中国生物化学与分子生物学报Pub Date : 2015-12-20Epub Date: 2015-12-24DOI: 10.13865/j.cnki.cjbmb.2015.12.06
Nil Vanli, H U Guo-Fu
{"title":"Mechanism and Function of Angiogenin in Prostate Cancer.","authors":"Nil Vanli, H U Guo-Fu","doi":"10.13865/j.cnki.cjbmb.2015.12.06","DOIUrl":"https://doi.org/10.13865/j.cnki.cjbmb.2015.12.06","url":null,"abstract":"<p><p>Angiogenin (ANG), the fifth member of the vertebrate-specific ribonuclease (RNase) A superfamily, is a secreted angiogenic ribonuclease strongly up-regulated in human prostate cancers. ANG is translocated to the nucleus in both prostate cancer epithelial cells and endothelial cells to exert its role in prostate cancer progression by mediating tumor angiogenesis, cancer cell survival and proliferation through rRNA biogenesis. ANG-stimulated rRNA is required not only for prostate intraepithelial neoplasia (PIN) formation, but also for androgen-independent growth of prostate cancer cells. Targeting ANG by various antagonists that inhibit its nuclear translocation, function and/or activity has proven to inhibit prostate cancer growth in animal models. Furthermore, the role of ANG in androgen independence has been firmly established, suggesting a strong rationale for therapeutically targeting ANG in the treatment of castration resistant prostate cancer.</p>","PeriodicalId":9907,"journal":{"name":"中国生物化学与分子生物学报","volume":"31 12","pages":"1261-1266"},"PeriodicalIF":0.0,"publicationDate":"2015-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4862603/pdf/nihms756046.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34544493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中国生物化学与分子生物学报Pub Date : 2015-12-01DOI: 10.13865/J.CNKI.CJBMB.2015.12.05
L. Shu-ping, H. Guo-fu
{"title":"Mechanism and Function of Angiogenin in Apoptosis Regulation.","authors":"L. Shu-ping, H. Guo-fu","doi":"10.13865/J.CNKI.CJBMB.2015.12.05","DOIUrl":"https://doi.org/10.13865/J.CNKI.CJBMB.2015.12.05","url":null,"abstract":"Angiogenin (ANG), a secreted ribonuclease, has been characterized recently as an anti-apoptosis factor involved in a variety of cellular anti-apoptosis process. ANG regulates intrinsic pathways-related major molecules such as anti-apoptotic protein Bcl-2, as well as extrinsic signaling pathways. Moreover, ANG regulates p53-regulated apoptosis, a process considered to be important in regulating both the extrinsic and the intrinsic pathways.","PeriodicalId":9907,"journal":{"name":"中国生物化学与分子生物学报","volume":"31 12 1","pages":"1258-1260"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66559352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中国生物化学与分子生物学报Pub Date : 2011-11-28DOI: 10.3760/CMA.J.ISSN.1006-9801.2011.11.007
Xiu-hong Jia, W. Fan, Jian-chang Li
{"title":"Screening and Verification of the siRNA Targeted for the FBXL20 Gene","authors":"Xiu-hong Jia, W. Fan, Jian-chang Li","doi":"10.3760/CMA.J.ISSN.1006-9801.2011.11.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1006-9801.2011.11.007","url":null,"abstract":"Objective To obtain effective siRNA fragment of HOXA10 gene and verify its function,to supply experimental evidence for tumor prevention and curation by RNAi targeting to HOXA10 gene.Methods Three pairs of small interfering RNA targeting to the different sites of HOXA10 were designed and introduced into A549.The mRNA expression of HOXA10 of A549 was detected by semi-quantitative RT-PCR,the cell proliferation was assayed by MTT,the apoptosis was measured by flow cytometry.The most effective siRNA assay was screened and was tested the relationship between it and proliferation and apoptosis.Results The mRNA of HOXA10 was inhibited by three siRNAs in A549 cells,among which siRNA1 gave the strongest inhibiting of HOXA10 by ODR was (20.190±1.698) ％.The inhibitory rate of cell proliferation was (69.793±2.092) ％ and the apoptosis rate was (29.593±2.670) ％.Conclusion siRNA1 can specifically degrade HOXA10 mRNA and inhibit the proliferation of A549 cell and promote its apoptosis. \u0000 \u0000Key words: \u0000Neoplasms; Genes, homeobox; RNA interference; A549 cell","PeriodicalId":9907,"journal":{"name":"中国生物化学与分子生物学报","volume":"37 1","pages":"743-746"},"PeriodicalIF":0.0,"publicationDate":"2011-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85359410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
求助内容：
应助结果提醒方式：