Cell Stress and Chaperones最新文献

Identification and functional characterization of a novel TRPA1 gene from sea cucumber Apostichopus japonicus and interaction with miR-2013 in response to salt stress 海参 Apostichopus japonicus 的新型 TRPA1 基因的鉴定和功能表征以及在盐胁迫反应中与 miR-2013 的相互作用

Cell Stress and Chaperones Pub Date : 2023-12-18 DOI: 10.1007/s12192-023-01398-x

Xin Wei, Haoran Pan, Dan Liu, Xinyan Zhao, Yuqing Gou, Ran Guo, Yi Tian

{"title":"Identification and functional characterization of a novel TRPA1 gene from sea cucumber Apostichopus japonicus and interaction with miR-2013 in response to salt stress","authors":"Xin Wei, Haoran Pan, Dan Liu, Xinyan Zhao, Yuqing Gou, Ran Guo, Yi Tian","doi":"10.1007/s12192-023-01398-x","DOIUrl":"https://doi.org/10.1007/s12192-023-01398-x","url":null,"abstract":"Salinity is important abiotic factor influencing sea cucumber aquaculture. This study aimed to identify and functional study of a novel transient receptor potential cation channel subfamily A member 1 (TRPA1) involved in salinity stress through interaction with miR-2013 in the sea cucumber. The full-length cDNA sequence was 1369 bp in length and encoded 138 amino acids. The TRPA1 homolog protein was a hydrophilic protein without a signal peptide and was predicted to a spatial structure of seven helices and eight random coils and two major ANK functional domains. Bioinformatic analysis and luciferase reporter assays confirmed TRPA1 as a target gene of miR-2013. Quantitative PCR revealed that miR-2013 was induced upregulation after salinity stress, while TRPA1 showed upregulated expression with maximum expression at 24 h. The expression of miR-2013 and TRPA1 was negatively regulated. Transfection experiments were conducted to validate the role of miR-2013 and TRPA1 in salinity response. The results showed that miR-2013 was upregulated and TRPA1 was downregulated after transfection with miR-2013 mimics, while miR-2013 was downregulated and TRPA1 was upregulated after transfection with miR-2013 inhibitor. Transfection with si-TRPA1 homolog resulted in upregulation of miR-2013 and downregulation of TRPA1 homolog. These findings suggest that miR-2013 can regulate the expression of TRPA1 under salt stress, and highlight the importance of miR-2013 and TRPA1 in salt stress response. miR-2013 mimics improved the survival rate, while miR-2013 inhibitor and si-TRPA1 reduced it. These findings suggest that miR-2013 and TRPA1 play important roles in sea cucumbers adaptation to salinity changes.","PeriodicalId":9812,"journal":{"name":"Cell Stress and Chaperones","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138715552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In memoriam: Ian R. Brown (1943–2020) 纪念:伊恩·r·布朗(1943-2020)

Cell Stress and Chaperones Pub Date : 2022-07-01 DOI: 10.1007/s12192-022-01289-7

R. Currie, L. Hightower

引用次数: 0

Heme as a target for protection against doxorubicin-induced apoptosis in H9c2 cardiomyocytes. 血红素是防止 H9c2 心肌细胞在多柔比星诱导下凋亡的靶标。

Cell Stress and Chaperones Pub Date : 2019-11-01 Epub Date: 2019-11-18 DOI: 10.1007/s12192-019-01045-4

Na Liu, Liangqiang Zou, Mei Hu, Man Zhang

{"title":"Heme as a target for protection against doxorubicin-induced apoptosis in H9c2 cardiomyocytes.","authors":"Na Liu, Liangqiang Zou, Mei Hu, Man Zhang","doi":"10.1007/s12192-019-01045-4","DOIUrl":"10.1007/s12192-019-01045-4","url":null,"abstract":"Heme homeostasis is of vital importance to many biological processes associated with cell redox activity. However, the role of heme in the doxorubicin (DOX)-induced cardiotoxicity is still not clear. The aim of the present study was to test the hypothesis that heme is related to the DOX-induced oxidative stress and inhibition of heme expression may protect H9c2 cardiomyocytes against DOX-induced cardiotoxicity. For the evaluation of heme changing under doxorubicin treatment, H9c2 cells were treated with 0.5, 1, 2, and 4 mg/mL doxorubicin respectively. H9c2 cells were divided into 5 groups: Control group (cells were cultured without intervention), DOX group (cells were treated with 2 mg/mL doxorubicin for 6 h), Heme depletion+DOX group (cells were cultured with heme-depleted serum media, 0.5 mM succinylacetone and 2 mg/mL doxorubicin), Heme group (cells were treated with 30 μM heme), and Heme depletion+DOX+Heme group. Apoptotic cells were detected by flow cytometry with Annexin V-FITC/PI. The intracellular oxidant levels were measured by DCFH-DA fluorescence. The levels of heme were detected by ELISA. Doxorubicin significantly increased intracellular heme level from 5013 ± 187 ng/mL to the highest level of 11,720 ± 107 ng/mL, as well as the intracellular oxidants and cell apoptosis rate elevated by the increase of doxorubicin concentration. Heme depletion can significantly suppress the DOX-induced apoptosis from 39.8 ± 0.5% to 20.8 ± 0.5% (p < 0.001). Re-supplemented with exogenous heme partially but significantly restored the DOX-induced apoptosis. Heme plays an important role in doxorubicin toxicity-induced cardiomyocyte injury. By appropriate reduction in the accumulation of free heme in cardiomyocytes, doxorubicin-induced cardiotoxicity may be alleviated.","PeriodicalId":9812,"journal":{"name":"Cell Stress and Chaperones","volume":"24 1","pages":"1211-1217"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85368667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative phosphoproteome and proteome analyses emphasize the influence of phosphorylation events during the nutritional stress of Trypanosoma cruzi: the initial moments of in vitro metacyclogenesis. 定量磷酸化蛋白质组和蛋白质组分析强调了在克氏锥虫营养应激过程中磷酸化事件的影响：体外新陈代谢的初始时刻。

