Expert opinion on environmental biology最新文献

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Climate change and the migration of the Southern population of Madagascar 气候变化和马达加斯加南部人口的迁移
Expert opinion on environmental biology Pub Date : 2019-01-01 DOI: 10.4172/2325-9655-c1-045
Fanja T Ralinirina, J. Ravalison
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引用次数: 0
Effects of climate change on Brazil’s sugarcane agroecological zoning 气候变化对巴西甘蔗农业生态区划的影响
Expert opinion on environmental biology Pub Date : 2019-01-01 DOI: 10.4172/2325-9655-C1-044
M. Caldas, Gabriel Granco, P. D. M. Júnior
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引用次数: 0
Land loss and sea transgression due to glacial recession under climate warming in the Arctic 北极气候变暖下冰川消退导致的土地流失和海洋海侵
Expert opinion on environmental biology Pub Date : 2019-01-01 DOI: 10.4172/2325-9655-C1-043
W. Ziaja
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引用次数: 0
A High Concentration of Genistein Induces Cell Death in Human Uterine Leiomyoma Cells by Autophagy. 高浓度染料木素诱导人子宫平滑肌瘤细胞自噬死亡。
Expert opinion on environmental biology Pub Date : 2016-01-01 Epub Date: 2016-05-30 DOI: 10.4172/2325-9655.S1-003
Lysandra Castro, Xioahua Gao, Alicia B Moore, Linda Yu, Xudong Di, Grace E Kissling, Darlene Dixon
{"title":"A High Concentration of Genistein Induces Cell Death in Human Uterine Leiomyoma Cells by Autophagy.","authors":"Lysandra Castro,&nbsp;Xioahua Gao,&nbsp;Alicia B Moore,&nbsp;Linda Yu,&nbsp;Xudong Di,&nbsp;Grace E Kissling,&nbsp;Darlene Dixon","doi":"10.4172/2325-9655.S1-003","DOIUrl":"https://doi.org/10.4172/2325-9655.S1-003","url":null,"abstract":"<p><p>Genistein, an estrogenic, soy-derived isoflavone, may play a protective role against hormone-related cancers. We have reported that a high concentration of genistein inhibits cell proliferation and induces apoptosis in human uterine smooth muscle cells, but not in leiomyoma (fibroid) cells. To better understand the differential cell death responses of normal and tumor cells to a high concentration of genistein, we treated uterine smooth muscle cells and uterine leiomyoma cells with 50 μg/ml of genistein for 72 h and 168 h, and assessed for mediators of apoptosis, cytotoxicity and autophagy. We found that leiomyoma cells had increased protection from apoptosis by expressing an increased ratio of Bcl-2: bak at 72 h and 168 h; however, in smooth muscle cells, the Bcl-2: bak ratio was decreased at 72 h, but significantly rebounded by 168 h. The apoptosis extrinsic factors, Fas ligand and Fas receptor, were highly expressed in uterine smooth muscle cells following genistein treatment at both time points as evidenced by confocal microscopy. This was not seen in the uterine leiomyoma cells; however, cytotoxicity as indicated by elevated lactate dehydrogenase levels was significantly enhanced at 168 h. Increased immunoexpression of an autophagy/autophagosome marker was also observed in the leiomyoma cells, although minimally present in smooth muscle cells at 72 h. Ultrastructurally, there was evidence of autophagic vacuoles in the leiomyoma cells; whereas, the normal smooth muscle cells showed nuclear fragmentation indicative of apoptosis. In summary, our data show differential cell death pathways induced by genistein in tumor and normal uterine smooth muscle cells, and suggest novel cell death pathways that can be targeted for preventive and intervention strategies for inhibiting fibroid tumor cell growth <i>in vivo</i>.</p>","PeriodicalId":91480,"journal":{"name":"Expert opinion on environmental biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976942/pdf/nihms-802863.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34746456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
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