Journal of stem cell research and transplantation最新文献

Checkpoint Inhibitor Pneumonitis Induced by Programmed Death-1 Antibody: A Case Report and Literature Review 程序性死亡-1抗体诱导的检查点抑制剂肺炎1例报告及文献复习

Journal of stem cell research and transplantation Pub Date : 2021-06-23 DOI: 10.26420/jstemcellrestransplant.2021.1039

Y. Zheng, J. Li, D. Chen, Y. Li, L. Dai, L. Huang, M. Wang

引用次数: 0

NPS2143 Modulates the Phenotypic Switching of PASMCs by Inhibiting Autophagy in Hypoxia-Induced Pulmonary Hypertension NPS2143通过抑制缺氧诱导的肺动脉高压自噬调节PASMCs的表型转换

Journal of stem cell research and transplantation Pub Date : 2021-06-16 DOI: 10.26420/jstemcellrestransplant.2021.1038

L. Wang, H. Shao, B. Che, N. Wang, X. Peng, C. Wei

{"title":"NPS2143 Modulates the Phenotypic Switching of PASMCs by Inhibiting Autophagy in Hypoxia-Induced Pulmonary Hypertension","authors":"L. Wang, H. Shao, B. Che, N. Wang, X. Peng, C. Wei","doi":"10.26420/jstemcellrestransplant.2021.1038","DOIUrl":"https://doi.org/10.26420/jstemcellrestransplant.2021.1038","url":null,"abstract":"Background and Objectives: Pulmonary Artery Hypertension (PAH) is considered as a malignant tumor in cardiovascular disease. Our previous study found that Calcium-Sensing Receptor (CaSR) is involved in pulmonary vascular remodeling in hypoxic pulmonary hypertension (HPH). However, the relationship of Pulmonary Artery Smooth Muscle Cell (PASMC) phenotypic switching, proliferation, and autophagy in CaSR-related HPH remain unclear. The purpose of this study was to detect the role of a CaSR antagonist, NPS2143, on the vascular remodeling by autophagy modulation under hypoxia. Methods: Hypoxic rat PAH model were simulated in vivo. Meanwhile, mean Pulmonary Artery Pressure (mPAP) was measured while RVI, WT%, and WA% indices were calculated. Immunohistochemistry and Western blot were used to detect phenotypic switching and cell proliferation in pulmonary arteriole. Cell viability was determined in vitro by CCK8 and cell cycle. Cell proliferation, phenotypic switching, autophagy level and PI3K/Akt/mTOR pathways were investigated in human PASMCs through mRNA or Western blot methods. Results: Rats with hypoxic-induced PAH had an increased mPAP, RVI, WT% and WA%. Moreover, expression of CaSR was significantly increased, followed by activation of autophagy (increased LC3b and decreased p62), phenotypic switching of PASMCs (reduced calponin, SMA-a and increased OPN) and pulmonary vascular remodeling. However, NPS2143 weakened these hypoxic effects. The results using hypoxic-induced human PASMCs confirmed that NPS2143 suppressed autophagy and reversed phenotypic switching in vitro by inhibiting PI3K/Akt/mTOR pathways. Conclusions: Our study demonstrates that NPS2143 was conducive to inhibit the proliferation and reverse phenotypic switching of PASMCs by regulating autophagy levels in HPH and vascular remodeling.","PeriodicalId":91561,"journal":{"name":"Journal of stem cell research and transplantation","volume":"324 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78007908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Bulky Dermoid Cyst of the Floor of the Mouth 口腔底部巨大的皮样囊肿

Journal of stem cell research and transplantation Pub Date : 2021-06-11 DOI: 10.26420/jstemcellrestransplant.2021.1037

S. Z, Y. H, G. L., G. H., G. H., Qiu C, C. H

引用次数: 0

Driver Gene Mutations and Risk of Lymph Node Metastasis in Non-Small Cell Lung Cancer 非小细胞肺癌的驱动基因突变与淋巴结转移风险

Journal of stem cell research and transplantation Pub Date : 2021-05-20 DOI: 10.26420/jstemcellrestransplant.2021.1035

