Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism最新文献

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Manganese-stimulated phosphatidylinositol headgroup exchange in rat liver microsomes 锰刺激大鼠肝微粒体磷脂酰肌醇头群交换
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-08-28 DOI: 10.1016/S0005-2760(98)00083-6
Robin F. Irvine
{"title":"Manganese-stimulated phosphatidylinositol headgroup exchange in rat liver microsomes","authors":"Robin F. Irvine","doi":"10.1016/S0005-2760(98)00083-6","DOIUrl":"10.1016/S0005-2760(98)00083-6","url":null,"abstract":"<div><p>Manganese-dependent, CMP-independent incorporation of <em>myo</em>-[<sup>3</sup>H]inositol into phospholipids of rat liver microsomes was studied in an attempt to clarify the physiological significance of this headgroup-exchange reaction. The enzyme responsible worked best with Mn<sup>2+</sup> as a co-factor, but Mg<sup>2+</sup> at physiological concentrations supported a significant rate of incorporation. The <em>K</em><sub>m</sub> for <em>myo</em>-inositol was around 11 μM, yet incorporation of <em>myo</em>-[<sup>3</sup>H]inositol was unaffected by as much as 5 mM choline, ethanolamine, glycerol or serine; as this is a reversible reaction, these data imply that phosphatidylinositol is the most likely lipid substrate. Similarly, other inositols showed an apparent affinity at least two orders of magnitude lower than <em>myo</em>-inositol. Glucosamine α1–6 <em>myo</em>-inositol also had a low affinity for the enzyme, making it unlikely that this headgroup-exchange activity is part of a metabolic pathway for glycosyl phosphatidylinositols. The phosphatidylinositol radiolabelled by headgroup exchange was deacylated and deglycerated, and the resulting inositol phosphate headgroup co-chromatographed on anion exchange HPLC with <em>myo</em>-inositol 1-phosphate. The simplest interpretation of all the data is the apparent paradox that this enzyme functions at a slow rate under physiological conditions to remove the <em>myo</em>-inositol headgroup from phosphatidylinositol, only to replace it with another <em>myo</em>-inositol.</p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":"1393 2","pages":"Pages 292-298"},"PeriodicalIF":0.0,"publicationDate":"1998-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00083-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20661784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
A phospholipase A2 is transiently synthesized during seed germination and localized to lipid bodies1 磷脂酶A2在种子萌发过程中短暂合成并定位于脂质体1
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-08-28 DOI: 10.1016/S0005-2760(98)00081-2
Christian May, Regina Preisig-Müller, Michaela Höhne, Petra Gnau, Helmut Kindl
{"title":"A phospholipase A2 is transiently synthesized during seed germination and localized to lipid bodies1","authors":"Christian May,&nbsp;Regina Preisig-Müller,&nbsp;Michaela Höhne,&nbsp;Petra Gnau,&nbsp;Helmut Kindl","doi":"10.1016/S0005-2760(98)00081-2","DOIUrl":"10.1016/S0005-2760(98)00081-2","url":null,"abstract":"<div><p><span><span>A patatin-like protein is present in the storage tissue of cucumber seedlings during the stage of fat mobilization. The cucumber protein is a homologue<span> of a glycoprotein which in potatoes accounts for most of the total protein content of tubers. Following preparation of a cucumber cDNA library representing the developmental stage of cotyledons of 1 day old germinating seeds we isolated and characterized a clone encoding a patatin-like protein. Antibodies raised against the protein expressed in bacteria were used for immunodetection in subcellular fractions of cucumber seedlings. It was shown that the patatin-like protein was virtually exclusively confined to lipid bodies. The protein expressed in bacteria was characterized in vitro by its esterase activity acting on </span></span>monoacylglycerols<span> and phospholipids. Detailed analysis using various forms of phosphatidyl choline as substrates demonstrated that the patatin-like protein is a phospholipase A</span></span><sub>2</sub><span><span> acting on palmitoyl, linoleoyl and hydroperoxidized linoleoyl groups equally well. Studying the temporal and tissue-specific expression of patatin-like protein mRNA we showed its appearance exclusively during fat catabolism. As maximal amounts of the protein were found at an early stage of fat mobilization and confined to lipid bodies, we propose that the patatin-like </span>hydrolase is involved in lipid body mobilization.</span></p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":"1393 2","pages":"Pages 267-276"},"PeriodicalIF":0.0,"publicationDate":"1998-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00081-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20662398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 59
