Lili Wang, Li He, Guoqiang Bao, Xin He, Saijun Fan, Haichao Wang
{"title":"Ionizing Radiation Induces HMGB1 Cytoplasmic Translocation and Extracellular Release.","authors":"Lili Wang, Li He, Guoqiang Bao, Xin He, Saijun Fan, Haichao Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>A nucleosomal protein, HMGB1, can be secreted by activated immune cells or passively released by dying cells, thereby amplifying rigorous inflammatory responses. In this study we aimed to test the possibility that ionizing radiation similarly induces cytoplasmic HMGB1 translocation and extracellular release.</p><p><strong>Method: </strong>Human skin fibroblast (GM0639) and bronchial epithelial (16HBE) cells and animals (rats) were exposed to X-ray radiation, and HMGB1 translocation and release were assessed by immunocytochemistry and immunoassay, respectively.</p><p><strong>Results: </strong>At a wide dose range (4.0 - 12.0 Gy), X-ray radiation induced a dramatic cytoplasmic HMGB1 translocation, and triggered a time- and dose-dependent HMGB1 release both <i>in vitro</i> and <i>in vivo</i>. The radiation-mediated HMGB1 release was associated with noticeable chromosomal DNA damage and loss of cell viability.</p><p><strong>Conclusion: </strong>radiation induces HMGB1 cytoplasmic translocation and extracellular release through active secretion and passive leakage processes.</p>","PeriodicalId":91486,"journal":{"name":"Guo ji fang she yi xue he yi xue za zhi = International journal of radiation medicine and nuclear medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911189/pdf/nihms790640.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34601359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}