Z Feiyue, Z. Li, Zhang Bing, Chen Benchao, Wan Shuting, Du-Juan Yu, L Gaofeng

{"title":"Driver Gene Mutations and Risk of Lymph Node Metastasis in Non-Small Cell Lung Cancer","authors":"Z Feiyue, Z. Li, Zhang Bing, Chen Benchao, Wan Shuting, Du-Juan Yu, L Gaofeng","doi":"10.26420/jstemcellrestransplant.2021.1035","DOIUrl":"https://doi.org/10.26420/jstemcellrestransplant.2021.1035","url":null,"abstract":"Non-Small Cell Lung Cancer (NSCLC) lymph node status is closely related to its diagnosis, treatment, and prognosis. The lymph node status is an important basis for formulating clinical treatment strategies of NSCLC, therefore comprehensive and profound understanding of risk factors for lymph node metastasis is essential. There are many known factors for lymph node metastasis in NSCLC, such as pathological subtypes, tumor size, tumor location. Meanwhile, whether the mutation of the driver gene affects the lymph node metastasis is still lacking enough research. This article aims to elaborate the relationship between NSCLC driver gene mutation and lymph node metastasis, from NSCLC lymph node metastasis-related risk factors, driver genes and lymph node metastasis, metastatic lymph node mutation status analysis and detection these several aspects to summarize the latest research progress in NSCLC driver gene mutation and lymph node metastasis risk. It also fully explained the correlation between driver gene mutations and NSCLC tumor biological behaviors such as lymph node metastasis.","PeriodicalId":91561,"journal":{"name":"Journal of stem cell research and transplantation","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90145540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem Cells Applications in Therapeutics and Site-Specific Genome Editing Through CRISPR Cas9 System 干细胞在治疗学中的应用以及通过CRISPR Cas9系统进行位点特异性基因组编辑

Journal of stem cell research and transplantation Pub Date : 2021-04-25 DOI: 10.26420/JSTEMCELLRESTRANSPLANT.2021.1036

Muhammad Javed Iqbal, Muhammad Mukheed, A. Khan, S. Irfan, Usama Hayat, Muhammad Umair Khalid, M. Raza, Kainat Amjad, Marriyam Tallat

{"title":"Stem Cells Applications in Therapeutics and Site-Specific Genome Editing Through CRISPR Cas9 System","authors":"Muhammad Javed Iqbal, Muhammad Mukheed, A. Khan, S. Irfan, Usama Hayat, Muhammad Umair Khalid, M. Raza, Kainat Amjad, Marriyam Tallat","doi":"10.26420/JSTEMCELLRESTRANSPLANT.2021.1036","DOIUrl":"https://doi.org/10.26420/JSTEMCELLRESTRANSPLANT.2021.1036","url":null,"abstract":"Stem cells are immature cells that have ability to differentiate into all specific and mature cells in body. The two main characteristics of stem cells are selfrenewable and ability to differentiate into all mature, functional and adult cells types. There are the two major classes a) pluripotent stem cells which have potential to differentiate in all adult cell and b) multipotent stem cells which have capacity to differentiate into many adult cells but not in all cell types. Due to the self-renewable ability stem cells are used in therapeutics, tissue regeneration, disease modeling, regenerative medicines and to treat cardiovascular diseases, neural disorders such as Parkinson’s disease and most importantly to treat carcinomas. The human induced pluripotent stem cells provide a great platform to study and treatment of human diseases because these are able to differentiate into many functional and specialized adult cells of body. The genome editing tools such as CRISPR Cas9 system and TALENs are used to generate multiple DNA variants in hPSCs by inducing site specific mutations, frame shift mutation and deletion. In present days CRISPR Cas9 is more efficient and frequent method for genome editing which is derived from bacterial cell.","PeriodicalId":91561,"journal":{"name":"Journal of stem cell research and transplantation","volume":"136 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82993911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Stem Cells Applications in Therapeutics and Site-Specific Genome Editing Through CRISPR Cas9 System 干细胞在治疗学中的应用以及通过CRISPR Cas9系统进行位点特异性基因组编辑

