Comparative Biochemistry and Physiology Part B: Comparative Biochemistry最新文献_第7页

Differential time course of liver and kidney glucose-6 phosphatase activity during fasting in rats 大鼠禁食期间肝肾葡萄糖-6磷酸酶活性的差异时程

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-09-01 DOI: 10.1016/0305-0491(94)90146-5

Carol Minassian, Gilles Mithieux

{"title":"Differential time course of liver and kidney glucose-6 phosphatase activity during fasting in rats","authors":"Carol Minassian, Gilles Mithieux","doi":"10.1016/0305-0491(94)90146-5","DOIUrl":"10.1016/0305-0491(94)90146-5","url":null,"abstract":"<div>We have studied the time course of hepatic and renal microsomal glucose-6 phosphatase (Glc-6Pase) during long-term fasting in the rat. Liver microsomal Glc-6Pase increases up to 48 hr and significantly decreases after 48 hr of fasting. The following activities were determined at 0, 24, 48, 72 and 96 hr: 0.31 ± 0.02; 0.50 ± 0.02; 0.54 ± 0.03; 0.44 ± 0.03; 0.44 ± 0.01 μmol min−1 mg protein−1, respectively (all values are means ± SEM, n = 6). Concomitantly, kidney microsomal Glc-6Pase progressively increases throughout the fast (Vm = 0.21 ± 0.01; 0.26 ± 0.004; 0.30 ± 0.01; 0.37 ± 0.02; 0.40 ± 0.01 μmolmin−1 mg protein−1, from 0 to 96 hr, respectively). These data suggest that the differential expression of Glc-6Pase activity in the liver and the kidney during long-term fasting could have an important role in the shift from a principally hepatic gluconeogenesis to a hepatic and renal gluconeogenesis.</div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 1","pages":"Pages 99-104"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90146-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18840286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Insect digestive enzymes: properties, compartmentalization and function 昆虫消化酶:性质、分类和功能

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-09-01 DOI: 10.1016/0305-0491(94)90141-4

Walter R. Terra, Clélia Ferreira

引用次数: 1034

The blood proteins of the Antarctic icefish Channichthys rhinoceratus: biological significance and purification of the two main components 南极冰鱼的血蛋白:生物学意义及两种主要成分的纯化

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-09-01 DOI: 10.1016/0305-0491(94)90145-7

G. Feller, A. Poncin, M. Aittaleb, R. Schyns, Ch. Gerday

引用次数: 19

Presence of cyclic betaines in fish 鱼体内存在环甜菜碱

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-09-01 DOI: 10.1016/0305-0491(94)90148-1

Yuji Ito, Takeshi Suzuki, Takaaki Shirai, Toshiyuki Hirano

引用次数: 7

Molecular cloning of the common carp (Cyprinus carpio) rhodopsin cDNA 鲤鱼视紫红质cDNA的分子克隆

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-09-01 DOI: 10.1016/0305-0491(94)90144-9

Huai-Jen Tsai , Su-Ru Shih , Ching-Ming Kuo , Lu-Ku Li

引用次数: 9

The primary structure of an endocuticular protein from two locust species, Locusta migratoria and Schistocerca gregaria, determined by a combination of mass spectrometry and automatic Edman degradation 用质谱法和自动Edman降解相结合的方法测定了两种蝗虫(Locusta migratoria和Schistocerca gregaria)体内蛋白质的一级结构

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-09-01 DOI: 10.1016/0305-0491(94)90149-X

Sonja Jespersen , Peter Højrup , Svend Olav Andersen , Peter Roepstorff

引用次数: 20

Some kinetic properties of amylase from the intestine of the rabbitfish, Siganus canaliculatus (Park) 兔鱼(Siganus canaliculatus)肠道淀粉酶的动力学性质

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-09-01 DOI: 10.1016/0305-0491(94)90150-3

Uma Sabapathy, L.H. Teo

引用次数: 10

Purification and characterization of a poly-l-lysine-activated serine endoprotease from Lumbricus rubellus 风疹蚓聚赖氨酸活化丝氨酸内蛋白酶的纯化及特性研究

