Juan Guevara, Jamie Romo, Troy McWhorter, Natalia Valentinova Guevara
{"title":"Analogs of LDL Receptor Ligand Motifs in Dengue Envelope and Capsid Proteins as Potential Codes for Cell Entry.","authors":"Juan Guevara, Jamie Romo, Troy McWhorter, Natalia Valentinova Guevara","doi":"10.1155/2015/646303","DOIUrl":"https://doi.org/10.1155/2015/646303","url":null,"abstract":"<p><p>It is established that cell entry of low density lipoprotein particles (LLPs) containing Apo B100 and Apo E is mediated by receptors and GAGs. Receptor ligand motifs, X<b>BBB</b>XX<b>B</b>X, X<b>BB</b>X<b>B</b>X, and Ψ<b>B</b>ΨX<b>B</b>, and mono- and bipartite NLS sequences are abundant in Apo E and Apo B100 as well as in envelope and capsid proteins of Dengue viruses 1-4 (DENV1-4). Synthetic, fluorescence-labeled peptides of sequences in DENV2 envelope protein, and DENV3 capsid that include these motifs were used to conduct a qualitative assessment of cell binding and entry capacity using HeLa cells. DENV2 envelope peptide, Dsp2EP, <sup>0564</sup>Gly-Gly<sup>0595</sup>, was shown to bind and remain at the cell surface. In contrast, DENV3 capsid protein peptide, Dsp3CP, <sup>0002</sup>Asn-Gln<sup>0028</sup>, readily enters HeLa cells and accumulates at discrete loci in the nucleus. FITC-labeled dengue synthetic peptides colocalize with Low Density Lipoprotein-CM-DiI and Apo E-CM-DiI to a degree that suggests that Dengue viruses may utilize cell entry pathways used by LLPs.</p>","PeriodicalId":91568,"journal":{"name":"Journal of viruses","volume":"2015 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/646303","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34352603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}