Cell Stress and Chaperones Pub Date : 2019-09-01 Epub Date: 2019-07-31 DOI: 10.1007/s12192-019-01018-7

Aline Castro Rodrigues Lucena, Juliana Carolina Amorim, Carla Vanessa de Paula Lima, Michel Batista, Marco Aurelio Krieger, Lyris Martins Franco de Godoy, Fabricio Klerynton Marchini

{"title":"Quantitative phosphoproteome and proteome analyses emphasize the influence of phosphorylation events during the nutritional stress of Trypanosoma cruzi: the initial moments of in vitro metacyclogenesis.","authors":"Aline Castro Rodrigues Lucena, Juliana Carolina Amorim, Carla Vanessa de Paula Lima, Michel Batista, Marco Aurelio Krieger, Lyris Martins Franco de Godoy, Fabricio Klerynton Marchini","doi":"10.1007/s12192-019-01018-7","DOIUrl":"10.1007/s12192-019-01018-7","url":null,"abstract":"Phosphorylation is an important event in cell signaling that is modulated by kinases and phosphatases. In Trypanosoma cruzi, the etiological agent of Chagas disease, approximately 2% of the protein-coding genes encode for protein kinases. This parasite has a heteroxenic life cycle with four different development stages. In the midgut of invertebrate vector, epimastigotes differentiate into metacyclic trypomastigotes in a process known as metacyclogenesis. This process can be reproduced in vitro by submitting parasites to nutritional stress (NS). Aiming to contribute to the elucidation of mechanisms that trigger metacyclogenesis, we applied super-SILAC (super-stable isotope labeling by amino acids in cell culture) and LC-MS/MS to analyze different points during NS. This analysis resulted in the identification of 4205 protein groups and 3643 phosphopeptides with the location of 4846 phosphorylation sites. Several phosphosites were considered modulated along NS and are present in proteins associated with various functions, such as fatty acid synthesis and the regulation of protein expression, reinforcing the importance of phosphorylation and signaling events to the parasite. These modulated sites may be triggers of metacyclogenesis.","PeriodicalId":9812,"journal":{"name":"Cell Stress and Chaperones","volume":"24 1","pages":"927-936"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81458339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The reality of scientific research in Latin America; an insider’s perspective 拉丁美洲科学研究的现实;局内人的视角

Cell Stress and Chaperones Pub Date : 2017-01-01 DOI: 10.1007/s12192-017-0814-9

D. Ciocca, G. Delgado

引用次数: 36

In memoriam Wilfried de Jong (1942 – 2016)

Cell Stress and Chaperones Pub Date : 2016-10-18 DOI: 10.1007/s12192-016-0738-9

Peter Bloemers, W. Boelens

引用次数: 0

Erratum to: A novel small molecule HSP90 inhibitor, NXD30001, differentially induces heat shock proteins in nervous tissue in culture and in vivo 一种新型小分子HSP90抑制剂NXD30001在培养和体内神经组织中诱导热休克蛋白的差异

Cell Stress and Chaperones Pub Date : 2013-11-19 DOI: 10.1007/s12192-013-0476-1

Jieun Cha, Kyle J. H. St. Louis, Benoit J. Gentil, Michael Tibshirani, Miranda L. Tradewell, S. Minotti, Z. Jaffer, Ruihong Chen, A. Rubenstein, H. Durham

引用次数: 1

Erratum to: Acute phase proteins are major clients for the chaperone action of α2-macroglobulin in human plasma 急性期蛋白是人血浆中α - 2巨球蛋白伴侣作用的主要对象

Cell Stress and Chaperones Pub Date : 2013-07-05 DOI: 10.1007/s12192-013-0441-z

A. Wyatt, N. Zammit, Mark R. Wilson

引用次数: 1

Erratum to: Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock 热休克期间HSF1和HSF4对α b -结晶蛋白启动子的细胞类型依赖性通路的更正

Cell Stress and Chaperones Pub Date : 2013-03-28 DOI: 10.1007/s12192-013-0419-x

Zhe Jing, Rajendra K. Gangalum, J. Lee, D. Mock, S. Bhat

引用次数: 1

Erratum to: Dynamics of endogenous Hsp70 synthesis in the brain of olfactory bulbectomized mice 嗅球切除小鼠脑内源性热休克蛋白70合成动力学的勘误

Cell Stress and Chaperones Pub Date : 2012-11-06 DOI: 10.1007/s12192-012-0381-z

N. Bobkova, I. Guzhova, B. Margulis, I. Nesterova, N. Medvinskaya, A. Samokhin, I. Alexandrova, D. Garbuz, E. Nudler, M. Evgen’ev

引用次数: 3