, Aubin Démonté, Pierre Berthet, Jean-Philippe Clair, Christian Dalmasso, Cédric Mary, Denis Mignard, Honorine Périno, Marie-Pierre Répécaud, Stéphane Rouvès, Léa Bernard, Gustave Fradin, Camille Vindras-Fouillet, Jean-François Vian, Olivier Hamant, Marie-Thérèse Charreyre","doi":"10.1017/qpb.2025.10010","DOIUrl":"10.1017/qpb.2025.10010","url":null,"abstract":"<p><p>Expanding crop diversity is essential to address the imminent challenges of agriculture. This is especially true for organic farming, which relies on locally adapted species and varieties. Recently, participatory research approaches have emerged as effective means to support this endeavour. In this study, we collaborated with several stakeholders in the Lyon region, France, to evaluate three minor species related to common wheat (<i>Triticum aestivum</i> subsp<i>. aestivum</i>): einkorn (<i>Triticum monococcum</i> subsp<i>. monococcum</i>), emmer (<i>Triticum turgidum</i> subsp<i>. dicoccum</i>) and spelt (<i>Triticum aestivum</i> subsp. <i>spelta</i> (L.) Thell). First, we assessed the agronomic characteristics of each species, highlighting a distinction of einkorn that was associated with high tillering, high protein content, a long phenological cycle, small kernels and low relative yields. Second, we compared intra-species variabilities, revealing greater variation in emmer and spelt. Lastly, outcomes of the participatory approach, including testing adaptive methods and fostering collective learning, may interest other participatory research groups.</p>","PeriodicalId":101358,"journal":{"name":"Quantitative plant biology","volume":"6 ","pages":"e21"},"PeriodicalIF":0.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12277212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An integrative process-based model of fruit growth as a function of carbon and water fluxes modulated by endogenous abscisic acid in blueberry fruit. 内源脱落酸调节蓝莓果实碳和水通量的综合过程模型
Quantitative plant biology Pub Date : 2025-06-30 eCollection Date: 2025-01-01 DOI: 10.1017/qpb.2025.10011
Sun Woo Chung, Kyungdahm Yun, Soo-Hyung Kim
{"title":"An integrative process-based model of fruit growth as a function of carbon and water fluxes modulated by endogenous abscisic acid in blueberry fruit.","authors":"Sun Woo Chung, Kyungdahm Yun, Soo-Hyung Kim","doi":"10.1017/qpb.2025.10011","DOIUrl":"10.1017/qpb.2025.10011","url":null,"abstract":"<p><p>Fruit growth is driven by the interaction of environmental cues and phytohormonal signals. Biophysical models have captured the general trend of fruit growth but often overlook the regulatory role of phytohormones. This study integrates a biophysical framework with the quantitative response of endogenous abscisic acid (ABA) in fruit. ABA dynamics are incorporated as a ripening signal, influencing sugar uptake, respiration, hydraulic conductance and transpiration processes. The model has been primarily tested on blueberries, a fruit with well-characterised ABA responses. Simulations show predictive accuracy and explanatory capability for fruit mass under variable climatic conditions. Notably, the model effectively simulates the impacts of environmental stresses such as heat, cold and drought, capturing the resulting physiological delays in fruit growth. Our research underscores the potential of integrating phytohormonal responses into biophysical models, providing key insights into fruit growth dynamics and practical guidance for optimising crop management under increasing climate uncertainties.</p>","PeriodicalId":101358,"journal":{"name":"Quantitative plant biology","volume":"6 ","pages":"e19"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12277213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chloride transport and homeostasis in plants. 植物中氯化物的运输和体内平衡。
Quantitative plant biology Pub Date : 2025-06-30 eCollection Date: 2025-01-01 DOI: 10.1017/qpb.2025.10008
Harverth Silva-Herrera, Stefanie Wege, Bastian Leander Franzisky, Namrah Ahmad, M Rob G Roelfsema, Christoph-Martin Geilfus
{"title":"Chloride transport and homeostasis in plants.","authors":"Harverth Silva-Herrera, Stefanie Wege, Bastian Leander Franzisky, Namrah Ahmad, M Rob G Roelfsema, Christoph-Martin Geilfus","doi":"10.1017/qpb.2025.10008","DOIUrl":"10.1017/qpb.2025.10008","url":null,"abstract":"<p><p>The micronutrient chloride (Cl<sup>-</sup>) plays key roles in plant physiology, from photosystem II and vacuolar ATPase activity to osmoregulation, turgor maintenance and drought resilience, while also posing toxicity risks at high concentrations. This review examines Cl<sup>-</sup> uptake, transport and homeostasis, focussing on adaptations balancing its dual roles as a nutrient and toxicant. Key transporters, including NPF, SLAH, ALMT, CLC and CCC families, mediate Cl<sup>-</sup> fluxes to maintain ionic balance and prevent toxicity. Plants employ strategies such as selective uptake and vacuolar compartmentalization to cope with high salinity. Cl<sup>-</sup> also influences nitrogen-use efficiency and plant productivity. Advances in transporter biology reveal the role of Cl<sup>-</sup> in water-use efficiency, drought resilience and stress adaptation.</p>","PeriodicalId":101358,"journal":{"name":"Quantitative plant biology","volume":"6 ","pages":"e20"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12277217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Manganese handling in plants: Advances in the mechanistic and functional understanding of transport pathways. 锰在植物中的处理:运输途径的机制和功能理解的进展。