Journal of stem cell research and transplantation Pub Date : 2021-02-06 DOI: 10.26420/jstemcellrestransplant.2021.1033

Javed M, K. A, Mukheed M

{"title":"Stem Cells Applications in Therapeutics and Site-Specific Genome Editing Through CRISPR Cas9 System","authors":"Javed M, K. A, Mukheed M","doi":"10.26420/jstemcellrestransplant.2021.1033","DOIUrl":"https://doi.org/10.26420/jstemcellrestransplant.2021.1033","url":null,"abstract":"Stem cells ae immature cells that have ability to differentiate into all specific and mature cells in body. The two main characteristics of stem cells are selfrenewable and ability to differentiate into all mature, functional and adult cells types. There are the two major classes a) pluripotent stem cells which have potential to differentiate in all adult cell and b) multipotent stem cells which have capacity to differentiate into many adult cells but not in all cell types. Due to the self-renewable ability stem cells are use in therapeutics, tissue regeneration, disease modeling and regenerative medicines and to treat cardiovascular diseases, neural disorders such as Parkinson’s disease and most importantly to treat carcinomas. The human induced pluripotent stem cells provide a great platform to study and treatment of human diseases because these are able to differentiate into many functional and specialized adult cells of body. The genome editing tools such as CRISPR Cas9 system and TALENs are used to generate multiple DNA variants in hPSCs by inducing site specific mutations, frame shift mutation and deletion. In present days CRISPR Cas9 is more efficient and frequent method for genome editing which is derived from bacterial cell.","PeriodicalId":91561,"journal":{"name":"Journal of stem cell research and transplantation","volume":"29 12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82730277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pediatric Stroke and Cell-Based Treatment – Pivotal Role of Brain Plasticity 儿童中风和细胞治疗-脑可塑性的关键作用

Journal of stem cell research and transplantation Pub Date : 2019-05-04 DOI: 10.26420/JSTEMCELLRESTRANSPLANT.2019.1029

A. Jensen

{"title":"Pediatric Stroke and Cell-Based Treatment – Pivotal Role of Brain Plasticity","authors":"A. Jensen","doi":"10.26420/JSTEMCELLRESTRANSPLANT.2019.1029","DOIUrl":"https://doi.org/10.26420/JSTEMCELLRESTRANSPLANT.2019.1029","url":null,"abstract":"Pediatric cerebrovascular disorders like stroke are among the top 10 causes of death in children, with rates highest in the first year of life, while survivors may face lifelong sequelae, disability, and/or cerebral palsy for which there is no cure at present. Individual treatments using human autologous cord blood Mononuclear Cells (hucbMNC) containing stem cells have yielded promising results. However, stroke is an entity with heterogenic etiologies including arterial ischemic stroke, hemorrhagic stroke, hemorrhagic infarction, and sinus venous thrombosis in newborns, infants, children, and adults. Hence, any attempt to examine care, non-cellular or cell-based therapies has to merit the specific characteristics of this heterogeneity as far as age, symptoms, diagnostics, pathophysiology, histopathology, clinical course and prevalence are concerned. This review describes the various etiologies of stroke for the Neonatal/Perinatal and childhood subsets of the pediatric population as a basis for established non-cellular and novel cell-based therapeutic approaches using cord blood mononuclear cells in the preclinical and clinical setting. In addition, due to its fundamental importance for cell-based therapeutic strategies for stroke, an account of brain plasticity along with blood-brain barrier function and the distinctions between the developing brain and the adult brain is provided. It is concluded that on the present balance of evidence the pathophysiological characteristics associated with plasticity of the developing brain are closely linked to the pharmacological action of hucbMNC in such a way that cell-based treatment might be more efficacious in pediatric stroke than in adult stroke.","PeriodicalId":91561,"journal":{"name":"Journal of stem cell research and transplantation","volume":"229 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78902005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Adult Stem Cells and Diabetes Therapy. 成体干细胞与糖尿病治疗。

Journal of stem cell research and transplantation Pub Date : 2015-01-01 Epub Date: 2015-12-31

Handenur Ilgun, Joseph William Kim, LuGuang Luo

引用次数: 0