Harmful algae最新文献

A brown seaweed Sargassum fusiforme polysaccharides protect against ischemic stroke by mediating angiogenic factors through the modulation of the HIF-1α/VEGF pathway 褐藻马尾藻多糖通过调节HIF-1α/VEGF通路介导血管生成因子，对缺血性卒中具有保护作用

Harmful algae Pub Date : 2025-12-15 DOI: 10.4490/algae.2025.40.11.27

Likun Chen, Xingyue He, YuXin Bai, Meijun Liu, Ying Meng, Mingdian Wu, Peng Zheng, Yulin Dai

{"title":"A brown seaweed Sargassum fusiforme polysaccharides protect against ischemic stroke by mediating angiogenic factors through the modulation of the HIF-1α/VEGF pathway","authors":"Likun Chen, Xingyue He, YuXin Bai, Meijun Liu, Ying Meng, Mingdian Wu, Peng Zheng, Yulin Dai","doi":"10.4490/algae.2025.40.11.27","DOIUrl":"https://doi.org/10.4490/algae.2025.40.11.27","url":null,"abstract":"Ischemic stroke (IS) has emerged as one of the leading causes of mortality and disability in adults worldwide. Hypoxia-inducible factor 1α (HIF-1α) signaling pathway plays a critical role in endogenous neuroprotective mechanisms following IS. Sargassum fusiforme is a significant marine algal resource. In previous research, we isolated bioactive fucoidan from acid-processed S. fusiforme, and demonstrated its anti-inflammatory and antioxidant efficacy. This study aimed to elucidate the role of S. fusiforme polysaccharides (SFPS) in promoting angiogenesis via the HIF 1α/vascular endothelial growth factor (VEGF) pathway following IS and to investigate the underlying mechanisms involved. In this study, we employed a hypoxia model in human cerebral microvascular endothelial cells (hCMECs/ D3) and a zebrafish IS model to elucidate the therapeutic mechanisms of SFPS in mitigating cerebral ischemic injury. Cellular assays confirmed the ability of fucoidan to prevent apoptosis and promote neovascularization in hCMEC/D3 cells under hypoxic stress. Furthermore, in a zebrafish IS model, fucoidan increased blood flow velocity and locomotor activity, while reducing neuronal apoptosis. Mechanistically, fucoidan activated the HIF-1α/VEGF signaling pathway in both cellular and animal models, thereby ameliorating hypoxic injury and stimulating angiogenesis. Results demonstrated the angiogenic activity of fucoidan in both in vitro and in vivo models, and revealed its key targets and associated pathways underlying its anti-ischemic effects. Findings collectively highlight the prospective utility of fucoidan as a marine-derived therapeutic agent from traditional Chinese medicine for neurovascular repair.","PeriodicalId":94320,"journal":{"name":"Harmful algae","volume":"40 4","pages":"371-386"},"PeriodicalIF":0.0,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental evidence for the selection of salinity-tolerant ecotypes of the Microcystis aeruginosa complex. 铜绿微囊藻复合体耐盐生态型选择的实验证据。

IF 4.5

Harmful algae Pub Date : 2025-11-01 Epub Date: 2025-08-07 DOI: 10.1016/j.hal.2025.102936

Gabriela Martínez de la Escalera, Angel M Segura, Carla Kruk, Carolina González, Claudia Piccini

{"title":"Experimental evidence for the selection of salinity-tolerant ecotypes of the Microcystis aeruginosa complex.","authors":"Gabriela Martínez de la Escalera, Angel M Segura, Carla Kruk, Carolina González, Claudia Piccini","doi":"10.1016/j.hal.2025.102936","DOIUrl":"10.1016/j.hal.2025.102936","url":null,"abstract":"Microcystis aeruginosa complex (MAC) generate harmful blooms in multiple aquatic ecosystems, from freshwater to estuaries. The intra-complex genetic diversity and its phenotypic plasticity emerge as the main hypothesis of ecological success of the group in eutrophic ecosystems. This intra-complex diversity is composed of ecotypes, phylogenetically closely related organisms differing in their ecological preferences that could be considered species. Under the hypothesis of a rapid selection of ecotypes caused by salinity, here, we experimentally evaluated the role of salinity (0, 5, 10 and 25 ppt) in the ecotype community configuration and toxicity of MAC. We assessed the response to different salinities by analyzing ecotype composition (mcyJ based genotyping) combined with mcyJ amplicon sequencing for ecotypes identification, MC-producing cells abundance and their toxin-production activity (mcy gene copies and transcripts abundance, and MC variants concentration), and machine learning methods for data analysis. We found that, although the abundance of MC-producing cells, the mcyE transcription and MC concentration were negatively affected by increasing salinity, the treatments at 10 and 25 ppt selected MAC ecotypes showing different profiles of MC variants. The results indicate that when freshwater MAC species are transported to brackish waters, the salt-induced stress (salinities between 5 and 10 ppt) selects salt-tolerant ecotypes, generating a shift in the MC variants produced. At higher salinities (25 ppt), the selected ecotypes maintain their abundance but MC production decreases. The existence of MC-producing ecotypes adapted to different salinity conditions is relevant in determining the success of MAC organisms in a wide range of ecosystems and poses a risk to estuarine health, justifying further research.","PeriodicalId":94320,"journal":{"name":"Harmful algae","volume":"149 ","pages":"102936"},"PeriodicalIF":4.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145042206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of peroxide-based compounds on growth and toxicity of Microcystis aeruginosa. 过氧化物基化合物对铜绿微囊藻生长和毒性的影响。

