Chang Gung medical journal最新文献_第3页

Antiviral activity of liquorice powder extract against varicella zoster virus isolated from Egyptian patients. 甘草粉提取物对埃及水痘带状疱疹病毒的抗病毒活性研究。

Chang Gung medical journal Pub Date : 2012-05-01 DOI: 10.4103/2319-4170.106149

Rania I Shebl, Magdy A Amin, Amal Emad-Eldin, Saad M Bin Dajem, Amal S Mostafa, Essam H Ibrahim, Aly F Mohamed

{"title":"Antiviral activity of liquorice powder extract against varicella zoster virus isolated from Egyptian patients.","authors":"Rania I Shebl, Magdy A Amin, Amal Emad-Eldin, Saad M Bin Dajem, Amal S Mostafa, Essam H Ibrahim, Aly F Mohamed","doi":"10.4103/2319-4170.106149","DOIUrl":"https://doi.org/10.4103/2319-4170.106149","url":null,"abstract":"Background: Varicella-zoster virus (VZV) is the etiologic agent of two diseases, varicella (chicken pox) and zoster (shingles). Varicella is a self- limited infection, while zoster is mainly a disease of adults. The present study was conducted to isolate VZV from clinically diagnosed children using cell cultures and compare the activity of liquorice powder extract, an alternative herbal antiviral agent, with acyclovir and interferon alpha 2a (IFN-α2a) against the isolated virus.Methods: Forty-eight VZV specimens, 26 from vesicular aspirates and 22 from vesicular swabs, from children clinically diagnosed with varicella were isolated on the Vero cell line. Isolates were propagated and identified with specific antiserum using indirect immunofluorescence and immunodot blotting assays. The growth kinetics of the viral isolates was studied. The antiviral activity of liquorice powder extract, acyclovir (ACV) and IFN-α2a was evaluated against the isolated virus.Results: VZV was successfully isolated in 4 of the 48 specimens, all from vesicular aspirates. The growth kinetics of the viral isolates was time dependent. The inhibitory activity of liquorice powder extract (containing 125 µg/ml glycyrrhizin) when compared to ACV (250 µg/ml) and IFN-α2a is the lowest.Conclusions: VZV isolates were successfully isolated and propagated using Vero cells. Isolates were identified using indirect immunofluorescent and immunodot blotting techniques. Growth kinetics of the isolates revealed an increase in the viral infectivity titer relative to time. Glycyrrhizin in the crude form has low antiviral activity against VZV compared with acyclovir and interferon.","PeriodicalId":10018,"journal":{"name":"Chang Gung medical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30719421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Amyloid plaque imaging from IMPY/SPECT to AV-45/PET. 淀粉样斑块成像从IMPY/SPECT到AV-45/PET。

Chang Gung medical journal Pub Date : 2012-05-01 DOI: 10.4103/2319-4170.106151

Mei-Ping Kung, Chi-Chang Weng, Kun-Ju Lin, Ing-Tsung Hsiao, Tzu-Chen Yen, Shiaw-Pyng Wey

{"title":"Amyloid plaque imaging from IMPY/SPECT to AV-45/PET.","authors":"Mei-Ping Kung, Chi-Chang Weng, Kun-Ju Lin, Ing-Tsung Hsiao, Tzu-Chen Yen, Shiaw-Pyng Wey","doi":"10.4103/2319-4170.106151","DOIUrl":"https://doi.org/10.4103/2319-4170.106151","url":null,"abstract":"The formation and deposition of β-amyloid (Aβ) plaques are the earliest pathological changes in Alzheimer's disease (AD). Molecular imaging of Aβ plaques could serve as a surrogate marker in early diagnosis and neuropathogenesis studies of AD. Several radionuclide labeled ligands have recently been developed for noninvasive visualization of Aβ plaques in the brains of AD patients using single photon emission computed tomography or positron emission tomography (PET). There has been rapid progress in the field of imaging for plaque pathology. AV-45 was the first plaque imaging agent to enter multi-center, investigational new drug clinical trials in the US, and has now been studied in dozens of trials with more than 1,000 subjects ranging from cognitively normal individuals to those with AD dementia. \"Imaging to autopsy\" phase III studies further confirmed and validated the specific imaging signal correlated to the plaque burden in living subjects. With these promising and confirmed characteristics of AV-45, the Alzheimer's Disease Neuroimaging Initiative (ADNI) under common consensus decided on AV-45 as the emerging standard PET imaging agent for evaluating the progression of plaque pathology in patients with AD or mild cognition impairment, and even healthy controls. With the wide availability of AV-45 for plaque imaging, the ultimate goal of the ADNI is global clinical trials for disease detection and progression. This review presents recent experience with Aβ-targeting radiotracers at Chang Gung University and Chang Gung Memorial Hospital.","PeriodicalId":10018,"journal":{"name":"Chang Gung medical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30719424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Optimizing aesthetic and functional outcomes at donor sites. 优化供体部位的美学和功能结果。