Quantitative plant biology Pub Date : 2025-06-20 eCollection Date: 2025-01-01 DOI: 10.1017/qpb.2025.10012
Bastian Meier, Oriana Mariani, Edgar Peiter
{"title":"Manganese handling in plants: Advances in the mechanistic and functional understanding of transport pathways.","authors":"Bastian Meier, Oriana Mariani, Edgar Peiter","doi":"10.1017/qpb.2025.10012","DOIUrl":"10.1017/qpb.2025.10012","url":null,"abstract":"<p><p>As the catalytic centre of the oxygen-evolving complex in photosystem II and a co-factor of glycosyltransferases and many other proteins, manganese (Mn) is essential for plants and a limiting factor for crop production. However, an excessive Mn availability is toxic to plants. Therefore, mechanisms need to be in place to maintain Mn homeostasis under fluctuating Mn availability. This review summarises our current understanding of the mechanisms that move Mn from the soil to its cellular targets and maintain Mn homeostasis. We zoom in from the whole-plant perspective to the intracellular allocation of the metal by transport proteins of different families acting in concert. In particular, organellar Mn supply by members of the recently identified bivalent cation transporter family and the post-translational regulation of Mn transporters by calcium-regulated phosphorylation have been a focus of current research. Finally, the emergent diversity of Mn handling beyond the Arabidopsis model will be addressed.</p>","PeriodicalId":101358,"journal":{"name":"Quantitative plant biology","volume":"6 ","pages":"e16"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Four-dimensional phenotyping reveals MYOSIN XI-dependent establishment of branch morphology through upward- and stably-directed growth in Arabidopsis. 四维表型显示MYOSIN xi依赖于拟南芥通过向上和稳定的定向生长建立分支形态。
Quantitative plant biology Pub Date : 2025-06-10 eCollection Date: 2025-01-01 DOI: 10.1017/qpb.2025.10007
Daichi Yoshida, Itsuki Kunita, Masashi Toda, Haruko Ueda, Takumi Higaki
{"title":"Four-dimensional phenotyping reveals MYOSIN XI-dependent establishment of branch morphology through upward- and stably-directed growth in Arabidopsis.","authors":"Daichi Yoshida, Itsuki Kunita, Masashi Toda, Haruko Ueda, Takumi Higaki","doi":"10.1017/qpb.2025.10007","DOIUrl":"10.1017/qpb.2025.10007","url":null,"abstract":"<p><p>Plants develop characteristic shoot architectures by extending branches at specific angles. Primary shoots bend in response to gravity and then adjust the orientation through an organ-straightening process to achieve a mechanically favorable shape. However, how plants integrate branch structure with the shoot architecture remains uncertain. Here, we examined the lateral branch morphology of <i>Arabidopsis thaliana</i> mutants for myosin XI motor proteins through a combination of three-dimensional reconstruction and temporal imaging. The wild type and <i>myosin xif</i> mutant formed S-shaped branches and gradually adjusted the branch angle upwards. The <i>myosin xik</i> mutant exhibited straighter and drooping branches and maintained branch angles. The <i>myosin xif xik</i> double mutant formed branches with irregular directional changes with fluctuating angles. These results suggest that MYOSIN XIk and XIf are required for the establishment of branch morphology through upward bending, stabilizing growth direction, and maintaining curvature.</p>","PeriodicalId":101358,"journal":{"name":"Quantitative plant biology","volume":"6 ","pages":"e15"},"PeriodicalIF":0.0,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186554/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Untangling iron threads: A deep dive into plant intracellular pools. 解开缠结的铁丝:深入植物细胞内池。
Quantitative plant biology Pub Date : 2025-06-09 eCollection Date: 2025-01-01 DOI: 10.1017/qpb.2025.11
Alexandra Leskova, Tou C Xiong, Stéphane Mari, Catherine Curie
