Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)最新文献

{"title":"Dietary salicylates affect calcium and magnesium status in preeclampsia model rats induced by NG-nitro-L-arginine-methyl ester (L-NAME).","authors":"Rafsan Syabani Cholik, Katarzyna Skrypnik, Marta Karaźniewicz-Łada, Agnieszka Waśkiewicz, Joanna Suliburska","doi":"10.1016/j.jtemb.2025.127711","DOIUrl":"10.1016/j.jtemb.2025.127711","url":null,"abstract":"<p><p>Preeclampsia (PE) represents a complex hypertensive disorder of pregnancy which combines blood pressure elevation with organ dysfunction through mineral metabolism disturbances. The current guidelines recommend low-dose aspirin for PE prevention yet the impact of dietary salicylates on mineral status remains an open question. This research investigated how dietary salicylates and aspirin affect calcium and magnesium homeostasis in rats with NG-nitro-L-arginine-methyl ester (L-NAME)-induced preeclampsia. The research involved pregnant Sprague Dawley rats who received six different treatment groups: CH (healthy controls) and PE (preeclamptic controls) alongside four intervention groups that combined L-NAME with low or high dietary salicylates (LSP, HSP) or aspirin (LAP, HAP). The preeclampsia model used L-NAME (0.5 mg/mL) throughout gestational days 6-19. The flame atomic absorption spectrometry method measured calcium and magnesium levels in tissues and serum samples. The high-performance liquid chromatography (HPLC) and the ultra-high performance liquid chromatography-tandem mass spectrometry system (UHPLC-MS/MS) analyzed salicylic acid concentrations. The administration of L-NAME resulted in elevated blood pressure together with elevated calcium levels in placental and renal tissues. The tissue magnesium (Mg) levels increased in heart and brain tissues when animals consumed salicylates or aspirin without any effect on blood pressure. The combination of high salicylate intake resulted in reduced calcium (Ca) levels in femoral tissues and decreased albumin excretion in urine. The Ca:Mg molar ratio changed significantly in kidney tissue as well as placenta tissue and pancreas tissue. The preeclampsia condition caused by L-NAME disrupts the normal distribution of calcium and magnesium throughout maternal and fetal body tissues.</p>","PeriodicalId":94118,"journal":{"name":"Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)","volume":"91 ","pages":"127711"},"PeriodicalIF":3.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144805531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorative mechanism of dietary vitamin d and magnesium on newborn's pulmonary toxicity induced by cadmium.","authors":"Paria Amanpour, Zohre Eftekhari, Akram Eidi, Parvin Khodarahmi","doi":"10.1016/j.jtemb.2024.127469","DOIUrl":"https://doi.org/10.1016/j.jtemb.2024.127469","url":null,"abstract":"<p><p>Cadmium (Cd) exposure in mothers can cause respiratory issues in newborns, but the exact toxicity mechanisms are not fully understood. Vitamin D deficiency in Cd-exposed rats is associated with increased cadmium accumulation in tissues. Finding a cost-effective medication that is vital for the body while also reducing the effects of poisoning is crucial in treating poisonings. To investigate the mechanisms of Cd-induced lung toxicity, we examined the impact of prolonged Cd exposure in female rats before pregnancy on newborn lung health, focusing on sera TNF-α level, lung P53, Foxo1 mRNA, and lung VEGF, and BMP-4 protein level. A total of 50 rats were divided into control, Cd, Cd+Vitamin D, Cd+Mg, and Cd + Vitamin D+Mg groups. Cd exposure resulted in higher serum TNF-α levels and a significant rise in P53 mRNA levels. Additionally, the occurrence of hemorrhage, inflammatory cell infiltration, and thickening of alveolar walls decreased following treatment with vitamin D + Mg. Although Cd did not affect the newborns' body weight, it did impair their lung function. These findings suggest that the Cd-induced increase in the P53 gene expression could be alleviated by vitamin D and Mg, along with the elevation of VEGF and BMP-4 proteins and Foxo1 gene expression. The study revealed that environmental toxins can sometimes harm molecules and proteins, leading to damage in critical fetal tissues. However, these issues can be mitigated through essential supplements. STRUCTURED ABSTRACT: The increasing role of Cd in the erratic behavior of numerous biological and molecular entities, notably the development of fetal lung tissue, has made it beneficial to investigate the possible adverse effects of Cd exposure in pregnant mothers and fetal organ development, where instinctive molecular events occur. Researchers are encouraged to create new aspects of medications to reduce clinical symptoms and improve the quality of life due to exposure to metal toxins, particularly in industrialized countries. The present study aimed to evaluate histopathological and molecular modifications of fetal lungs caused by maternal Cd toxicosis and evaluate the possible ameliorating effects of vitamin D and Mg alone and in combination with fetal lung developmental abnormalities, followed by maternal toxin induction, which can be generalized to humans. Fifty female Wistar rats were purchased from the Pasteur Institute of Iran. To induce the model, cadmium at a dose of 2 mg/kg body weight was injected intraperitoneally into the female rats over 28 days before mating (5 days after injection in a week). Afterward, the female rats were randomly divided into type IV polycarbonate cages and mated with healthy male rats. The pregnancy was confirmed by observation of the vaginal plaque, which was subsequently observed, and the number of days of embryo formation was calculated. Subsequently, the pregnant rats were assigned to the following groups and received PBS, vitamin D, Mg, or vitam","PeriodicalId":94118,"journal":{"name":"Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)","volume":"84 ","pages":"127469"},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140961232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}