Mutation Research Letters最新文献_第5页

Study on the antimutagenic effect of pine needle extract 松针提取物的抗诱变作用研究

Mutation Research Letters Pub Date : 1995-08-01 DOI: 10.1016/0165-7992(95)00026-7

Zhiming Kong , Zhengtao Liu , Bangyu Ding

引用次数: 29

A comparative study of TK6 human lymphoblastoid and L5178Y mouse lymphoma cell lines in the in vitro micronucleus test TK6人淋巴母细胞样细胞与L5178Y小鼠淋巴瘤细胞系体外微核试验的比较研究

Mutation Research Letters Pub Date : 1995-08-01 DOI: 10.1016/0165-7992(95)00027-5

Li-Shi Zhang , Masamitsu Honma, Makoto Hayahshi, Takayoshi Suzuki, Atsuko Matsuoka, Toshio Sofuni

引用次数: 52

Micronucleus assay in pulmonary alveolar macrophages, a simple model to detect genotoxicity of environmental agents entering through the inhalation route 肺泡巨噬细胞微核测定，一种检测环境物质经吸入途径进入的遗传毒性的简单模型

Mutation Research Letters Pub Date : 1995-07-01 DOI: 10.1016/0165-7992(95)90071-3

K. Sahu, R.K. Das

引用次数: 3

The transmission of FISH-painted patterns derived from complex chromosome exchanges fish彩绘图案的传播源于复杂的染色体交换

Mutation Research Letters Pub Date : 1995-07-01 DOI: 10.1016/0165-7992(95)90075-6

John R.K. Savage

引用次数: 32

An investigation of the cytotoxic and mutagenic potential of low intensity laser irradiation in Friend erythroleukaemia cells 低强度激光照射对Friend红白血病细胞的细胞毒性和致突变潜能的研究

Mutation Research Letters Pub Date : 1995-07-01 DOI: 10.1016/0165-7992(95)90072-1

I.D. Logan , P.G. McKenna , Y.A. Barnett

{"title":"An investigation of the cytotoxic and mutagenic potential of low intensity laser irradiation in Friend erythroleukaemia cells","authors":"I.D. Logan , P.G. McKenna , Y.A. Barnett","doi":"10.1016/0165-7992(95)90072-1","DOIUrl":"10.1016/0165-7992(95)90072-1","url":null,"abstract":"<div><p>The purpose of this study was to investigate the cytotoxic and genotoxic potential of low intensity laser irradiation (660 nm, 12 mW, 5 kHz) on mammalian cells. Thymidine kinase (TK)-positive and TK-deficient Friend erythroleukaemia (FEL) cells, clone 707 and subclone 707BUF respectively, were used in this investigation. Following irradiation of exponentially growing cells in suspension at doses of 2 and 20 J/cm<sup>2</sup> a number of sensitive bioassays were used to facilitate the detection of laser-induced mutations, DNA damage and cell killing. Mutations were assessed by the examination of chromosome spreads, the determination of micronucleus frequency and by the determination of the mutant frequency at the <em>hypoxanthine-guanine phosphoribosyltransferase (hgprt)</em> locus. DNA damage was quantified using a sensitive ELISA. The cytotoxic effect of laser irradiation was assessed using a cloning assay. The results of this investigation did not show any significant increase in mutation frequency, DNA damage or cell survival in the laser-irradiated cells, compared to sham-irradiated controls. The lack of any demonstrable cytotoxic and genotoxic effects of low intensity laser irradiation on mammalian cells in culture would support it as being a safe modality for clinical use.</p></div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"347 2","pages":"Pages 67-71"},"PeriodicalIF":0.0,"publicationDate":"1995-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90072-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18655309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

XPG protein has a structure-specific endonuclease activity XPG蛋白具有结构特异性内切酶活性

Mutation Research Letters Pub Date : 1995-07-01 DOI: 10.1016/0165-7992(95)90070-5

Kieran G. Cloud, Binghui Shen, Gary F. Strniste, Min S. Park

引用次数: 37

Sample size for the estimation of polychromatic to normochromatic erythrocyte ratio in the bone marrow micronucleus test 骨髓微核试验中估计多染红细胞与正染红细胞比例的样本量

Mutation Research Letters Pub Date : 1995-07-01 DOI: 10.1016/0165-7992(95)90076-4