{"title":"Untangling iron threads: A deep dive into plant intracellular pools.","authors":"Alexandra Leskova, Tou C Xiong, Stéphane Mari, Catherine Curie","doi":"10.1017/qpb.2025.11","DOIUrl":"10.1017/qpb.2025.11","url":null,"abstract":"<p><p>Iron (Fe) is an essential element in plants, involved in numerous metabolic processes including photosynthesis. Its cellular concentration must be regulated accurately to avoid toxicity while meeting metabolic demands. This review explores the distribution, dynamics, and regulation of Fe pools in plant cells, focusing on recent advances in imaging and quantification techniques. We discuss the major Fe compartments-chloroplasts, vacuoles, apoplasts-and their interaction to maintain Fe homeostasis, as well as novel methodologies like single-cell ICP-MS that have transformed our understanding of Fe localization. By summarizing the current knowledge on intracellular Fe dynamics and the complex interplay between different Fe pools, we provide insights into the mechanisms that underpin Fe regulation in plants, which is crucial for future breeding programs aimed at improving plant resilience and nutritional quality.</p>","PeriodicalId":101358,"journal":{"name":"Quantitative plant biology","volume":"6 ","pages":"e14"},"PeriodicalIF":0.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potassium homeostasis and signalling: from the whole plant to the subcellular level. 钾稳态和信号传导:从整个植物到亚细胞水平。
Quantitative plant biology Pub Date : 2025-05-08 eCollection Date: 2025-01-01 DOI: 10.1017/qpb.2025.10
Lars H Wegner, Igor Pottosin, Ingo Dreyer, Sergey Shabala
{"title":"Potassium homeostasis and signalling: from the whole plant to the subcellular level.","authors":"Lars H Wegner, Igor Pottosin, Ingo Dreyer, Sergey Shabala","doi":"10.1017/qpb.2025.10","DOIUrl":"10.1017/qpb.2025.10","url":null,"abstract":"<p><p>Potassium is an essential macronutrient required for plant growth and development. Over the recent decade, an important signalling role of K<sup>+</sup> has emerged. Here, we discuss some aspects of such signalling at the various levels of plant functional organisation. The topic covered include: (1) mechanisms of long-distant K<sup>+</sup> transport in the xylem and phloem and the molecular identity and regulation of K<sup>+</sup> loading and unloading into plant vasculature; (2) essentiality and physiological roles of K<sup>+</sup> cycling between shoots and roots; (3) plant sensing and signalling of low K<sup>+</sup>; (4) maintenance of K<sup>+</sup> homeostasis at the cellular level; (5) stress-induced modulation of cytosolic K<sup>+</sup> as a signal in plant adaptive responses to hostile environment; (6) stress-specific K<sup>+</sup> \"signatures\" and mechanisms of their decoding by regulation of purine metabolism and H<sup>+</sup>-ATPase activity; (7) cytosolic K<sup>+</sup> loss as a metabolic switch and a regulator of autophagy; and (8) vacuolar K<sup>+</sup> transport and sensing.</p>","PeriodicalId":101358,"journal":{"name":"Quantitative plant biology","volume":"6 ","pages":"e13"},"PeriodicalIF":0.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12086022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144103405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
To flow or to grow? Impacts of tapping on sugar maple. 流动还是生长?攻丝对糖枫的影响。
Quantitative plant biology Pub Date : 2025-04-07 eCollection Date: 2025-01-01 DOI: 10.1017/qpb.2025.9
Hannah Grace McNulty, Roberto Silvestro, Minhui He, Fabio Gennaretti, Sergio Rossi
{"title":"To flow or to grow? Impacts of tapping on sugar maple.","authors":"Hannah Grace McNulty, Roberto Silvestro, Minhui He, Fabio Gennaretti, Sergio Rossi","doi":"10.1017/qpb.2025.9","DOIUrl":"https://doi.org/10.1017/qpb.2025.9","url":null,"abstract":"<p><p>Maple sugaring is a rapidly growing industry in North America. Maples are tapped annually, thus undergoing repeated wounding and resource reduction for sap water collection. We aim to understand the effects of tapping and sap exudation on annual radial wood growth and xylem traits in sugar maple (<i>Acer saccharum</i> Marsh.), utilizing eight mature trees monitored during 2018-2021 in Simoncouche, Canada. Compared to the first year of tapping, trees exhibited a 49.7% drop in tree-ring width. Vessel density, potential hydraulic conductivity and hydraulic vessel diameter decreased, but not lumen area. We showed evidence of a trade-off among sap extraction, resource depletion and reduced tree growth. The repeated reduction of resources through tapping can have a detrimental effect on tree growth, even if the effect on the hydraulic function remains marginal. These insights underscore the need for sustainable tapping practices that consider the long-term health and productivity of sugar maple trees.</p>","PeriodicalId":101358,"journal":{"name":"Quantitative plant biology","volume":"6 ","pages":"e11"},"PeriodicalIF":0.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12035781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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