{"title":"Calcium Signaling","authors":"M. Islam, J. Bruton, A. Cheng, H. Westerblad","doi":"10.1007/978-3-030-12457-1","DOIUrl":"https://doi.org/10.1007/978-3-030-12457-1","url":null,"abstract":"","PeriodicalId":91460,"journal":{"name":"Calcium signaling (Santa Clara, Calif.)","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77186766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Phase Lag between Agonist-Induced Oscillatory Ca<sup>2+</sup> and IP<sub>3</sub> Signals Does Not Imply Causality (December 2015).","authors":"Pei-Chi Yang, M Saleet Jafri","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Activated phospholipase C (PLC*) generates 1,4,5-triphosphate (IP<sub>3</sub>) and diacylglycerol (DAG) from phosphatidyl inositol (PIP<sub>2</sub>). The DAG remains in the plasma membrane and co-activates conventional protein kinase C (PKC) with Ca<sup>2+</sup>. We have developed a mathematical model for the activation of the Ca<sup>2+</sup>-dependent PKC and its negative feedback on phospholipase C (PLC) and coupled it to the De Young-Keizer model for IP<sub>3</sub> mediated Ca<sup>2+</sup> oscillations. The model describes the cascade of reactions for the translocation of PKC to plasma membrane, and simulates activation of Ca<sup>2+</sup> and diacylglycerol (DAG) oscillations. The model demonstrates that oscillations in Ca<sup>2+</sup> and DAG are possible with or without a positive Ca<sup>2+</sup> feedback on phospholipase C consistent with experiment. In many experimental studies, the timing of the peaks of the Ca<sup>2+</sup> and IP<sub>3</sub> oscillations have been used to suggest causality, i.e. that the IP<sub>3</sub> oscillations cause the Ca<sup>2+</sup> oscillations. The model is used to explore this question. To this end, the positive and negative feedback between Ca<sup>2+</sup> and IP<sub>3</sub> production are modulated, resulting in changes to the phase lag between the peaks in [Ca<sup>2+</sup>]<sub>cyt</sub> and [IP]<sub>cyt</sub>. The model simulates a possible experimental protocol that can be used to differentiate whether or not the positive feedback of Ca<sup>2+</sup> on PLC is needed for the oscillations.</p>","PeriodicalId":91460,"journal":{"name":"Calcium signaling (Santa Clara, Calif.)","volume":"2 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874533/pdf/nihms783428.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34512655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}