B. Bhaskar Gollapudi, Lisa G. McFadden

引用次数: 78

The suitability of rat peripheral blood in suchronic studies for the micronucleus assay 大鼠外周血用于微核试验的适宜性

Mutation Research Letters Pub Date : 1995-07-01 DOI: 10.1016/0165-7992(95)90073-X

Shougo Asanami, Kazuyuki Shimono, Osamu Sawamoto, Kazunobu Kurisu, Motoo Uejima

{"title":"The suitability of rat peripheral blood in suchronic studies for the micronucleus assay","authors":"Shougo Asanami, Kazuyuki Shimono, Osamu Sawamoto, Kazunobu Kurisu, Motoo Uejima","doi":"10.1016/0165-7992(95)90073-X","DOIUrl":"https://doi.org/10.1016/0165-7992(95)90073-X","url":null,"abstract":"<div><p>To examine the suitability of using rat peripheral blood from animals used in subchronic toxicity studies for micronucleus analysis, we orally administered phenacetin or 6-mercaptopurine for 14 days to groups of six rats and compared their micronucleus frequencies to the bone marrow micronucleus frequencies of rats similarly treated for only 2 days. In the 14-day test, phenacetin significantly increased the frequency of micronucleated reticulocytes in peripheral blood at 500 mg/kg starting from day 9, and at 750 and 1500 mg/kg starting from day 6; 6-mercaptopurine topurine gave a positive response at 20 mg/kg starting from day 6. Positive responses in the bone marrow assay were obtained at the same dose levels. In the 2-day test, micronucleated polychromatic erythrocyte frequencies increased significantly at 1000 and 2000 mg/kg for phenacetin, and at 50, 100, and 200 mg/kg for 6-mercaptopurine. These results suggest that micronucleus assays using peripheral blood from rats in subchronic animal studies of phenacetin and 6-mercaptopurine are feasible and at least as sensitive for the assessment of micronuclei as an acute bone marrow micronucleus test.</p></div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"347 2","pages":"Pages 73-78"},"PeriodicalIF":0.0,"publicationDate":"1995-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90073-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72106908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Streptozotocin-induced genotoxic effects in Chinese hamster cells: The resistant phenotype of V79 cells 链脲佐菌素对中国仓鼠细胞的基因毒性作用:V79细胞的抗性表型

Mutation Research Letters Pub Date : 1995-07-01 DOI: 10.1016/0165-7992(95)90074-8

M.S. Capucci , M.E. Hoffmann , A.T. Natarajan

引用次数: 11

Clastogenicity of isoamylene oxide to rat lymphocytes in culture 氧化异丙烯对培养大鼠淋巴细胞的致裂性

Mutation Research Letters Pub Date : 1995-06-01 DOI: 10.1016/0165-7992(95)90025-X

B.Bhaskar Gollapudi , V.Ann Linscombe , J.W. Wilmer

{"title":"Clastogenicity of isoamylene oxide to rat lymphocytes in culture","authors":"B.Bhaskar Gollapudi , V.Ann Linscombe , J.W. Wilmer","doi":"10.1016/0165-7992(95)90025-X","DOIUrl":"10.1016/0165-7992(95)90025-X","url":null,"abstract":"<div><p>The mutagenic activity of the aliphatic epoxide isoamylene oxide (2-methyl-2,3-epoxybutane) is not readily detectable in the standard Ames test. In this study, the clastogenic potential of isoamylene oxide was evaluated using an in vitro mammalian cell culture system. Approximately 48 h after establishing primary cultures of rat lymphoocyte cultures, the cells were treated for 4 h with various concentrations of isoamylene oxide (50, 166.7, 500, 1666.7 and 5000 μg/ml in the initial assay and 500, 1000, 2000, 3000, 4000, and 5000 μg/ml in the confirmatory assay). The cultures were harvested 24 h after termination of the treatment. Based upon the mitotic indices, cultures treated with the three highest concentrations in both the initial and confirmatory assays were evaluated to estimate the chromosomal aberration frequencies. Isoamylene oxide demonstrated a strong clastogenic activity in this assay: up to 29% aberrant cells (without gaps) were observed at the highest concentration analyzed. The presence of an external metabolic activation system (S9) did not seem to influence the magnitude of the response at the dose levels analyzed.</p></div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"347 1","pages":"Pages 9-12"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90025-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18602924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2