Mutation Research Letters最新文献_第9页

The induction of dominant somatic mutations at the Dlb-1 locus 在Dlb-1位点诱导显性体细胞突变

Mutation Research Letters Pub Date : 1995-02-01 DOI: 10.1016/0165-7992(95)90059-4

Lidia Cosentino, John A. Heddle

引用次数: 7

G2 phase repair of X-ray-induced chromosomal DNA damage in trichothiodystrophy cells x射线诱导毛硫营养不良细胞染色体DNA损伤的G2期修复

Mutation Research Letters Pub Date : 1995-02-01 DOI: 10.1016/0165-7992(95)90058-6

Katherine K. Sanford , Ram Parshad , Floyd M. Price , Robert E. Tarone , Alan R. Lehmann

{"title":"G2 phase repair of X-ray-induced chromosomal DNA damage in trichothiodystrophy cells","authors":"Katherine K. Sanford , Ram Parshad , Floyd M. Price , Robert E. Tarone , Alan R. Lehmann","doi":"10.1016/0165-7992(95)90058-6","DOIUrl":"10.1016/0165-7992(95)90058-6","url":null,"abstract":"<div>The repair of X-ray-induced DNA damage during G2 cell-cycle phase has been examined in lines of skin fibroblasts from three patients with trichothiodystrophy (TTD), one with apparently normal and two with defective nucleotide excision repair (NER). These responses are compared with those of five lines from clinically normal controls, lines from xeroderma pigmentosum (XP), Cockayne syndrome (CS), Down syndrome (DS), and ataxia telangiectasia (AT) patients. Chromosomal DNA repair was measured as the chromatid aberration frequency (CAF) or total number of chromatid breaks and long gaps per 100 metaphase cells, determined 0.5–1.5 h after X-irradiation (53 rad). Chromatid breaks and gaps (as defined herein) represent unrepaired DNA strand breaks. Only one of the TTD lines, TTD 1BR, showed an abnormally high CAF. This line was shown subsequently to be of a different complementation group, representing a new nucleotide excision repair gene. An abnormally high CAF was also observed, as reported previously, in XP-C, AT and DS but not in CS skin fibroblasts. In addition, cell lines were examined for DNA incision activity by an indirect method in which chromatid aberrations were enumerated with or without ara-C, an inhibitor of repair synthesis, added after X-irradiation. All TTD lines had abnormally low incision activity.</div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"346 2","pages":"Pages 107-114"},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90058-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18885060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Mathematical parameters for quantification of mutational responses in bacteria 定量细菌突变反应的数学参数

Mutation Research Letters Pub Date : 1995-02-01 DOI: 10.1016/0165-7992(95)90054-3

Teresa Roldán-Arjona , Carmen Pueyo , Robert H.Haynes

引用次数: 4

The relative radiosensitivity of TK6 and WI-L2-NS lymphoblastoid cells derived from a common source is primarily determined by their p53 mutational status 来自同一来源的TK6和WI-L2-NS淋巴母细胞的相对放射敏感性主要取决于它们的p53突变状态

Mutation Research Letters Pub Date : 1995-02-01 DOI: 10.1016/0165-7992(95)90055-1

W. Zhen , C.M. Denault , K. Loviscek , S. Walter , L Geng , A.T.M. Vaughan

引用次数: 66

Use of three-way differential staining and liquid holding for the assessment of individual repair capacity 使用三向鉴别染色和液体持有的个人修复能力的评估

Mutation Research Letters Pub Date : 1995-02-01 DOI: 10.1016/0165-7992(95)90056-X

Isabella Ponzanelli, Stefano Landi, Roberto Barale

引用次数: 3

Effect of carboxymethyl-chitin-glucan on cyclophosphamide induced mutagenicity 羧甲基几丁质葡聚糖对环磷酰胺致突变性的影响

Mutation Research Letters Pub Date : 1995-01-01 DOI: 10.1016/0165-7992(95)90067-5

Darina Chorvatovičová , Josef Šandula

引用次数: 31

Inducible stable DNA replication of Escherichia coli tolerates unexcised pyrimidine dimers in an uvr-dependent manner 诱导稳定的DNA复制大肠杆菌耐受未切除的嘧啶二聚体以紫外线依赖的方式

Mutation Research Letters Pub Date : 1995-01-01 DOI: 10.1016/0165-7992(95)90062-4

Milena Sedliaková, Viera Slezáriková, Frantisšek Miroslav Piršel

引用次数: 3

Effect of cigarette smoke on the mutagenic activation of various carcinogens in hamster 香烟烟雾对仓鼠多种致癌物致突变激活的影响

Mutation Research Letters Pub Date : 1995-01-01 DOI: 10.1016/0165-7992(95)90061-6

Yukio Mori , Kazunori Iimura , Fumio Furukawa , Akiyoshi Nishikawa , Michihito Takahashi , Yoichi Konishi