Mechanisms that account for the selective release of arachidonic acid from intact cells by secretory phospholipase A2 完整细胞通过分泌磷脂酶A2选择性释放花生四烯酸的机制
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-08-28 DOI: 10.1016/S0005-2760(98)00079-4
Alfred N. Fonteh , James M. Samet , Marc Surette , William Reed , Floyd H. Chilton
{"title":"Mechanisms that account for the selective release of arachidonic acid from intact cells by secretory phospholipase A2","authors":"Alfred N. Fonteh ,&nbsp;James M. Samet ,&nbsp;Marc Surette ,&nbsp;William Reed ,&nbsp;Floyd H. Chilton","doi":"10.1016/S0005-2760(98)00079-4","DOIUrl":"10.1016/S0005-2760(98)00079-4","url":null,"abstract":"<div><p>The current study examined mechanisms that account for the selective release of arachidonic acid (AA) from cells by secretory phospholipase A<sub>2</sub> (sPLA<sub>2</sub>). Initial studies demonstrated that low concentrations of group I and group III PLA<sub>2</sub> isotypes and an sPLA<sub>2</sub>-enriched extract from bone marrow-derived mast cells (BMMC) selectively released AA from mast cells. Much higher concentrations of group II PLA<sub>2</sub> were required to release comparable quantities of AA. Group I PLA<sub>2</sub> also selectively released AA from another mast cell line (CFTL-15) and a monocytic cell line (THP-1). In contrast, high concentrations of group I PLA<sub>2</sub> were required to release fatty acids from a promyelocytic cell line (HL-60) and this release was not selective for AA. Binding studies revealed that cell types (BMMC, CFTL-15 and THP-1) which selectively released AA also had the capacity to specifically bind group I PLA<sub>2</sub>. However, group II PLA<sub>2</sub>, which did not selectively release AA from cells, also did not specifically bind to these same cell types. Additional studies revealed that sPLA<sub>2</sub> binding to the mast cell receptor was attenuated after stimulation with antigen or ionophore A23187. Reverse transcriptase–polymerase chain reaction analyses indicated the presence of mRNA for the sPLA<sub>2</sub> receptor in BMMC, CFTL-15 and THP-1 and the absence of this mRNA in HL-60. Final studies demonstrated that <em>p</em>-aminophenyl-α-<span>d</span>-mannopyranoside BSA, a known ligand of the sPLA<sub>2</sub> receptor, also selectively released AA from mast cells but not from HL-60 cells. These experiments indicated that receptor occupancy alone (without PLA<sub>2</sub> activity) is sufficient to induce the release of AA from mast cells. Together, these data reveal that specific isotypes of sPLA<sub>2</sub> have the capacity to selectively release AA from certain cells by their capacity to bind to sPLA<sub>2</sub> receptors on the cell surface.</p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":"1393 2","pages":"Pages 253-266"},"PeriodicalIF":0.0,"publicationDate":"1998-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00079-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20662413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 44
Homocysteine stimulates the production and secretion of cholesterol in hepatic cells 同型半胱氨酸刺激肝细胞中胆固醇的产生和分泌
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-08-28 DOI: 10.1016/S0005-2760(98)00086-1
Karmin O , Edward G. Lynn , Yee H. Chung , Yaw L. Siow , Ricky Y.K. Man , P.C. Choy
{"title":"Homocysteine stimulates the production and secretion of cholesterol in hepatic cells","authors":"Karmin O ,&nbsp;Edward G. Lynn ,&nbsp;Yee H. Chung ,&nbsp;Yaw L. Siow ,&nbsp;Ricky Y.K. Man ,&nbsp;P.C. Choy","doi":"10.1016/S0005-2760(98)00086-1","DOIUrl":"10.1016/S0005-2760(98)00086-1","url":null,"abstract":"<div><p>Homocysteinemia and hypercholesterolemia are important risk factors associated with the occurrence of arteriosclerotic vascular diseases. A positive correlation between plasma levels of homocysteine and cholesterol was found in homocysteinemic patients as well as in experimental animals. In the present study, the effect of homocysteine on the production and secretion of cholesterol in human hepatoma cell line HepG2 cells was investigated. When cells were incubated with 4 mM homocysteine, the amounts of total cholesterol produced as well as the cholesterol secreted by these cells were significantly increased (from 32±5 to 74±5 nmol/mg cellular protein). Further biochemical analyses revealed that the increase in cholesterol was resulted from an enhancement in the production and secretion of the unesterified cholesterol with no concomitant change in the level of cholesteryl esters. The activity of intracellular 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was markedly elevated by 131% and 190% after cells were incubated with homocysteine for 24 and 48 h. Homocysteine also stimulated the secretion of apo B100 by HepG2 cells (from 0.84±0.11 to 1.37±0.12 μg apolipoprotein B/mg cellular protein). Our results demonstrate that homocysteine stimulates the production and secretion of cholesterol and apolipoprotein B100 in HepG2 cells. The increase in the production of cholesterol induced by homocysteine may contribute to the pathogenesis of arteriosclerosis.</p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":"1393 2","pages":"Pages 317-324"},"PeriodicalIF":0.0,"publicationDate":"1998-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00086-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20662823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 86
