International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute最新文献

筛选

英文中文

Human β-defensin-2 gene transduction of dental pulp cells: A model for pulp antimicrobial gene therapy. 牙髓细胞的人类 β防御素-2 基因转导：牙髓抗菌基因治疗模型。

International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute Pub Date : 2004-01-01

George T-J Huang, Hai-Bo Zhang, Chunyi Yin, Sang Hyuk Park

{"title":"Human β-defensin-2 gene transduction of dental pulp cells: A model for pulp antimicrobial gene therapy.","authors":"George T-J Huang, Hai-Bo Zhang, Chunyi Yin, Sang Hyuk Park","doi":"","DOIUrl":"","url":null,"abstract":"The objective of this study was to determine whether cells from human pulp can be transduced to express the antimicrobial peptide--human β-defensin-2 (HBD-2). Primary human pulp cells and gingival fibroblasts from normal tissue, as well as two mouse cell lines (NIH 3T3 and AT-84) and a human cell line SCC-9 were transduced with a retroviral vector carrying HBD-2 cDNA. ELISA and Northern blot analyses were performed to detect HBD-2 expression by these transduced cells. Antimicrobail assays using recombinant HBD-2 were performed on two caries-associated bacteria Streptococcus mutnas and Lactobacillus acidophilus. The results showed that transduced pulp cells secreted 62.4 ± 27.2 ng/3 days of HBD-2, which was comparable to that by NIH 3T3 (78.0 ± 14.1 ng/4 days), and higher than those by gingival fibroblasts (17.9 ± 7.9 ng/3 days), AT-84 (2.6 ± 1.0 ng/3 days), and SCC-9 (47.6 ± 9.9 ng/3 days). Northern blot analysis showed that the levels of HBD-2 mRNA expression correlated with their protein secretion levels. There was approximately 50% reduction of growth when S. mutans and L. acidophilus were exposed to HBD-2 at 1 µM. Pulp cells appear to be suitable for HBD-2 transduction using retroviral vectors, suggesting a potential for use in controlling pulpal infections.","PeriodicalId":92222,"journal":{"name":"International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute","volume":"29 1","pages":"7-12"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812695/pdf/nihms892209.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35842442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0