Biophysics of Membrane Transport最新文献

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Membrane Biophysics: New Insights and Methods 膜生物物理学:新的见解和方法
Biophysics of Membrane Transport Pub Date : 2018-01-01 DOI: 10.1007/978-981-10-6823-2
Hongda Wang, Guohui Li
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引用次数: 1
Patch-clamp measurements of gap-junction channels in cultured cells 膜片钳测量培养细胞的缝隙连接通道
Biophysics of Membrane Transport Pub Date : 1992-01-01 DOI: 10.18419/OPUS-1962
D. Hülser, R. Eckert, G. Zempel, D. Paschke, Antonina Dunina-Barkovskaja
{"title":"Patch-clamp measurements of gap-junction channels in cultured cells","authors":"D. Hülser, R. Eckert, G. Zempel, D. Paschke, Antonina Dunina-Barkovskaja","doi":"10.18419/OPUS-1962","DOIUrl":"https://doi.org/10.18419/OPUS-1962","url":null,"abstract":"Direct intercellular communication in most tissues is made possible by proteinaceous pores called gap-junction channels. These channels bridge the extracellular gap between apposed cells and connect their intracellular compartments both electrically and metabolically. The extracellular parts of two hemichannels - the connexons - are linked thus forming a communicating gap-junction channel. A connexon is a hexamer of protein subunits which are members of the connexin family. Since connexin 32 (Cx32) was the first gap-junction channel protein to be sequenced from hepatocytes, it serves as a reference to which all other gap-junction proteins are compared. The individual channel conductance may vary between 25 and 150 pS. Gap-junction channels of some tissues are more voltage sensitive (e.g. liver) than others (e.g. heart). The question whether these differences in electrical properties may be attributed to the different connexins being expressed in these tissues is still unanswered. Several approaches to resolve this problem will be discussed in this contribution, all are based on double whole-cell patch-clamp measurements using isolated cell pairs, as follows: (1) Cells with two different channel conductances perfused with anti connexin antibodies to specifically block one channel species; (2) Cells with only one connexin species selected by immunological characterization; (3) Weakly coupled HeLa cells transfected with specific connexin genes, a method which resulted in better correlations between connexin type and single channel properties.","PeriodicalId":9326,"journal":{"name":"Biophysics of Membrane Transport","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91258185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of gap junctions by electrophysiological and electronmicroscopical methods 电生理和电子显微镜方法表征间隙连接
Biophysics of Membrane Transport Pub Date : 1990-01-01 DOI: 10.18419/OPUS-1948
D. Hülser, D. Paschke, F. Brümmer, R. Eckert
{"title":"Characterization of gap junctions by electrophysiological and electronmicroscopical methods","authors":"D. Hülser, D. Paschke, F. Brümmer, R. Eckert","doi":"10.18419/OPUS-1948","DOIUrl":"https://doi.org/10.18419/OPUS-1948","url":null,"abstract":"Gap junctions are ubiquitous in the animal kingdom from mesozoa to vertebrates. They must be discriminated from desmosomes which anchor cells together to form structural or functional units as well as from tight junctions which seal membranes of epithelial cells to each other so that the paracellular path becomes impermeable to molecules and a polarity of apical and basolateral surface is maintained.","PeriodicalId":9326,"journal":{"name":"Biophysics of Membrane Transport","volume":"544 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86965757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
X-ray studies on biological membranes using synchrotron radiation 同步辐射对生物膜的x射线研究
Biophysics of Membrane Transport Pub Date : 1988-01-01 DOI: 10.1007/BFB0111240
P. Laggner
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引用次数: 40
The insect sodium channel as the target for insect selective neurotoxins from scorpion venom 昆虫钠通道作为蝎子毒液中昆虫选择性神经毒素的靶点
Biophysics of Membrane Transport Pub Date : 1900-01-01 DOI: 10.1021/bk-1995-0591.ch004
E. Zlotkin, H. Moskowitz, R. Herrmann, M. Pelhate, D. Gordon
{"title":"The insect sodium channel as the target for insect selective neurotoxins from scorpion venom","authors":"E. Zlotkin, H. Moskowitz, R. Herrmann, M. Pelhate, D. Gordon","doi":"10.1021/bk-1995-0591.ch004","DOIUrl":"https://doi.org/10.1021/bk-1995-0591.ch004","url":null,"abstract":"","PeriodicalId":9326,"journal":{"name":"Biophysics of Membrane Transport","volume":"96 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82149427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
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