Chinese Journal of Eco-agriculture最新文献

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大豆光敏色素生色团合成基因GmHY2的克隆及功能分析 大豆光敏色素生色团合成基因GmHY2的克隆及功能分析
Chinese Journal of Eco-agriculture Pub Date : 2023-01-01 DOI: 10.27536/d.cnki.gccdy.2023.000007
张芷睿
{"title":"大豆光敏色素生色团合成基因GmHY2的克隆及功能分析","authors":"张芷睿","doi":"10.27536/d.cnki.gccdy.2023.000007","DOIUrl":"https://doi.org/10.27536/d.cnki.gccdy.2023.000007","url":null,"abstract":"","PeriodicalId":10032,"journal":{"name":"Chinese Journal of Eco-agriculture","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142028276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
生物炭对土壤中重金属铜、土霉素复合污染的影响研究 生物炭对土壤中重金属铜、土霉素复合污染的影响研究
Chinese Journal of Eco-agriculture Pub Date : 2023-01-01 DOI: 10.27536/d.cnki.gccdy.2023.000009
崔政武
{"title":"生物炭对土壤中重金属铜、土霉素复合污染的影响研究","authors":"崔政武","doi":"10.27536/d.cnki.gccdy.2023.000009","DOIUrl":"https://doi.org/10.27536/d.cnki.gccdy.2023.000009","url":null,"abstract":"","PeriodicalId":10032,"journal":{"name":"Chinese Journal of Eco-agriculture","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142028047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
基于压力-状态-响应模型的寒地粳稻杂交育种后代选择与实现 Selection and implementation of offspring in cold region japonica rice hybrid breeding based on pressure state response model
Chinese Journal of Eco-agriculture Pub Date : 2021-04-01 DOI: 10.13930/J.CNKI.CJEA.200776
刘宝海, Li Baohai, 聂守军, Nie Shoujun, 高世伟, Gao Shiwei, 刘晴, Liu Qing, 刘宇强, Liu Yuqiang, 常汇琳, Chang Huilin, 马成, M. Cheng, 唐铭, Tang Ming, 薛英会, Xu Yinghui, 白瑞, Bai Rui
{"title":"基于压力-状态-响应模型的寒地粳稻杂交育种后代选择与实现","authors":"刘宝海, Li Baohai, 聂守军, Nie Shoujun, 高世伟, Gao Shiwei, 刘晴, Liu Qing, 刘宇强, Liu Yuqiang, 常汇琳, Chang Huilin, 马成, M. Cheng, 唐铭, Tang Ming, 薛英会, Xu Yinghui, 白瑞, Bai Rui","doi":"10.13930/J.CNKI.CJEA.200776","DOIUrl":"https://doi.org/10.13930/J.CNKI.CJEA.200776","url":null,"abstract":"为提高育种杂交后代选择效果,引入压力-状态-响应(PSR)模型对影响寒地粳稻杂交育种后代的遗传、环境和选择因素进行探讨。构建1个目标、3个准则和18个指标组成的寒地粳稻杂交育种后代选择概念模型与评价体系,并采用客观熵权和功效评分相组合方法进行综合指数评价。结果表明:在PSR模型设计环境下,‘绥粳18’杂交育种9个世代杂交后代均表现出穗颈瘟权重值最大,其次是倒伏级别,再次是空壳率,寒地生态环境下抗穗颈瘟发病指数、抗倒伏级别和空壳率水平是水稻育种杂交后代选择最重要的考虑指标。PSR系统评价中,各子系统的影响力大小依次是响应子系统(权重为0.6867)>状态子系统(权重为0.2651)>压力子系统(权重为0.0482);各指标值变异系数为0~200.4%,大范围变异利于提高后代选择育种效果。与目前多依据株型理论选择杂交后代系谱相比,运用PSR模型理论与评价体系方法,创建动态压力选择环境,客观评价指标特征,并引入专家决策管理,能够有效克服单纯依靠育种经验、定性定量不结合、多注重性状选择以及响应决策不系统而导致多优性状聚合难、鉴定难、选择效率低等问题,具有较好可行性、可靠性和实用性,可以获得更加合理的寒地水稻育种杂交后代选择方案。本研究结果可为加快寒地优质高产多抗广适突破性水稻新品种选育提供有益参考和技术依据。","PeriodicalId":10032,"journal":{"name":"Chinese Journal of Eco-agriculture","volume":"29 1","pages":"738-750"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44170415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
垄作稻-鱼-鸡共生对水稻茎秆倒伏、穗部性状及产量的影响 垄作稻-鱼-鸡共生对水稻茎秆倒伏、穗部性状及产量的影响
Chinese Journal of Eco-agriculture Pub Date : 2021-02-01 DOI: 10.13930/J.CNKI.CJEA.200438
梁玉刚, Li Yugang, 陈奕沙, Chen Yisha, 陈璐, Chen Lu, 马微微, Mao Weiwei, 孟祥杰, Meng Xiangjie, 黄璜, Huang Huang, 余政军, YU Zheng-jun