{"title":"Effect of cigarette smoke on the mutagenic activation of various carcinogens in hamster","authors":"Yukio Mori , Kazunori Iimura , Fumio Furukawa , Akiyoshi Nishikawa , Michihito Takahashi , Yoichi Konishi","doi":"10.1016/0165-7992(95)90061-6","DOIUrl":"10.1016/0165-7992(95)90061-6","url":null,"abstract":"<div>Male Syrian golden hamsters were exposed for 1 or 2 weeks to smoke produced by commercial non-filter cigarettes for 5 consecutive days in a Hamburg type II smoking machine. Postmitochondrial fractions (S9) prepared from the liver, lungs, and pancreas were used in the Ames liquid incubation assay, in order to assess the effect of cigarette smoke (CS) on the metabolic activation of four groups of procarcinogens. The mutagenic activities of five heterocyclic amines on strain TA98 in the presence of liver S9 mix were induced up to 3.7 times above controls including sham smoke control, while no significant alteration of mutagenicity was observed with 3′-hydroxymethyl-N,N-dimethyl-4-aminoazobenzene and benzo[a]pyrene on TA98 or with N-nirosobis(2-oxopropyl)amine (BOP) on TA100. A similar stimulation of metabolic activation was also observed for 3-amino-1,4-dimethyl-5H-pyridol[4,3,-b]indole (Trp-P-1) with S9 from the lungs but not from the pancreas. The mutagenic potential of 11 carcinogens including aflatoxin B1 (AFB1) and two other heterocyclic amines was also examined using liver S9 from male hamsters pretreated with phenobarbital (PB) or 3-methylcholanthrene (MC). The numbers of revertant colonies were much higher (2-20-fold) in the presence of MC-treated liver S9 than in the presence of PB-treated liver S9, except in the case of AFB1 which showed a higher mutagenicity with PB-induced S9. 7,8-Benzoflavone considerably inhibited the activities of 2-amino-3-3-methylimidazo[4,5-f]quinoline (IQ) and Trp-P-1 in the presence of either untreated, MC- or CS-treated liver S9, whereas metyrapone was totally lacking this effect, indicating that cy</div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"346 1","pages":"Pages 1-8"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90061-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18538824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Genotoxic effects of chemicals in the single cell gel (SCG) test with human blood cells in relation to the induction of sister-chromatid exchanges (SCE) 单细胞凝胶(SCG)试验中化学物质对人血细胞的基因毒性作用与诱导姐妹染色单体交换(SCE)的关系

Mutation Research Letters Pub Date : 1995-01-01 DOI: 10.1016/0165-7992(95)90068-3

Andreas Hartmann, Günter Speit

{"title":"Genotoxic effects of chemicals in the single cell gel (SCG) test with human blood cells in relation to the induction of sister-chromatid exchanges (SCE)","authors":"Andreas Hartmann, Günter Speit","doi":"10.1016/0165-7992(95)90068-3","DOIUrl":"10.1016/0165-7992(95)90068-3","url":null,"abstract":"<div>In a comparative study, henzo[a]pyrene (BaP), cyclophosphamide (CP), N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) and tetrachloroethylene (PER) were tested for their ability to induce genotoxic effects in the single cell gel (SCG) test and the sister-chromatid exchange (SCE) test with human blood cells. MNNG as well as S9 mix activated BaP- and CP-induced DNA effects in both tests in a dose-dependent manner. While the range of concentrations which induced DNA migration or SCE was the same for MNNG and for Bap, much higher CP concentrations were necessary for a positive response in the SCG test than in the SCE test. PER was tested in the absence and in the presence of S9 mix and neither induced DNA migration nor increased SCE frequencies. In these experiments, a clear cytotoxic effect of PER was observed. To investigate a possible influence of DNA repair on the effects in the SCG test, cells were treated for 2 h and further incubated for 1 h after removal of the test substance. This procedure caused a clear decrease in induced DNA migration in experiments with Bap and CP, whereas no reduction was found with MNNG. This modified protocol did not lead to the detection of DNA effects after treatment with PER. The results indicate that the SCG test responds to various DNA lesions and does not seem to be sensitive to non-genotoxic cell killing. Its sensitivity obviously depends on the type(s) of induced DNA lesions and the effects can be modified by DNA repair processes in a complex manner. For the detection of genotoxic properties of chemicals with the in vitro SCG test, a single evaluation at the end of the exposure period seems to be sufficient.</div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"346 1","pages":"Pages 49-56"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90068-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18538831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 121

Evidence for the protective effect of ascorbic acid (vitamin C) in treatment with γ-rays and chromium (VI) oxide (CrO3) in somatic cells of Drosophila 抗坏血酸（维生素C）对果蝇体细胞γ射线和氧化铬（CrO3）保护作用的证据

Mutation Research Letters Pub Date : 1995-01-01 DOI: 10.1016/0165-7992(95)90064-0

O. Olvera , S. Zimmering , C. Arceo , J. Guzman , M.E. de la Rosa

引用次数: 16