An evaluation of the marmoset Callithrix jacchus (sagüi) as an experimental model for the dyslipoproteinemia of human Schistosomiasis mansoni 作为人类曼氏血吸虫病异常脂蛋白血症实验模型的狐猴贾克斯狐猴(sag<e:1> i)的评价
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-08-28 DOI: 10.1016/S0005-2760(98)00076-9
Vera L.M. Lima , Vera L.M. Sena , Bruce Stewart , James S. Owen , Peter J. Dolphin
{"title":"An evaluation of the marmoset Callithrix jacchus (sagüi) as an experimental model for the dyslipoproteinemia of human Schistosomiasis mansoni","authors":"Vera L.M. Lima ,&nbsp;Vera L.M. Sena ,&nbsp;Bruce Stewart ,&nbsp;James S. Owen ,&nbsp;Peter J. Dolphin","doi":"10.1016/S0005-2760(98)00076-9","DOIUrl":"10.1016/S0005-2760(98)00076-9","url":null,"abstract":"<div><p>Human infection with the parasite <em>Schistosoma mansoni</em> is a relatively common occurrence in regions of South America and is associated with liver dysfunction and dyslipoproteinemia. Specifically, the activity of plasma lecithin:cholesterol acyltransferase (LCAT) activity is reduced, the concentration of plasma cholesterol esters falls, phospholipid concentrations are elevated and erythrocyte membranes become cholesterol enriched. Previous studies have utilized rodents (rats and mice) as experimental models to study the dyslipoproteinemia induced by <em>S. mansoni</em> infection. However, the plasma lipoprotein profiles in these animals is very different from humans and infection is not accompanied by decreases in LCAT activity or cholesterol enrichment of their erythrocyte membranes. Here we have evaluated the suitability of the marmoset <em>Callithrix jacchus</em> (sagüi) which is small and readily available in Brazil, as a potential animal model for the study of the dyslipoproteinemia of <em>S. mansoni</em> infections. The plasma lipoprotein compositions and distributions in sagüi, unlike rats or mice, approximate those of man with the LDL representing a major lipoprotein species. The molecular species of phospholipids, cholesterol esters and triglycerides present in sagüi plasma are also very similar to man, whereas those of rats and mice favor the longer chain more unsaturated species. Sagüi, like rodents, can be successfully infected with <em>S. mansoni</em> and after 60 days, this results in a 50% reduction in plasma LCAT activity, an 11% reduction in plasma cholesterol esters, an absolute increase of 46% in plasma phospholipids and an 18% increase in the cholesterol content of erythrocyte membranes. These changes are qualitatively and quantitatively very similar to those previously reported following human infections. Based upon these changes, and the observation that the plasma lipoprotein profile of sagüi and human is similar, we conclude that <em>C. jacchus</em> (sagüi) is an appropriate animal model for the study of dyslipoproteinemia associated with <em>S. mansoni</em> infections.</p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":"1393 2","pages":"Pages 235-243"},"PeriodicalIF":0.0,"publicationDate":"1998-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00076-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20663851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Enhancement of phospholipase D activity following baculovirus and adenovirus infection in Sf9 and COS-7 cells 杆状病毒和腺病毒感染Sf9和COS-7细胞后磷脂酶D活性的增强
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-08-28 DOI: 10.1016/S0005-2760(98)00087-3
Ariane Höer, Torsten Schöneberg, Christian Harteneck, Cigdem Cetindag, Eckard Oberdisse
