Hematology & medical oncology最新文献

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Nanoparticles for hematologic diseases detection and treatment. 用于血液疾病检测和治疗的纳米颗粒。
Hematology & medical oncology Pub Date : 2019-06-28 DOI: 10.15761/hmo.1000183
Tania Limongi, Francesca Susa, Valentina Cauda
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引用次数: 6
Intra-osseous Co-transplantation of CD34-selected Umbilical Cord Blood and Mesenchymal Stromal Cells cd34选择脐带血与间充质间质细胞的骨内共移植
Hematology & medical oncology Pub Date : 2014-12-06 DOI: 10.15761/HMO.1000105
L. Metheny, S. Eid, Karen T. Lingas, J. Reese, H. Meyerson, Alexander A. Tong, M. de Lima, A. Huang
{"title":"Intra-osseous Co-transplantation of CD34-selected Umbilical Cord Blood and Mesenchymal Stromal Cells","authors":"L. Metheny, S. Eid, Karen T. Lingas, J. Reese, H. Meyerson, Alexander A. Tong, M. de Lima, A. Huang","doi":"10.15761/HMO.1000105","DOIUrl":"https://doi.org/10.15761/HMO.1000105","url":null,"abstract":"Human mesenchymal stromal cells (MSC) have been shown to support the growth and differentiation of hematopoietic stem cells (HSC). We hypothesized that intra-osseous (IO) co-transplantation of MSC and umbilical cord blood (UCB) may be effective in improving early HSC engraftment, as IO transplantation has been demonstrated to enhance UCB engraftment in NOD SCID-gamma (NSG) mice. Following non-lethal irradiation (300rads), 6 groups of NSG mice were studied: 1) intravenous (IV) UCB CD34+ cells, 2) IV UCB CD34+ cells and MSC, 3) IO UCB CD34+ cells, 4) IO UCB CD34+ cells and IO MSC, 5) IO UCB CD34+ cells and IV MSC, and 6) IV UCB CD34+ and IO MSC. Analysis of human-derived CD45+, CD3+, and CD19+ cells 6 weeks following transplant revealed the highest level of engraftment in the IO UCB plus IO MSC cohort. Bone marrow analysis of human CD13 and CD14 markers revealed no significant difference between cohorts. We observed that IO MSC and UCB co-transplantation led to superior engraftment of CD45+, CD3+ and CD19+ lineage cells in the bone marrow at 6 weeks as compared with the IV UCB cohort controls. Our data suggests that IO co-transplantation of MSC and UCB facilitates human HSC engraftment in NSG mice.","PeriodicalId":91691,"journal":{"name":"Hematology & medical oncology","volume":"46 1","pages":"25 - 29"},"PeriodicalIF":0.0,"publicationDate":"2014-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67469357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
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