{"title":"垄作稻-鱼-鸡共生对水稻茎秆倒伏、穗部性状及产量的影响","authors":"梁玉刚, Li Yugang, 陈奕沙, Chen Yisha, 陈璐, Chen Lu, 马微微, Mao Weiwei, 孟祥杰, Meng Xiangjie, 黄璜, Huang Huang, 余政军, YU Zheng-jun","doi":"10.13930/J.CNKI.CJEA.200438","DOIUrl":"https://doi.org/10.13930/J.CNKI.CJEA.200438","url":null,"abstract":"为了探究水稻垄作栽培和稻-鱼-鸡共生模式的结合对水稻茎秆倒伏、穗部性状及产量的影响,本文通过设计常规水稻垄作栽培(CK)、水稻垄作养鱼(RF)、水稻垄作养鸡(RC)和水稻垄作养鸡养鱼(RFC)的田间对比试验,研究垄作稻-鱼-鸡共生模式下水稻茎秆倒伏、穗部性状和实际产量的变化。结果表明:2年中4个处理的水稻株高、株鲜重、重心高度和节间长的均值整体无显著性差异。与CK处理相比,RFC和RC处理水稻茎秆节间外径、节间壁厚、穗长和穗鲜重虽呈增加趋势,但均值整体也无显著性差异;水稻产量也保持稳定。RF处理水稻茎秆节间外径、壁厚、穗长和穗鲜重均呈降低趋势,且2019年穗鲜重达显著降低(P < 0.05);水稻产量2年平均降低为29.98%(P < 0.05),其余均值整体无显著性差异。2年中RFC和RC较CK处理水稻节间茎秆抗折力平均增加19.69%和8.10%,且2年中RFC的第4和第5节间茎秆抗折力显著增加(P < 0.05);而RF处理茎秆抗弯截面模量和抗折力整体均呈降低趋势,但均值整体变化不显著。RFC和RC较CK处理水稻茎秆节间最大应力均值降低为17.85%和15.08%,倒伏指数均值降低为4.35%和4.26%,但未达显著水平;RF处理茎秆节间倒伏指数平均增加11.47%,且2018年第3和2019年第2~5节间均达显著性差异(P < 0.05)。综上所述,垄作稻-鱼-鸡共生和垄作稻-鸡共生模式能够提高水稻穗长和穗鲜重,稳定水稻产量,增加水稻茎秆节间外径和壁厚,提高茎秆抗折力和抗弯截面模量,降低茎秆最大应力和倒伏指数,从而具有一定的壮秆效应和抗倒伏能力。","PeriodicalId":10032,"journal":{"name":"Chinese Journal of Eco-agriculture","volume":"29 1","pages":"379-388"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45073827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatial variation in major water quality types and its relationships with land cover in the middle and lower reaches of Aral Sea Basin 咸海盆地中下游主要水质类型空间分异及其与土地覆被的关系
Chinese Journal of Eco-agriculture Pub Date : 2021-01-01 DOI: 10.13930/J.CNKI.CJEA.200429
Y. Zhang, X. Tan, F. Li, H. Ruan, J. Yu, Y. Gao, X. Zhai
{"title":"Spatial variation in major water quality types and its relationships with land cover in the middle and lower reaches of Aral Sea Basin","authors":"Y. Zhang, X. Tan, F. Li, H. Ruan, J. Yu, Y. Gao, X. Zhai","doi":"10.13930/J.CNKI.CJEA.200429","DOIUrl":"https://doi.org/10.13930/J.CNKI.CJEA.200429","url":null,"abstract":"Water resources and environmental issues in the Aral Sea Basin of Central Asia are global concerns. In this study, the water quality variables (i.e., basic physical and chemical attributes, different forms of nutrients, other elements, cations, and anions) from 21 sampling sites in the middle and lower reaches of Aral Sea Basin were measured in 2019 to explore water environmental variations and their causes. Spatial variation in 20 water quality variables was investigated, and the representative water quality types, spatial differences, and their causes were identified via multivariate analysis methods (i.e., principal component analysis and cluster analysis). Furthermore, the effects of land cover on the spatial variation in water quality types were explored. The results showed that: 1) the values of electronic conductivity (EC) and total dissolved solids (TDS) increased from the middle to the lower reaches, and the highest values were in the Aral Sea. This indicates that the concentrations of anions and cations increased from the middle to the lower reaches. For the nutrient variables, high phosphorous concentrations were in the middle reaches of Amu Darya, and high nitrate-nitrogen concentrations were in the Syr Darya. For the different forms of carbon, the highest concentrations were in the Amu Darya, particularly in the delta area of lower reaches. 