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-09-01 DOI: 10.1016/0305-0491(94)90143-0

Kee Min Woo , Woelsung Yi , Young Jong Sohn , Chung-Soon Chang , Man-Sik Kang , Doo Bong Ha , Chin Ha Chung

引用次数: 7

Coelenterolysin: a hemolytic polypeptide associated with the coelenteric fluid of sea anemones 腔肠溶素:一种与海葵腔肠液有关的溶血多肽

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-09-01 DOI: 10.1016/0305-0491(94)90152-X

Elsa Meinardi , Julio M. Azcurra , Monica Florin-Christensen , Jorge Florin-Christensen

{"title":"Coelenterolysin: a hemolytic polypeptide associated with the coelenteric fluid of sea anemones","authors":"Elsa Meinardi , Julio M. Azcurra , Monica Florin-Christensen , Jorge Florin-Christensen","doi":"10.1016/0305-0491(94)90152-X","DOIUrl":"10.1016/0305-0491(94)90152-X","url":null,"abstract":"<div>The gastrovascular fluids of the sea anemone Phymactis clematis display strong hemolytic activity, which has basic pH optimum, is thermolabile and is sensitive to proteases. The hemolytic agent from the gastrovascular fluid was partially purified by ammonium sulfate precipitation, chromatography on Dowex-50W cation exchanger and gel filtration on Sephadex G-50. A single peak elutes from the latter with an estimated mol. wt of 18,000. This elution pattern is unaffected if the chromatography is carried out in the presence of 5 M urea. These results indicate that the hemolytic activity is due to a single peptide or a group of peptides of similar size, which we here designate as “coelenterolysin”. Coelenterolysin is also present in sea anemone tissue homogenates, is different from the nematocyst toxin and is not associated with phospholipase activities. It is inhibited by sphingomyelin. This is the first report of hemolytic polypeptides associated with the coelenteric fluid of sea anemones. Coelenterolysin may have a role in extracellular digestion, defense against predators and invasion of the coelenteron by foreign organisms.</div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 1","pages":"Pages 153-161"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90152-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74485577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

In vivo and in vitro effects of wheat germ agglutinin and Bowman-Birk soybean trypsin inhibitor, two potential transgene products, on midgut esterase and protease activities from Apis mellifera 小麦胚芽凝集素和Bowman-Birk大豆胰蛋白酶抑制剂对蜜蜂中肠酯酶和蛋白酶活性的体内外影响

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-09-01 DOI: 10.1016/0305-0491(94)90142-2

Luc P. Belzunces, Christophe Lenfant, Sylvie Di Pasquale, Marc-Edouard Colin

{"title":"In vivo and in vitro effects of wheat germ agglutinin and Bowman-Birk soybean trypsin inhibitor, two potential transgene products, on midgut esterase and protease activities from Apis mellifera","authors":"Luc P. Belzunces, Christophe Lenfant, Sylvie Di Pasquale, Marc-Edouard Colin","doi":"10.1016/0305-0491(94)90142-2","DOIUrl":"10.1016/0305-0491(94)90142-2","url":null,"abstract":"<div>Wheat germ agglutinin (WGA) and Bowman-Birk soybean trypsin inhibitor represent potential transgene products for inducing pest resistance in plants. The effects of these molecules were studied on midgut esterase and protease activities from Apis mellifera L., a major insect pollinator. Trypsin inhibitor and WGA did not exhibit an acute toxicity in A. mellifera. In vivo, trypsin inhibitor caused a decrease in the amount of trypsin activity and did not have a significant effect on esterase activity. In vitro, trypsin inhibitor inhibited about 80% of non-specific protease activity and 100% of trypsin activity. In vivo, WGA at high concentration in food (1 mg/ml) elicited a large decrease in trypsin activity and did not have a significant effect on esterase activity. In vitro, WGA did not have any significant effect on trypsin and non-specific protease activities but slightly activated esterase activity.</div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 1","pages":"Pages 63-69"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90142-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91518385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26