{"title":"Enhancement of phospholipase D activity following baculovirus and adenovirus infection in Sf9 and COS-7 cells","authors":"Ariane Höer,&nbsp;Torsten Schöneberg,&nbsp;Christian Harteneck,&nbsp;Cigdem Cetindag,&nbsp;Eckard Oberdisse","doi":"10.1016/S0005-2760(98)00087-3","DOIUrl":"10.1016/S0005-2760(98)00087-3","url":null,"abstract":"<div><p>In order to purify the human phospholipase D1 (hPLD1) for analysis of its functional properties, we applied a baculovirus-based high-expression system. As expected, Sf9 cells infected with a baculovirus encoding for the hPLD1 displayed a 7.5-fold increase in PLD activity compared to uninfected cells. Sf9 cells infected with the wild-type (WT) and other recombinant baculoviruses were used as an expression control. Surprisingly, all baculoviruses tested led to a 3–5-fold increase in basal PLD activity when compared to uninfected cells. To further characterize the nature of the increased PLD activity, the influence of ADP-ribosylation factor (ARF) and phorbol 12-myristate 13-acetate (PMA) was studied. In contrast to membranes containing the hPLD1, the PLD activity in membranes from uninfected and WT-infected Sf9 cells was not stimulated by ARF. PMA did not affect the increase in PLD activity in any case. To further study whether the virus-mediated increase in PLD activity is a more general phenomenon, we infected COS-7 cells with recombinant and WT adenoviruses. Only the infection with the WT adenovirus resulted in an approx. 2-fold increase in PLD activity. Our results demonstrate for the first time that a viral infection elevates the PLD activity in insect and mammalian cells.</p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":"1393 2","pages":"Pages 325-335"},"PeriodicalIF":0.0,"publicationDate":"1998-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00087-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20662189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Catabolic fate of dietary trilinoleoylglycerol hydroperoxides in rat gastrointestines 膳食中三叶油基甘油氢过氧化物在大鼠胃肠道中的分解代谢命运
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-08-28 DOI: 10.1016/S0005-2760(98)00088-5
Kazuki Kanazawa, Hitoshi Ashida
{"title":"Catabolic fate of dietary trilinoleoylglycerol hydroperoxides in rat gastrointestines","authors":"Kazuki Kanazawa,&nbsp;Hitoshi Ashida","doi":"10.1016/S0005-2760(98)00088-5","DOIUrl":"10.1016/S0005-2760(98)00088-5","url":null,"abstract":"<div><p>To elucidate whether dietary lipid peroxides are absorbed in the body, the catabolic fate of trilinoleoylglycerol hydroperoxides (TL-OOH), in the gastrointestines of rats was examined. Oxidized trilinoleoylglycerol with a peroxide value of 1000 meq/kg, 0.5 or 20 mg, was dosed intragastrically to rat together with 59.5 or 40 mg unoxidized trilinoleoylglycerol, respectively. The fate of TL-OOH in gastric and intestinal lumina was determined by high-performance liquid chromatography periodically until 240 min after treatment. At low dose, TL-OOH was soon broken down to linoleic acid hydroperoxides (LA-OOH) and hydroxyls, probably through gastric lipases, whereas at high dose, TL-OOH was retained in the stomach. In both cases, TL-OOH did not reach the intestines, though the unoxidized lipids moved to the intestines. When LA-OOH was given intragastrically, the lipids decomposed in the stomach, and linoleic acid hydroxyls, hexanal, 9-oxononanoic acid, and two novel compounds were detected 30 min after treatment. The novel compounds were identified to be epoxyketones, 11-oxo-12,13-epoxy-9- and 11-oxo-9,10-epoxy-12-octadecenoic acids. Thus, dietary TL-OOH was broken down in the stomach releasing LA-OOH which decomposed further, and did not reach the intestines.</p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":"1393 2","pages":"Pages 336-348"},"PeriodicalIF":0.0,"publicationDate":"1998-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00088-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20662191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 47
The role of dietary choline in the beneficial effects of lecithin on the secretion of biliary lipids in rats 膳食胆碱在卵磷脂对大鼠胆脂分泌的有益作用中的作用
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-08-28 DOI: 10.1016/S0005-2760(98)00072-1
Marie-Josée LeBlanc , Victor Gavino , Aline Pérea , Ibrahim M Yousef , Emile Lévy , Beatriz Tuchweber
{"title":"The role of dietary choline in the beneficial effects of lecithin on the secretion of biliary lipids in rats","authors":"Marie-Josée LeBlanc ,&nbsp;Victor Gavino ,&nbsp;Aline Pérea ,&nbsp;Ibrahim M Yousef ,&nbsp;Emile Lévy ,&nbsp;Beatriz Tuchweber","doi":"10.1016/S0005-2760(98)00072-1","DOIUrl":"10.1016/S0005-2760(98)00072-1","url":null,"abstract":"<div><p>Earlier studies showed that dietary soybean lecithin increases biliary lipid secretion, which mainly comes from the contribution of high density lipoprotein (HDL) and hepatic microsomal pools of phosphatidylcholine and cholesterol. In addition, a lecithin diet enhances bile secretion and prevents bile acid-induced cholestasis. This study evaluated the contribution of choline, a component of lecithin, to the observed effect of lecithin on biliary secretory function. Rats were fed either a control diet (CD), a choline diet (ChD) or a lecithin-enriched diet (LD) for 2 weeks. Results