2) The water quality at all sampling sites can be divided into three water quality types according to the similarity classification of water quality variables. The first type had low concentrations for most water quality variables, which were distributed in the middle reaches of Syr Darya and the Aral Sea. The second type had high concentrations of different forms of nitrogen and phosphorus, which were distributed in the middle and lower reaches of Amu Darya. The third type had high concentrations of carbon, anions, and cations, which were distributed in the Aral Sea. The water quality concentrations of the first and second types were mainly due to rock weathering processes on bare land, and the anions and cations were mainly derived from the weathering of silicates and evaporites. The concentrations of the third type were mainly due to the evaporation and crystallization processes of a dry climate, and the anions and cations were mainly derived from the weathering of silicates and evaporites, which may also be affected by carbonate weathering. 3) With an increase in the buffer zone radius for each sampling point (0.5 km to 10 km), the significant land cover changed from bare land to water, shrubland, grassland, mixed farmland, and vegetation for the first water quality type; the most significant land cover was water. There were no significant relationships between the second water quality type and land cover. For the third water quality type, the significant land cover changed from water to water, mixed farmland, and vegetation - the most significant land cover was water. Therefore, spatial varia","PeriodicalId":10032,"journal":{"name":"Chinese Journal of Eco-agriculture","volume":"29 1","pages":"299-311"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66581673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corn disease recognition based on the Convolutional Neural Network with a small sampling size 基于小样本量卷积神经网络的玉米病害识别
Chinese Journal of Eco-agriculture Pub Date : 2020-05-20 DOI: 10.13930/J.CNKI.CJEA.200375
Ming-tao Yang, Yao Zhang, Tao Liu
{"title":"Corn disease recognition based on the Convolutional Neural Network with a small sampling size","authors":"Ming-tao Yang, Yao Zhang, Tao Liu","doi":"10.13930/J.CNKI.CJEA.200375","DOIUrl":"https://doi.org/10.13930/J.CNKI.CJEA.200375","url":null,"abstract":"Crop disease management influences yield and quality, yet identifying corn diseases is still difficult. High labor costs, small number of sample, and uneven disease distributions contribute to the difficulty. We propose an improved Convolutional Neural Network (CNN) model based on the transfer learning method for disease identification. The sample image set was enhanced by rotation and