Chang Gung medical journal Pub Date : 2012-05-01 DOI: 10.4103/2319-4170.106150

Seng-Feng Jeng, Ngian-Chye Tan

引用次数: 10

Acute clinical deterioration of posterior fossa epidural hematoma: clinical features, risk factors and outcome. 后窝硬膜外血肿的急性临床恶化:临床特征、危险因素及预后。

Chang Gung medical journal Pub Date : 2012-05-01 DOI: 10.4103/2319-4170.106144

Tsung-Ming Su, Tsung-Han Lee, Tao-Chen Lee, Ching-Hsiao Cheng, Cheng-Hsien Lu

{"title":"Acute clinical deterioration of posterior fossa epidural hematoma: clinical features, risk factors and outcome.","authors":"Tsung-Ming Su, Tsung-Han Lee, Tao-Chen Lee, Ching-Hsiao Cheng, Cheng-Hsien Lu","doi":"10.4103/2319-4170.106144","DOIUrl":"https://doi.org/10.4103/2319-4170.106144","url":null,"abstract":"Background: This study aimed to present experience in treating patients with posterior fossa epidural hematoma (PFEDH) and to discuss the risk factors, clinical features, and outcome in PFEDH patients with acute clinical deterioration (ACD).Methods: Twenty-seven patients with PFEDH initially treated conservatively were evaluated. A comparison was made between patients with and without ACD during hospitalization.Results: Eight of the 27 patients subsequently experienced ACD. Local traumatic findings, loss of consciousness due to injury, headache, and vomiting were the four most common clinical features of the 27 cases. Seven of the 8 patients with ACD had good recoveries and 1 died. Eighteen of the 19 patients without ACD had good recoveries, while 1 remained moderately disabled. Stepwise logistic regression demonstrated that the adjusted risk of ACD during hospitalization for patients with vomiting and an occipital fracture across the transverse sinus on computed tomography (CT) had odds ratios of 12.6 (95% confidence interval = 1.03-152.37, p = 0.047) and 8.8 (95% CI = 1.02-75.95, p = 0.048), respectively, compared with those without ACD.Conclusion: This study demonstrated that an occipital fracture across the transverse sinus on CT and vomiting on presentation are risk factors for ACD during hospitalization. In this study, PFEDH patients including those with ACD had good outcomes. Further studies are necessary to clarify the clinical course and risk factors for the clinical deterioration of PFEDH patients and to establish a treatment strategy.","PeriodicalId":10018,"journal":{"name":"Chang Gung medical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30718240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Variant origins of arteries in the carotid triangle - a case report. 颈动脉三角区动脉的不同起源- 1例报告。

Chang Gung medical journal Pub Date : 2012-05-01 DOI: 10.4103/2319-4170.106143

B-V Murlimanju, Latha V Prabhu, Mangala M Pai, Dhanya Jayaprakash, Vasudha V Saralaya

引用次数: 8

Hepatoid microcarcinoma of the pancreas: a case report and review of the literature. 胰腺类肝微癌1例报告及文献复习。

Chang Gung medical journal Pub Date : 2012-05-01 DOI: 10.4103/2319-4170.106142

Shih-Chiang Huang, Hao-Cheng Chang, Ta-Sen Yeh, Kwai-Fong Ng, Tse-Ching Chen

引用次数: 16

Phosphodiesterase 4 and its inhibitors in inflammatory diseases. 磷酸二酯酶4及其抑制剂在炎性疾病中的作用。

