Journal of EuCornea最新文献

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In vitro anti-angiogenic effects of cryo-preserved amniotic membrane and the role of TIMP2 and thrombospondin 低温保存羊膜体外抗血管生成作用及TIMP2和血栓反应蛋白的作用

Journal of EuCornea Pub Date : 2018-06-01 DOI: 10.1016/j.xjec.2017.09.001

Lana Akram Faraj , Elizabeth Anne Stewart , Réka Albert , Claire Louise Allen , Goran Petrovski , Harminder Singh Dua , Winfried M.K. Amoaku

{"title":"In vitro anti-angiogenic effects of cryo-preserved amniotic membrane and the role of TIMP2 and thrombospondin","authors":"Lana Akram Faraj , Elizabeth Anne Stewart , Réka Albert , Claire Louise Allen , Goran Petrovski , Harminder Singh Dua , Winfried M.K. Amoaku","doi":"10.1016/j.xjec.2017.09.001","DOIUrl":"10.1016/j.xjec.2017.09.001","url":null,"abstract":"<div><h3>Background</h3><p>Amniotic membrane (AM) has been used as a biological substrate in ophthalmology and other fields for several years. It has a valuable role in ocular surface burns and non-healing ulcers. It is known that AM is non-immunogenic and has anti-inflammatory and anti-fibrotic properties and supports epithelial cell proliferation and migration. These are supposedly mediated through a host of growth factors and cytokines. Despite its clinically accepted role in reducing corneal vascularisation when applied on the eye, there have been contradicting reports on the effect of AM on angiogenesis.</p></div><div><h3>Methods</h3><p>The effect of soluble factors released from clinically prepared cryo-preserved AM on cultured human umbilical vein endothelial cell (HUVEC) proliferation was quantified and angiogenesis assessed on matrigel. Antibody blockage of soluble tissue inhibitors of metalloproteinase (TIMP)2 and Thrombospondin, identified on Searchlight protein arrays, was used to evaluate their role in the effect of cryo-preserved AM.</p></div><div><h3>Results</h3><p>AM conditioned medium (AMCM) was found to reduce HUVEC proliferation and angiogenesis. Angiogenesis was reduced by direct application of AM, a gradient effect was observed, where HUVEC closer to the AM failed completely in forming any tubules. In addition, HUVEC failed to survive directly on the AM. Analysis of the soluble factors released by the amnion included high levels of anti-angiogenic factors, thrombospondin and TIMP 1 and 2. Antibody blockage of TIMP2 and thrombospondin prevented the anti-angiogenic effect of AMCM and AM.</p></div><div><h3>Conclusion</h3><p>AM <em>in vitro</em> has potent anti-angiogenic properties dependent on secreted TIMP2 and thrombospondin, validating some of the effects observed clinically.</p></div>","PeriodicalId":100782,"journal":{"name":"Journal of EuCornea","volume":"1 1","pages":"Pages 3-7"},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.xjec.2017.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90910607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

A rare case of delayed post-operative migration of intracameral perfluoropropane into pre-descemet space causing a re-detachment of descemet membrane 一个罕见的病例延迟手术后迁移的内窥镜全氟丙烷到前网膜间隙导致网膜膜的重新脱离