showed that like LD, ChD induced an increase in bile flow and bile acid secretion rate when compared with the control diet. However, unlike LD, ChD did not significantly increase biliary phospholipids and cholesterol output. An increase of hydrophilic bile acids (i.e. ursodeoxycholic and muricholic acids) in bile of rats fed choline could explain why the biliary phospholipid and cholesterol secretion was not increased. During taurocholic acid infusion, both experimental diets increased bile flow and the bile acid secretion rate maximum (BASRm). The cholestasis usually observed after the BASRm is reached was inhibited by ChD and LD. Both diets induced a decrease in plasma cholesterol (total and HDL), however, only LD induced statistically significant changes. Analysis of total cholesterol and phospholipid content of microsomes and canalicular membranes indicated no statistically significant difference between control and experimental groups, either under basal conditions or after bile acid infusion. Similarly, the phospholipid classes and fatty acid composition of biliary phosphatidylcholine were not altered by feeding ChD and LD. We conclude that choline contributes to the beneficial effect of a lecithin diet on bile secretion. It is postulated that this effect may be attributed to modulation of HDL and an enhancement of the cholesterol and phospholipid pools destined for biliary secretion.</p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":"1393 2","pages":"Pages 223-234"},"PeriodicalIF":0.0,"publicationDate":"1998-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00072-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20662656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 34
An intracellular role for pancreatic bile salt-dependent lipase: evidence for modification of lipid turnover in transfected CHO cells 胰腺胆盐依赖性脂肪酶的细胞内作用:在转染CHO细胞中脂质转换的证据
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-08-28 DOI: 10.1016/S0005-2760(98)00085-X
Josette Le Petit-Thevenin, Nadine Bruneau, Odette Nobili, Dominique Lombardo, Alain Vérine
{"title":"An intracellular role for pancreatic bile salt-dependent lipase: evidence for modification of lipid turnover in transfected CHO cells","authors":"Josette Le Petit-Thevenin,&nbsp;Nadine Bruneau,&nbsp;Odette Nobili,&nbsp;Dominique Lombardo,&nbsp;Alain Vérine","doi":"10.1016/S0005-2760(98)00085-X","DOIUrl":"10.1016/S0005-2760(98)00085-X","url":null,"abstract":"<div><p>Pancreatic bile salt-dependent lipase (BSDL) hydrolyzes cholesteryl esters, triglycerides and phospholipids. BSDL is also capable of transferring free fatty acid to cholesterol. BSDL has been detected in many cells including fetal and tumor cells, hepatocytes, macrophages and eosinophils and in tissues such as adrenal glands and testes. The enzyme may be secreted or located within subcellular compartments such as the endoplasmic reticulum or the cytosol. Although the role of the secreted enzyme is well documented, that of the intracellular form(s) is still hypothetical. In the present study, we addressed the effects of BSDL on cell lipid metabolism. For that purpose, the cDNA of rat BSDL was transfected into CHO K1 cells (CHO K1-BSDL clone) which were then loaded with [<sup>3</sup>H]oleic acid. The results demonstrate that the transfected BSDL is secreted; in spite of that, a large fraction of catalytically active BSDL is found in cell lysate. The lipid metabolism of transfected cells is affected and BSDL induces an enhanced incorporation of [<sup>3</sup>H]oleic acid in cholesteryl esters whereas fatty acid incorporation in phosphatidylcholine is decreased. These effects were particularly important in the cytosol of transfected cells where transfected BSDL preferentially locates. These data suggested that BSDL could be implicated in the cycle of the cellular homeostasis of cholesterol which is particularly affected in tumoral cells leading to cholesteryl ester storage within cytosolic lipid droplets.</p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":"1393 2","pages":"Pages 307-316"},"PeriodicalIF":0.0,"publicationDate":"1998-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00085-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20662821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Methylene-interrupted double bond in polyunsaturated fatty acid is an essential structure for metabolism by the fatty acid chain elongation system of rat liver 多不饱和脂肪酸中的亚甲基中断双键是大鼠肝脏脂肪酸链延伸系统代谢所必需的结构
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-08-28 DOI: 10.1016/S0005-2760(98)00084-8
Tamotsu Tanaka, Toshinori Hattori, Maki Kouchi, Kaoru Hirano, Kiyoshi Satouchi
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引用次数: 30
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