Chang Gung medical journal Pub Date : 2012-05-01 DOI: 10.4103/2319-4170.106152

S-L Catherine Jin, Shiau-Li Ding, Shih-Chang Lin

{"title":"Phosphodiesterase 4 and its inhibitors in inflammatory diseases.","authors":"S-L Catherine Jin, Shiau-Li Ding, Shih-Chang Lin","doi":"10.4103/2319-4170.106152","DOIUrl":"https://doi.org/10.4103/2319-4170.106152","url":null,"abstract":"Type 4 cyclic nucleotide phosphodiesterases (PDE4) are a family of low km 3',5'-cyclic adenosine monophosphate (cAMP)-specific phosphodiesterases including at least 20 isozymes encoded by four genes (PDE4A, PDE4B, PDE4C, and PDE4D) in mammals. Each PDE4 gene plays a special, nonredundant role in the control of cell function even though the four subfamilies share the highly conserved catalytic domain and upstream conserved region (UCR) 1 and UCR2 motifs of the regulatory domain. By their wide tissue distribution as well as differential expression and regulation among various cell types, PDE4s are viewed as critical regulators of intracellular cAMP levels, cAMP signaling, and signal compartmentalization. By increasing cAMP levels, PDE4 inhibitors show a broad spectrum of anti-inflammatory effects in almost all inflammatory cells. Many PDE4 inhibitors have been evaluated in clinical trials for various inflammatory conditions. Developed inhibitors, including the recently approved and marketed roflumilast, have considerable efficacy, but they also have adverse effects such as nausea and emesis which limit their dosing and subsequently their immunomodulatory activity. Thus, the development of PDE4 inhibitors with improved therapeutic indexes has been a major focus of pharmaceutical research for the treatment of chronic inflammatory diseases. Recent PDE4 gene knockout studies strongly suggest that PDE4 inhibitors with PDE4B selectivity may retain the anti-inflammatory effects while limiting side effects. Development of PDE4 inhibitors with different delivery routes, such as topical application and inhalation, is also a promising approach for the treatment of pulmonary inflammatory conditions and dermatitis. This review includes a brief overview of the domain structure and function of PDE4 isozymes, the role of PDE4s in inflammatory cell responses, and the potential therapeutic utility of PDE4 inhibitors in inflammatory diseases.","PeriodicalId":10018,"journal":{"name":"Chang Gung medical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30719419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 72

Adjuvant and salvage radiotherapy for urothelial cell carcinoma of the upper urinary tract: experience in a single institution. 辅助和补救性放射治疗上尿路尿路上皮细胞癌:单一机构的经验。

Chang Gung medical journal Pub Date : 2012-05-01 DOI: 10.4103/2319-4170.106147

Kang-Hsing Fan, Yen-Chao Chen, Wei-Man Leung, Cheng-Keng Chuang, See-Tong Pang, Ji-Hong Hong

{"title":"Adjuvant and salvage radiotherapy for urothelial cell carcinoma of the upper urinary tract: experience in a single institution.","authors":"Kang-Hsing Fan, Yen-Chao Chen, Wei-Man Leung, Cheng-Keng Chuang, See-Tong Pang, Ji-Hong Hong","doi":"10.4103/2319-4170.106147","DOIUrl":"https://doi.org/10.4103/2319-4170.106147","url":null,"abstract":"Background: To investigate the role of radiotherapy (RT) for upper urinary tract urothelial cell carcinoma (UTUC) after surgery.Methods: Between July 1997 and February 2007, 40 patients who had undergone radical surgery and RT were selected. Twenty patients received RT as adjuvant treatment for advanced disease (PORT). The remainder received RT as salvage treatment (SART). The prescription dose of RT ranged from 32 to 66.6 Gy (median: 50 Gy). Cisplatin-based chemotherapy was given to 34 patients. The median follow-up was 61 months (22-93 months).Results: At the time of analysis, 10 patients were alive, but two of them had tumor recurrence. Twenty-four patients died from disease recurrence, two died from chemotherapy-related complications, and two from non-cancer comorbidities. Two patients were lost to follow-up but one of them had tumor recurrence. The 3-year overall survival (OS) was 45% for the PORT group, and 16% for the SART group (p = 0.03). The 3-year progression-free survival (PFS) was 41% for the PORT group, and 12% for the SART group (p = 0.02). A prescription dose < 50 Gy (p = 0.02) was another poor prognostic factor. The 3-year OS was 38% for a prescription dose ≥ 50 Gy, and 18% for < 50 Gy (p = 0.06). The 3-year PFS improved from 7% to 41% if the prescribed dose was ≥ 50 Gy (p < 0.05).Conclusion: According to our analysis, RT combined with chemotherapy is effective in the postoperative treatment of advanced disease and salvage treatment for recurrent UTUC. The prescription dose should be ≥ 50 Gy.","PeriodicalId":10018,"journal":{"name":"Chang Gung medical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30719423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Eccrine angiomatous hamartoma: a retrospective study of 15 cases. 15例胰腺血管瘤错构瘤的回顾性分析。