roll-over, then the migrated MobileNetV2 model was used to train the image data set for corn diseases. The Focal Loss function was used to improve the neural network loss function, and the Softmax classification method was used for corn disease image recognition. The training set accuracy, validation set accuracy, weight, run time, and the number of parameter in six models were experimentally compared. The verification set accuracy rates were 93.88% (AlexNet), 95.48% (GoogleNet), 91.69% (Vgg16), 97.67% (RestNet34), 96.21% (MobileNetV2), and 97.23% (migrated MobileNetV2). The migrated MobileNetV2 was 97.23% accurate and weighed 8.69 MB. Confounding the MobileNetV2 model improved the recognition accuracy by 1.02% and reduced the training speed by 6 350 seconds compared to the unconfounded model. The migrated MobileNetV2 model had the best corn disease recognition ability with a small sampling size; improved convergence speed, reduced model calculations, and greatly improved the recognition time.","PeriodicalId":10032,"journal":{"name":"Chinese Journal of Eco-agriculture","volume":"28 1","pages":"1924-1931"},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42414967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
耕层土层交换对土壤氮素关键转化过程和玉米氮素利用的影响 耕层土层交换对土壤氮素关键转化过程和玉米氮素利用的影响
Chinese Journal of Eco-agriculture Pub Date : 2019-10-14 DOI: 10.13930/j.cnki.cjea.190265
杨硕 | 金文俊 | 黄海蒙 | 王军 | 周得宝 | 赵阳阳 | 董召荣 | 宋贺
{"title":"耕层土层交换对土壤氮素关键转化过程和玉米氮素利用的影响","authors":"杨硕 | 金文俊 | 黄海蒙 | 王军 | 周得宝 | 赵阳阳 | 董召荣 | 宋贺","doi":"10.13930/j.cnki.cjea.190265","DOIUrl":"https://doi.org/10.13930/j.cnki.cjea.190265","url":null,"abstract":"","PeriodicalId":10032,"journal":{"name":"Chinese Journal of Eco-agriculture","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142052372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
矿山废水灌溉区农田土壤N 2 O的产生及释放机制研究 矿山废水灌溉区农田土壤N 2 O的产生及释放机制研究
Chinese Journal of Eco-agriculture Pub Date : 2019-01-01 DOI: 10.13930/J.CNKI.CJEA.180568
常伊梅林, Chang Yimeilin, 唐常源, Tang Changyuan, 李杏, Li Xing, 李锐, Li Rui, 曹英杰, Cao Yingjie
{"title":"矿山废水灌溉区农田土壤N 2 O的产生及释放机制研究","authors":"常伊梅林, Chang Yimeilin, 唐常源, Tang Changyuan, 李杏, Li Xing, 李锐, Li Rui, 曹英杰, Cao Yingjie","doi":"10.13930/J.CNKI.CJEA.180568","DOIUrl":"https://doi.org/10.13930/J.CNKI.CJEA.180568","url":null,"abstract":"农田系统是温室气体N2O的主要排放源,目前对酸性矿山废水(acid mine drainage,AMD)灌溉影响下,农田土壤剖面N2O的来源识别、转换机制及其控制因子缺乏深入研究。本文选择广东省大宝山矿区下游沿岸水稻田和甘蔗田两种典型农田,针对酸性矿山废水灌溉区(上坝村)和天然来水灌溉区(连心村),对土壤理化性质、重金属含量及包气带N2O浓度、同位素特征值进行了测定,定量计算了硝化和反硝化作用对土壤中N2O的贡献比和N2O转化为N2的还原比,评价了其相关影响因素。结果表明:在AMD影响下,灌区农田土壤剖面N2O浓度均高于同种作物类型天然来水区土壤,同种灌溉处理下甘蔗田土壤N2O浓度高于水稻田。甘蔗田表层土壤(0~30 cm)反硝化作用对N2O产生量的贡献比高于硝化作用,约71.29% N2O由反硝化作用产生。AMD灌区甘蔗田土壤剖面中N2O还原成N2的比例随深度增加逐渐减小,在N2O浓度峰值处仅有15.54% N2O被还原成为N2,而天然来水区N2O还原成N2的平均比率高达49.80%。这表明较弱的土壤N2O还原能力导致较高浓度的N2O残留在土壤中。相关性分析表明,AMD灌溉通过改变上坝村土壤的pH、重金属含量、含水率从而改变了土壤N2O的来源途径及还原能力。组合同位素特征值溯源法有效地揭示了农田土壤N2O的来源和AMD灌区土壤的潜在生态风险,为日后的治理修复工作提供了科学依据。","PeriodicalId":10032,"journal":{"name":"Chinese Journal of Eco-agriculture","volume":"27 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66581621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
