Comparative Biochemistry and Physiology Part B: Comparative Biochemistry最新文献_第5页

The primary structure of a basic (pI 9.0) fatty acid-binding protein from liver of Gallus domesticus 家鸡肝脏碱性(pI 9.0)脂肪酸结合蛋白的一级结构

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-10-01 DOI: 10.1016/0305-0491(94)90010-8

Fabrizio Ceciliani , Hugo L. Monaco , Severino Ronchi , Ludovica Faotto , Paola Spadon

引用次数: 38

Rates of beta-oxidation of fatty acids of various chain lengths and degrees of unsaturation in highly purified peroxisomes isolated from rat liver 从大鼠肝脏分离的高纯度过氧化物酶体中不同链长脂肪酸的β -氧化速率和不饱和程度

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-10-01 DOI: 10.1016/0305-0491(94)90011-6

David S. Chance, Michael K. McIntosh

引用次数: 6

Kinetic and regulatory properties of pyruvate kinase from Artemia embryos during incubation under aerobic and anoxic conditions. The effect of pH on the kinetic constants 在好氧和缺氧条件下，青蒿胚中丙酮酸激酶的动力学和调控特性。pH值对动力学常数的影响

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-10-01 DOI: 10.1016/0305-0491(94)90016-7

A. Lazou, A. Frosinis

{"title":"Kinetic and regulatory properties of pyruvate kinase from Artemia embryos during incubation under aerobic and anoxic conditions. The effect of pH on the kinetic constants","authors":"A. Lazou, A. Frosinis","doi":"10.1016/0305-0491(94)90016-7","DOIUrl":"10.1016/0305-0491(94)90016-7","url":null,"abstract":"<div><p>PK from <em>Artemia</em> embryos displayed sigmoidal kinetics with respect to phosphoenolpyruvate while saturation curves for ADP followed regular hyperbolas. The enzyme was strongly affected by ATP, alanine and fructose 1,6-bisphosphate, the latter increasing the affinity for PEP up to 10-fold. In order to assess whether <em>Artemia</em> PK is regulated by covalent modification, kinetic parameters were determined at various stages of development as well as after exposure of <em>Artemia</em> embryos to anoxic conditions. Incubation under anoxic conditions had no strong effect on the kinetic parameters of the enzyme. In addition, only a small effect of pH changing on the PK kinetic constants was observed. The results of the present study provide no evidence to suggest that <em>Artemia</em> PK is covalently modified in response to anoxia. Therefore, it appears that alternative mechanisms are involved in the depression of metabolic rate observed during the transition of <em>Artemia</em> embryos into anaerobic dormancy.</p></div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 2","pages":"Pages 325-332"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90016-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86798152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Glucose dehydrogenase in beef (Bos taurus) and rainbow trout (Oncorhynchus mykiss) liver: a comparative study 牛肉(Bos taurus)和虹鳟(Oncorhynchus mykiss)肝脏葡萄糖脱氢酶的比较研究

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-10-01 DOI: 10.1016/0305-0491(94)90025-6

Michael A. Tranulis, Berit Christophersen, Borgar Borrebaek

引用次数: 10

Isoenzymatic diagnosis of filariae: a method for separation of lactate dehydrogenase isoenzymes from Molinema dessetae (Nematoda: Filarioidea) 丝虫病的同工酶诊断:一种分离丝虫病中乳酸脱氢酶同工酶的方法

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-10-01 DOI: 10.1016/0305-0491(94)90028-0

Laurence Marchat , Philippe M. Loiseau , Christian Poüs , Farrethin Petek

{"title":"Isoenzymatic diagnosis of filariae: a method for separation of lactate dehydrogenase isoenzymes from Molinema dessetae (Nematoda: Filarioidea)","authors":"Laurence Marchat , Philippe M. Loiseau , Christian Poüs , Farrethin Petek","doi":"10.1016/0305-0491(94)90028-0","DOIUrl":"10.1016/0305-0491(94)90028-0","url":null,"abstract":"<div><p>Lactate dehydrogenase (LDH) is highly active in filariae and could be a valuable tool for phyllogeny studies. Unfortunately, the isoenzymatic diagnosis of filariae is often difficult for LDH because of a poor mobility of the enzymes in starch gels which are the most commonly used in such studies. We propose here a method to separate filarial LDH isoenzymes using disc electrophoresis. The experiments were carried out on male and female <em>Molinema dessetae</em> in order to compare their respective isoenzymes. The study of several parameters such as buffer systems, percentage of bisacrylamide and progression time led to optimize the enzyme separation. LDH from male and female filariae were compared to mammal LDH-H4 and LDH-M4. Five and four LDH isoenzymes were found, respectively, in male and female worms. Relative concentration of each isoenzyme diverged between male and female worms. Mammal muscle LDH-M4 type moved between LDH2 and LDH3 from female worms, and between LDH1 and LDH2 from male worms. Mammal heart H4 type enzyme was very different in electrophoretic mobility. The ratio of each isoenzyme was determined by densitometry. The major isoenzymes from female worms will be studied as a biochemical target for chemotherapeutic attack.</p></div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 2","pages":"Pages 451-457"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90028-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18560841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

