Carolyn Y Dadabay, Paula Barrientos Spaulding, Erick Valenzuela, Matthew Turner, Kaitlyn E Eckert, Riitta Julkunen-Tiitto, Nedra Noblit, Donald H Mansfield
{"title":"Polyphenols from the sagebrush <i>Artemisia tridentata</i> ssp. <i>tridentata</i> affect the redox state of cultured hepatocytes by direct and indirect mechanisms.","authors":"Carolyn Y Dadabay, Paula Barrientos Spaulding, Erick Valenzuela, Matthew Turner, Kaitlyn E Eckert, Riitta Julkunen-Tiitto, Nedra Noblit, Donald H Mansfield","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Basin big sagebrush (<i>Artemisia tridentata</i> Nutt. ssp. <i>tridentata</i> (Asteraceae)), is a widespread North American shrub which produces a variety of polyphenolic compounds. Although sagebrush has been used as a traditional remedy by natives and settlers to the region, the polyphenols in <i>Artemisia tridentata</i> ssp<i>. tridentata</i> have not been highly investigated for their bioactive properties. To determine whether these polyphenols affect the intracellular redox state, we measured their ability to neutralize radicals <i>in vitro</i> and in a human liver carcinoma cell line (HepG2), and their effects on intracellular glutathione levels. Extracts from <i>Artemisia tridentata</i> ssp. <i>tridentata</i> decreased the oxidation of 2'7'-dichlorofluorescin <i>in vitro</i> and in cultured cells. Cells treated with polyphenolic extracts showed increased levels of glutathione in a time and dose-dependent manner. Approximately 48 polyphenolic compounds were distinguishable in extracts, by HPLC/UV absorbance detection. Mass spectroscopy was used to identify thirteen compounds as aesculin, aesculetin, apigenin, apigenin-7-O-glucoside, axillarin, casticin, chlorogenic acid, isoscopoletin, kaempferol, luteolin, methyl-axillarin, quercetin, and scopoletin. These results indicate that polyphenols produced in <i>Artemisia tridentata</i> ssp. <i>tridentata</i> affect the redox state of living cells by multiple mechanisms.</p>","PeriodicalId":93740,"journal":{"name":"Current topics in phytochemistry","volume":"15 ","pages":"15-25"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258977/pdf/nihms-1632666.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40571089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}