Reproductive biology最新文献

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Changes in caprine cervical ERα, EP2, and EP4 expression among follicular, luteal phases of estrous cycle and early pregnancy. 孕早期、卵泡期和黄体期山羊宫颈ERα、EP2和EP4表达的变化

Reproductive biology Pub Date : 2026-10-15 DOI: 10.1016/j.repbio.2026.101294

Supapit Kanthawat, Saritvich Panyaboriban, Kongkiat Srisuwatanasagul, Fueangrat Thatsanabunjong, Sayamon Srisuwatanasagul

{"title":"Changes in caprine cervical ERα, EP2, and EP4 expression among follicular, luteal phases of estrous cycle and early pregnancy.","authors":"Supapit Kanthawat, Saritvich Panyaboriban, Kongkiat Srisuwatanasagul, Fueangrat Thatsanabunjong, Sayamon Srisuwatanasagul","doi":"10.1016/j.repbio.2026.101294","DOIUrl":"https://doi.org/10.1016/j.repbio.2026.101294","url":null,"abstract":"It is known that estradiol upregulates its local receptor (ERα) to prime the reproductive tract; however, how this estrogen signal interacts with specific EP receptor subtypes to regulate the physical flexibility remains unclear. This study investigated the spatial distribution and molecular expression of estrogen receptor alpha (ERα), and prostaglandin E2 receptor subtype 2 (EP2) and 4 (EP4) in the caprine cervix during the follicular, luteal, and early pregnancy stages. Cervical tissues from does were categorized into these three stages. The immunolocalization and protein expression of ERα, EP2, and EP4 were assessed using immunohistochemistry and Western blot analysis. Additionally, the relative mRNA expression of ESR1, PTGER2, and PTGER4 was evaluated by RT-qPCR. Results demonstrated a synchronized ERα-EP4 signaling axis during the follicular stage, with peak overall protein expression in the epithelium and smooth muscle. Post-ovulation, ERα and EP4 expression significantly declined, whereas epithelial EP2 was observed to be abundant during the luteal phase. During early pregnancy, ERα and EP4 protein levels dropped to their lowest points. Notably, a major disconnect occurred between gene activity and protein production; while the PTGER4 gene was highly active, EP4 protein remained minimal. Within the receptors examined, these findings indicate that the caprine cervix regulates ERα, EP2, and EP4 in a compartment-specific manner across the reproductive cycle. The combination of ERα and EP4 prepares the tissue for mating, whereas EP2 secures the barrier after ovulation. During early pregnancy, inhibiting the synthesis of EP4 protein serves as a crucial mechanism to avoid premature cervical dilation.","PeriodicalId":94190,"journal":{"name":"Reproductive biology","volume":" ","pages":"101294"},"PeriodicalIF":0.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149198590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

4D-DIA proteomics unveils the dual engines of human sperm capacitation. 4D-DIA蛋白质组学揭示了人类精子获能的双重引擎。

Reproductive biology Pub Date : 2026-10-15 DOI: 10.1016/j.repbio.2026.101290

Yu Lei, Hong Xie, Yuhong Zhao, Li Wang, Xia Bai, Min Jiang, Weiwei Zhi, Ling Yu, Yijuan Ding, Li Li, Zhu Chen, Li Wu, Jing Liu, Weixin Liu, Guohui Zhang

{"title":"4D-DIA proteomics unveils the dual engines of human sperm capacitation.","authors":"Yu Lei, Hong Xie, Yuhong Zhao, Li Wang, Xia Bai, Min Jiang, Weiwei Zhi, Ling Yu, Yijuan Ding, Li Li, Zhu Chen, Li Wu, Jing Liu, Weixin Liu, Guohui Zhang","doi":"10.1016/j.repbio.2026.101290","DOIUrl":"https://doi.org/10.1016/j.repbio.2026.101290","url":null,"abstract":"The systemic molecular regulatory landscape of sperm capacitation remains incompletely understood. This study utilized Four-dimensional independent data acquisition (4D-DIA) high-throughput quantitative proteomics to systematically analyze the global proteomic changes in healthy human sperm before and after in vitro capacitation. A total of 5812 quantifiable proteins were identified, of which 201 were significantly differentially expressed (Fold Change > 1.5, P < 0.05). Functional analysis revealed that upregulated proteins were highly enriched in core energy metabolism pathways, such as oxidative phosphorylation and the mitochondrial respiratory chain, confirming the driving role of an \"energy burst\" in capacitation. Furthermore, processes including RNA processing and translation, as well as post-translational modifications, were significantly activated, and the interaction network demonstrated a tight coupling between energy metabolism and translational machinery components. Concurrently, this study identified a group of potential novel regulators (e.g., TMEM89, TEX29) with high testis-specific expression that are upregulated post-capacitation. This study provides the first systematic mapping of the multidimensional proteomic dynamics during sperm capacitation, offering novel molecular biomarkers and potential targets for the etiological diagnosis of male infertility and assisted reproductive interventions.","PeriodicalId":94190,"journal":{"name":"Reproductive biology","volume":" ","pages":"101290"},"PeriodicalIF":0.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149198673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to: "Effect of ginger extract and/or fenofibrate on the associated infertility of experimentally induced obese Wistar Albino male rats" [Reprod Biol 26 (2): 101208]. “生姜提取物和/或非诺贝特对实验性诱导肥胖Wistar白化雄性大鼠相关不育的影响”[生殖生物学26(2):101208]的更正。

Reproductive biology Pub Date : 2026-08-21 DOI: 10.1016/j.repbio.2026.101273

Mohamed H Elsayed, Samia M Abd El-Rheem, Essam A Almadaly, Azza M Elkattawy, Nasr El Nasr, Doaa A Dorghamm, Walied S Abdo, Mona Assas, Tarek K Abouzed, Wael B El-Domany

引用次数: 0