IF 4.5

Harmful algae Pub Date : 2025-11-01 Epub Date: 2025-08-05 DOI: 10.1016/j.hal.2025.102930

Miquel Lürling, Ziwei Liao, Li Kang, West M Bishop, Maíra Mucci

{"title":"Effects of peroxide-based compounds on growth and toxicity of Microcystis aeruginosa.","authors":"Miquel Lürling, Ziwei Liao, Li Kang, West M Bishop, Maíra Mucci","doi":"10.1016/j.hal.2025.102930","DOIUrl":"10.1016/j.hal.2025.102930","url":null,"abstract":"Cyanobacterial blooms are increasing worldwide, compromising water bodies and ecosystem services. Among the possible management measures are curative methods, such as hydrogen peroxide, that can suppress cyanobacteria growth. However, the rapid breakdown of H2O2 could limit its effectiveness; thus new formulations (liquid and solid) that are, in theory, more stable have been developed. Here, we tested three new formulations (Oximycin™ P5, Phycomycin® SCP and Lake Guard® Oxy) and traditional peroxides (H2O2 and CaO2) on their capacity to end Microcystis aeruginosa bloom and their effect on microcystin dynamics. In general, all the products controlled M. aeruginosa biomass. The products were comparable mainly in the tests with lower initial Chl-a (150 µg L-1), with slightly less effectiveness of CaO2. At a higher initial bloom concentration (600 µg Chl-a L-1), all the products were effective in reducing Chl-a concentration to zero at a dose of 3 mg H2O2 L-1, except Lake Guard® Oxy and CaO2, which showed an increase in Chl-a by the end of the experiment. This suggests that OximycinTM P5, Phycomycin® SCP, and liquid H2O2 may perform better at lower doses. Nonetheless, at the highest dose tested (10 mg H2O2 L-1), all products were highly effective, with no signs of recovery. After one day of exposure, extracellular MC concentrations were higher in all treatments but reduced to lower levels than the control after nine days, pointing to the degradation of dissolved MCs. There is no obvious top choice in terms of overall efficiency. However, each product may be better suited for different purposes depending on the specific situation and strategy.","PeriodicalId":94320,"journal":{"name":"Harmful algae","volume":"149 ","pages":"102930"},"PeriodicalIF":4.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145042137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving antistress capacity and lipid productivity in the green alga Chlorella pyrenoidosa by adding abscisic acid under salt stress conditions 盐胁迫条件下添加脱落酸提高绿藻蛋白核小球藻的抗胁迫能力和脂质产量

Harmful algae Pub Date : 2024-09-15 DOI: 10.4490/algae.2024.39.8.28

Ke Ding, Zheng Chen, Qiwu Wan, Xiaolin Hu, Xiaohui Wang, Heng Li, Yongzhong Wang, Yang Luo, Debing Xiang

{"title":"Improving antistress capacity and lipid productivity in the green alga Chlorella pyrenoidosa by adding abscisic acid under salt stress conditions","authors":"Ke Ding, Zheng Chen, Qiwu Wan, Xiaolin Hu, Xiaohui Wang, Heng Li, Yongzhong Wang, Yang Luo, Debing Xiang","doi":"10.4490/algae.2024.39.8.28","DOIUrl":"https://doi.org/10.4490/algae.2024.39.8.28","url":null,"abstract":"In this study, the regulation of abscisic acid (ABA) on cell growth and lipid biosynthesis was investigated under saltinduced stress in Chlorella pyrenoidosa. It is found that as suffering from only salt stress, although the lipid content of single cell was improved, the inhibitory effects of stress on cell proliferation was visible. When the algal cells were exposed to salt stress and ABA conditions, lipid productivity was increased (45.35 mg L-1 d-1) by 1.17-fold compared to that of control cells (20.91 mg L-1 d-1), and the inhibition to cell growth was relieved. Transcriptomic analysis revealed that after adding ABA, these genes involved in antioxidant activity, jasmonic acid (JA) biosynthesis, and lipid biosynthesis were upregulated. Subsequently, we observed that the levels of glutathione, total antioxidant capacity, trehalose, and JA were elevated and the levels of reactive oxygen species were reduced. This study presents an effective approach to improve lipid production in algal cells, a new mechanism on that ABA alleviates intracellular oxidative stress through JA signaling pathway was elaborated.","PeriodicalId":94320,"journal":{"name":"Harmful algae","volume":"39 3","pages":"207-222"},"PeriodicalIF":0.0,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147896233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7