Chang Gung medical journal Pub Date : 2012-03-01 DOI: 10.4103/2319-4170.106154

Yi-Ting Lin, Chien-Ming Chen, Chih-Hsun Yang, Yea-Huey Chuang

{"title":"Eccrine angiomatous hamartoma: a retrospective study of 15 cases.","authors":"Yi-Ting Lin, Chien-Ming Chen, Chih-Hsun Yang, Yea-Huey Chuang","doi":"10.4103/2319-4170.106154","DOIUrl":"https://doi.org/10.4103/2319-4170.106154","url":null,"abstract":"Background: Eccrine angiomatous hamartoma (EAH) comprises a rare nevoid proliferation of normal eccrine glands and small blood vessels and occasionally other elements in the middle and deep dermis with variable clinical manifestations. Case series have rarely been published except for case reports and literature reviews. The aims of this article were to investigate the clinical and pathologic features of patients with EAH in Taiwan and to compare our results with the results of previous studies.Methods: A retrospective review of medical records and histopathological findings was performed on patients diagnosed with EAH in a medical center in Taiwan between 1994 and 2010.Results: Fifteen patients with pathologically diagnosed EAH were collected. The mean age at the time of diagnosis was 38.6 years (range, birth to 67 years). The male to female ratio was 3 to 2. In most cases, EAH arose as a single lesion on a lower extremity. The symptoms and signs most commonly associated with EAH were pain (60%), hypertrichosis (13.3%), itching (13.3%) and hyperhidrosis (6.7%). Additional pathological findings included hemangioma (13.3%), verrucous hemangioma (6.7%), arteriovenous malformation (6.7%), and angiokeratoma (6.7%). None of the patients experienced spontaneous regression of the lesions before excision. Excisions were done in one patient under general anesthesia, and ten patients with local anesthesia. Four patients were kept under observation. Tumor recurrences were noted in two out of the eleven patients whose lesions were excised.Conclusion: Compared with cases in the literature, we found additional histopathological findings and an increased tumor recurrence risk in our cohort. EAH remains a benign and uncommon hamartomatous condition. Further multi-center, retrospective studies with larger case numbers are needed to better characterize the disease presentation in Asian populations.","PeriodicalId":10018,"journal":{"name":"Chang Gung medical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40185736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Extract of sporoderm-broken germinating spores of ganoderma lucidum activates human polymorphonuclear neutrophils via the P38 mitogen-activated protein kinase pathway. 灵芝孢子囊破裂萌发孢子提取物通过P38丝裂原活化蛋白激酶途径激活人多形核中性粒细胞。

Chang Gung medical journal Pub Date : 2012-03-01 DOI: 10.4103/2319-4170.106158

Pei-Yu Hsu, Jiun-Liang Chern, Hsing-Yu Chen, Sien-Hung Yang

{"title":"Extract of sporoderm-broken germinating spores of ganoderma lucidum activates human polymorphonuclear neutrophils via the P38 mitogen-activated protein kinase pathway.","authors":"Pei-Yu Hsu, Jiun-Liang Chern, Hsing-Yu Chen, Sien-Hung Yang","doi":"10.4103/2319-4170.106158","DOIUrl":"https://doi.org/10.4103/2319-4170.106158","url":null,"abstract":"Background: Ganoderma lucidum (G. lucidum) has been used in traditional Chinese medicine for thousands of years because of its immunomodulatory properties. It is believed that G. lucidum enhances the human immune response by improving the function of human polymorphonuclear neutrophils (PMNs); nevertheless, the actual mechanism by which G. lucidum acts on human PMNs remains unknown. In this study, we investigated the molecular pathways through which G. lucidum activates human PMNs.Methods: The phagocytic activity of PMNs was evaluated with and without treatment with the extract of sporoderm-broken germinating spores of G. lucidum. The same activity was measured after G. lucidum treatment with or without p38 mitogen-activated protein kinase (p38 MAPK) inhibitor. The activation of p38 MAPK was also evaluated with or without treatment with the extract of sporoderm-broken germinating spores of G. lucidum.Results: In this study, we found that the extract of G. lucidum enhanced the phagocytic activity of PMNs in a dose-dependent manner, but this response was attenuated by treatment with SB203580, a p38 MAPK inhibitor. The extract of G. lucidum also enhanced activation of p38 MAPK in a dose-dependent manner.Conclusion: These results clearly show that the extract of G. lucidum can modulate human immunity by activating human PMNs via the p38 MAPK pathway. These results may be of clinical importance to doctors of traditional Chinese medicine.","PeriodicalId":10018,"journal":{"name":"Chang Gung medical journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40185800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6