The fatty acids of the sponge Hymeniacidon sanguinea from the Black Sea 黑海海绵体血膜海绵体的脂肪酸

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-10-01 DOI: 10.1016/0305-0491(94)90008-6

William W. Christie , Elizabeth Y. Brechany , Ilko N. Marekov , Kamen L. Stefanov , Stoitze N. Andreev

引用次数: 23

Nucleotides and the adenylate energy charge as indicators of stress in rainbow trout (Oncorhynchus mykiss) subjected to a range of dissolved oxygen concentrations 虹鳟鱼(Oncorhynchus mykiss)在不同溶解氧浓度下的应激指标——核苷酸和腺苷酸能量电荷

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-10-01 DOI: 10.1016/0305-0491(94)90015-9

Colleen A. Caldwell, Jeffrey M. Hinshaw

{"title":"Nucleotides and the adenylate energy charge as indicators of stress in rainbow trout (Oncorhynchus mykiss) subjected to a range of dissolved oxygen concentrations","authors":"Colleen A. Caldwell, Jeffrey M. Hinshaw","doi":"10.1016/0305-0491(94)90015-9","DOIUrl":"10.1016/0305-0491(94)90015-9","url":null,"abstract":"<div><p>Liver nucleotides (ATP, ADP, AMP, IMP), the adenylate energy charge (AEC), total adenylate concentration (TA), and IMP-load were used as measures of stress in rainbow trout (<em>Oncorhynchus mykiss</em>) acclimated to normoxic (10.0 mg/l), hypoxic (6.5 mg/l), and supersaturated (13.0 mg/l) dissolved oxygen concentrations and subjected to a challenge by confinement. Liver ATP (783.0 nmol/g) was significantly different in the normoxic fish compared to either hyperoxic (447.7 nmol/g) or hypoxic (402.0 nmol/g) fish at the end of the confinement. Within 6.0 hr in the confinement, liver AEC in the normoxic fish increased significantly (0.58) compared to hypoxic (0.42) and hyperoxic fish (0.42). Similarly, the IMP-load in normoxic fish (0.16) decreased to near prestress levels by 6.0 hr in confinement compared to either the hypoxic (0.31) or hyperoxic (0.30) fish. Nucleotides in liver were significantly affected by the dissolved oxygen treatments and the confinement stress in contrast to the muscle nucleotides which were not.</p></div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 2","pages":"Pages 313-323"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90015-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89579791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Identification of unusual cyclopropane monounsaturated fatty acids from the deep-water lake invertebrate Acanthogammarus grewingkii 深海湖底无脊椎动物棘鱼异常环丙烷单不饱和脂肪酸的鉴定

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-10-01 DOI: 10.1016/0305-0491(94)90023-X

Tomáš Řezanka , Valery M. Dembitsky

引用次数: 9

Sexual differences in blood values of the pigeon, Columba livia 鸽子血值的性别差异

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-10-01 DOI: 10.1016/0305-0491(94)90005-1

K.L. Gayathri, S.N. Hegde

引用次数: 92

Lipids of fish parasites and their hosts: fatty acids of phospholipids of Paratenuisentis ambiguus and its host eel (Anguilla anguilla) 鱼类寄生虫及其寄主的脂质:双歧鳗及其寄主鳗(Anguilla Anguilla)磷脂中的脂肪酸

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-10-01 DOI: 10.1016/0305-0491(94)90021-3

Kurt Aitzetmüller , Horst Taraschewski , Christin Filipponi , Gisela Werner , Nikolaus Weber

{"title":"Lipids of fish parasites and their hosts: fatty acids of phospholipids of Paratenuisentis ambiguus and its host eel (Anguilla anguilla)","authors":"Kurt Aitzetmüller , Horst Taraschewski , Christin Filipponi , Gisela Werner , Nikolaus Weber","doi":"10.1016/0305-0491(94)90021-3","DOIUrl":"10.1016/0305-0491(94)90021-3","url":null,"abstract":"<div><p>The fatty acid composition of various phospholipid classes, e.g. phosphatidylcholines, phosphatidylethanolamines, phosphatidylserines and sphingomyelins, of the fish endoparasite <em>Paratenuisentis ambiguus</em> and of intestinal tissue of its host, eel (<em>Anguilla anguilla</em>), were investigated. Phospholipids from parasite and infected host intestine show considerable differences in the fatty acid compositions. High proportions of saturated long-chain and very long-chain acyl moieties were found in phosphatidylcholines, ethanolamines and serines of the parasite. Membrane phospholipids of <em>P. ambiguus</em> contain remarkably high proportions of polyunsaturated acyl moieties, in particular eicosapentaenoyl moieties (20:5 n-3), which undoubtedly originate from aquatic feed or host intestine. It is suggested that the parasite's membranes are stabilized by incorporation of high proportions of saturated long-chain and very long-chain fatty acids to counterbalance the destabilizing effects of the polyunsaturated fatty acids.</p></div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 2","pages":"Pages 383-389"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90021-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88360805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7