贺兰山东麓‘赤霞珠’品质形成气象条件与评级方法研究 贺兰山东麓‘赤霞珠’品质形成气象条件与评级方法研究
Chinese Journal of Eco-agriculture Pub Date : 2018-03-01 DOI: 10.13930/J.CNKI.CJEA.170579
马力文, Ma Liwen, 李剑萍, Liao Jianping, 韩颖娟, Han Yingjuan, 李万春, Li Wanchun
{"title":"贺兰山东麓‘赤霞珠’品质形成气象条件与评级方法研究","authors":"马力文, Ma Liwen, 李剑萍, Liao Jianping, 韩颖娟, Han Yingjuan, 李万春, Li Wanchun","doi":"10.13930/J.CNKI.CJEA.170579","DOIUrl":"https://doi.org/10.13930/J.CNKI.CJEA.170579","url":null,"abstract":"葡萄品质很大程度上决定了葡萄酒品质,其与气象条件关系密切。在正常管理和农业技术水平下,衡量葡萄生长气象条件是否适宜品质形成的方法称为酿酒葡萄品质气象评价技术。研究酿酒葡萄品质形成的气象评级技术和气象指标,可为葡萄酒商业评级、年份酒鉴定和窖藏提供气候参考依据。利用贺兰山东麓2003-2011年多点采样化验的101份‘赤霞珠’总糖、总酸、糖酸比、pH和单宁资料,选取其中的51份进行相关普查,确定了有生物学意义的显著气象因子,以各因子的决定系数分别建立了5项品质指标的权重模型,通过单项品质指标对综合品质的贡献构建了综合品质气象评价模型。参照前人对葡萄酒品质、酿酒葡萄品质的研究和葡萄酒和葡萄品质标准,根据酿造葡萄酒所需的总糖、总酸、糖酸比、pH和单宁,确定‘赤霞珠’各项品质指标的阈值,利用模型反推出与葡萄酒品质相对应的气象因子阈值和‘赤霞珠’品质气象分类标准。回代检验显示5项品质指标均通过了0.001的R检验和F检验,与实测值接近且变化趋势一致。总糖、总酸、糖酸比和单宁的模拟效果较好,R ≥ 0.59且RMSE较小,但pH误差相对较大。用50份未参与建模的样本估算了‘赤霞珠’品质等级。总糖、总酸与实测接近;个别样本的糖酸比、单宁误差相对较大,但变化趋势与实测一致;样本pH均在适宜范围内且较稳定。从综合评分和等级来看,28个样本与实况等级相同,18个样本误差在1级内,仅4个样本相差2级,准确反映出原料的质量。‘赤霞珠’品质气象评价指标和模型为评价贺兰山东麓酿酒葡萄品质提供了一套可行的方法。","PeriodicalId":10032,"journal":{"name":"Chinese Journal of Eco-agriculture","volume":"26 1","pages":"453-466"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48048113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
干旱条件下DCMU对高表达转C 4 - pepc 水稻的花青素合成基因及其相关信号的影响 干旱条件下DCMU对高表达转C 4 - pepc 水稻的花青素合成基因及其相关信号的影响
Chinese Journal of Eco-agriculture Pub Date : 2018-03-01 DOI: 10.13930/j.cnki.cjea.170647
何亚飞, He Yafei, 许梦洁, Xu Mengjie, 李霞, Li Xia
{"title":"干旱条件下DCMU对高表达转C 4 - pepc 水稻的花青素合成基因及其相关信号的影响","authors":"何亚飞, He Yafei, 许梦洁, Xu Mengjie, 李霞, Li Xia","doi":"10.13930/j.cnki.cjea.170647","DOIUrl":"https://doi.org/10.13930/j.cnki.cjea.170647","url":null,"abstract":"为了揭示高表达转玉米C4-磷酸烯醇式丙酮酸羧化酶(PEPC,EC 4.1.1.31)基因水稻(PC)在耐旱中光合与花青素调节途径的内在联系,本研究以PC和未转基因野生型原种(WT)的水培苗为试验材料,在4~5叶期,通过50 μmol·L-1光合抑制剂DCMU[3-(3',4'-dich-lorophenyl)-1,1-dimethyl-urea]预处理1 h,观察其在12% PEG-6000模拟干旱处理下的表现。结果表明,在模拟干旱条件下,DCMU预处理使两种供试材料相对含水量显著下降,且PC相对含量显著高于WT;干旱处理下,两种材料的花青素含量显著升高,DCMU和干旱处理使两种材料的花青素含量下调,且PC水稻中始终伴随着较高的花青素含量。光合数据表明,与单独12% PEG-6000处理相比,DCMU联合12% PEG-6000处理显著抑制了两种水稻材料的净光合速率、气孔导度、胞间CO2含量及羧化效率,但PC的各指标显著高于WT。同时,DCMU联合12% PEG-6000处理显著下调两种供试材料的内源蔗糖含量,但PC中蔗糖含量显著高于WT。进一步研究发现PC中更高的蔗糖含量与花青素合成有关转录因子bHLH(OsB1,OsB2)、R2R3-MYB(OsC1)、COP1(constitutively photomorphogenic 1)、HY5(elongated hypocotyl 5)更高的转录水平同步,下游花青素合成相关基因OsPAL、OsCHI、OsCHS、OsF3H、OsF3'H、OsDFR、OsANS的表达量增加。PC水稻可能通过诱导NO和Ca2+感受干旱信号,参与转录因子的调节,进而参与花青素合成基因的调控,合成较多的花青素,增强PC水稻对干旱逆境的响应,增强保水能力,最终表现耐旱。","PeriodicalId":10032,"journal":{"name":"Chinese Journal of Eco-agriculture","volume":"26 1","pages":"409-421"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45208576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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