Journal of EuCornea Pub Date : 2018-06-01 DOI: 10.1016/j.xjec.2018.02.001

Avnindra Gupta, Shilpi Diwan, T. Bharat Ratna, Rashmi Deshmukh

{"title":"A rare case of delayed post-operative migration of intracameral perfluoropropane into pre-descemet space causing a re-detachment of descemet membrane","authors":"Avnindra Gupta, Shilpi Diwan, T. Bharat Ratna, Rashmi Deshmukh","doi":"10.1016/j.xjec.2018.02.001","DOIUrl":"10.1016/j.xjec.2018.02.001","url":null,"abstract":"<div><h3>Purpose</h3><p>To report a case of re-detachment of descemet membrane subsequent to delayed post-operative migration of intracameral perfluoropropane (C3F8) into the pre-descemet space.</p></div><div><h3>Methods</h3><p>A post-phacoemulsification descemet membrane detachment (DMD) was treated by performing descemetopexy using intracameral C3F8 (14%). The descemet membrane was attached on the first post-operative day and there was a single intracameral gas bubble. However, at one week, there was a second gas bubble noted in the pre-descemet space and the descemet membrane had detached again. A stromal vent incision was used to release the C3F8 gas in pre-descemet space and intracameral C3F8 was injected again to facilitate attachment.</p></div><div><h3>Result</h3><p>Slit lamp evaluation revealed an attached descemet membrane on the first day following the stromal vent incision with repeat intracameral gas injection. At one week, the corneal edema cleared and best-corrected visual acuity (BCVA) improved to 20/40.</p></div><div><h3>Conclusion</h3><p>Delayed post-operative migration of intracameral C3F8 may occur causing a re-detachment of the descemet membrane. Stromal vent incisions can be used to drain pre-descemet gas bubble and re-injection of C3F8 intracamerally to facilitate re-attachment.</p></div>","PeriodicalId":100782,"journal":{"name":"Journal of EuCornea","volume":"1 1","pages":"Pages 19-21"},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.xjec.2018.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74839932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corneal incision width after lens implantation: Comparing delivery systems 晶状体植入术后角膜切口宽度:比较输送系统

Journal of EuCornea Pub Date : 2018-06-01 DOI: 10.1016/j.xjec.2018.04.001

Carlo Cagini , Maria Teresa Perri , Anna Pia Di Napoli , Tito Fiore , Marco Messina , Leopoldo Spadea , Gian Maria Cavallini

{"title":"Corneal incision width after lens implantation: Comparing delivery systems","authors":"Carlo Cagini , Maria Teresa Perri , Anna Pia Di Napoli , Tito Fiore , Marco Messina , Leopoldo Spadea , Gian Maria Cavallini","doi":"10.1016/j.xjec.2018.04.001","DOIUrl":"10.1016/j.xjec.2018.04.001","url":null,"abstract":"<div><h3>Purpose</h3><p>To compare corneal incision width after phacoemulsification and intraocular lens implantation (IOL) using different delivery systems.</p></div><div><h3>Methods</h3><p>One hundred and seventeen patients with cataract and no other anterior segment pathological features or previous eye surgery underwent cataract surgery with IOL implantation through a 2.2 mm incision. Three foldable IOL were implanted with their recommended delivery systems: Acrysof© SN60WF with Monarch© III/cartridge D (Group A, 38 patients); Tecnis© ZCB00 with Unfolder Platinum/cartridge easy load (Group B, 38 patients); Acrysof© SN60WF with Ultrasert™ preloaded system (Group C, 42 patients). Incision width was measured before and after phacoemulsification and IOL implantation.</p></div><div><h3>Results</h3><p>Before and after phacoemulsification incision width was, respectively, 2.21 ± 0.02 mm and 2.34 ± 0.08 mm in group A; 2.20 ± 0.02 mm and 2.31 ± 0.06 mm in group B; 2.20 ± 0.02 mm and 2.30 ± 0.07 mm in group C. Incision width was not significantly enlarged after phacoemulsification. Before and after IOL implantation incision width was, respectively, 2.34 ± 0.07 mm and 2.47 ± 0.07 mm in group A; 2.32 ± 0.06 mm and 2.45 ± 0.08 mm in group B; 2.30 ± 0.07 mm and 2.39 ± 0.07 mm in group C. Incision widths in group C were significantly different to groups A and B. No relationship was found between incision sizes and phacoemulsification time, ultrasound energy and IOL powers.</p></div><div><h3>Conclusion</h3><p>In cataract surgery Ultrasert™ enlarges the corneal incision less than other delivery systems.</p></div>","PeriodicalId":100782,"journal":{"name":"Journal of EuCornea","volume":"1 1","pages":"Pages 8-11"},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.xjec.2018.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75887422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

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