Topics in companion animal medicine最新文献

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Serum osteopontin level is elevated in dogs with mammary carcinomas. 犬乳腺癌患者血清骨桥蛋白水平升高。

IF 1.3

Topics in companion animal medicine Pub Date : 2025-09-29 DOI: 10.1016/j.tcam.2025.101024

Ramandeep Kaur, B V Sunil Kumar, Kuldip Gupta, Ashwani Kumar

{"title":"Serum osteopontin level is elevated in dogs with mammary carcinomas.","authors":"Ramandeep Kaur, B V Sunil Kumar, Kuldip Gupta, Ashwani Kumar","doi":"10.1016/j.tcam.2025.101024","DOIUrl":"https://doi.org/10.1016/j.tcam.2025.101024","url":null,"abstract":"The objective of the current research was to assess osteopontin (OPN) as a biomarker of canine mammary tumors (CMT). Twenty CMT tissues were collected post-surgery. Blood samples were also collected from the same dogs as well as healthy tumor free dogs and serum was harvested. Fragment encoding the immunodominant region of canine osteopontin (693 bp) was cloned and expressed as a HISx6 - tagged protein in a prokaryotic expression system using pPROEX-HTa vector. A 26 kDa recombinant osteopontin (rOPN) was expressed, purified by Ni-NTA affinity chromatography and assessed by SDS-PAGE and western blot analysis. In order to optimize a sandwich ELISA for the relative assessment of circulating OPN in the sera of dogs with mammary tumors, hyperimmune sera were produced against the expressed protein in rabbits and mice. The developed sELISA was found to be 90% specific and 85% sensitive with respect to histopathological examination in detecting CMT. The circulating levels of OPN were significantly elevated in dogs with mammary tumors as compared to the healthy animals. The results suggest that OPN can serve as a serum biomarker of CMT.","PeriodicalId":94254,"journal":{"name":"Topics in companion animal medicine","volume":" ","pages":"101024"},"PeriodicalIF":1.3,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful physiotherapy management of a geriatric dog with femoral head ostectomy (FHNO) and concurrent posterior contralateral limb amputation. 老年犬股骨头截骨术并发后对侧肢体截肢的物理治疗成功。

IF 1.3

Topics in companion animal medicine Pub Date : 2025-09-27 DOI: 10.1016/j.tcam.2025.101022

Mădălina Florina Gherman Dragomir, Anita Fehér, Iosif Vasiu, Daniela Neagu, William McCartney, Ciprian Andrei Ober

{"title":"Successful physiotherapy management of a geriatric dog with femoral head ostectomy (FHNO) and concurrent posterior contralateral limb amputation.","authors":"Mădălina Florina Gherman Dragomir, Anita Fehér, Iosif Vasiu, Daniela Neagu, William McCartney, Ciprian Andrei Ober","doi":"10.1016/j.tcam.2025.101022","DOIUrl":"10.1016/j.tcam.2025.101022","url":null,"abstract":"The current case aims to report the physiotherapy management and long-term outcomes of femoral head and neck ostectomy (FHNO) performed on a geriatric dog with pelvic-contralateral limb amputation. A 10-year-old, 36 kg mixed-breed castrated male dog with an amputated left hind limb was referred to our department for pain and lameness in the right hind limb. The dog was diagnosed with end-stage hip osteoarthritis (OA) and required an FHNO, as total hip replacement (THR) was not pursued due to financial constraints. Two weeks after the surgical intervention, the dog was still experiencing difficulty moving without support. Therefore, a physiotherapy protocol was initiated, consisting of weekly hydrotherapy sessions to promote muscle growth and strength, zonal laser therapy to manage joint pain, and systemic administration of nonsteroidal anti-inflammatory drugs (NSAIDs), nutraceutical joint supplements, and oral cannabis oil. Hydrotherapy was associated with a 5 cm increase in thigh circumference after eight sessions, indicating improved muscle mass and strength. After two months of physiotherapy, the dog showed considerable improvement and was able to walk independently. The owner was pleased with the long-term outcome. Physiotherapy led to positive clinical outcomes, including improved muscle strength and gait, with a favorable prognosis after 14 months of follow-up. Physiotherapy should be considered in dogs with severe hip OA managed by FHNO when the contralateral pelvic limb is amputated.","PeriodicalId":94254,"journal":{"name":"Topics in companion animal medicine","volume":" ","pages":"101022"},"PeriodicalIF":1.3,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0