Journal of stem cell and regenerative biology最新文献

Reconstruction of Lower Jaw by Iliac Bone Graft, Experimental Study on Rabbit and Role of Mesenchymal Stem Cells 兔髂骨移植重建下颌骨的实验研究及间充质干细胞的作用

Journal of stem cell and regenerative biology Pub Date : 2018-06-14 DOI: 10.15436/2471-0598.18.1879

R. Kummoona, Ali Zayed, Ommega Internationals

{"title":"Reconstruction of Lower Jaw by Iliac Bone Graft, Experimental Study on Rabbit and Role of Mesenchymal Stem Cells","authors":"R. Kummoona, Ali Zayed, Ommega Internationals","doi":"10.15436/2471-0598.18.1879","DOIUrl":"https://doi.org/10.15436/2471-0598.18.1879","url":null,"abstract":"page no: 20/24 www.ommegaonline.org Vol:4 Issue: 1 Abstract: In this research, twelve young male Rabbits age of 3 months are weighted 1.25 kg, divided in two groups of 5 Rabbits and 2 Rabbits were used as control. They were subjected to surgical osteoctomy by excising bone 1.5cm from the body of the mandible and bone graft of 2cm harvested from the iliac crest. The graft was fixed by soft stainless steel wire of 0.25 mm, post-operative systemic antibiotic of ceftriaxone of 20 mg / kg IM of body weight for six days prescribed, oxidized regenerated cellulose mesh was used to cover the graft of group B. After 3 months the experiment was terminated, the result were 2 Rabbits passed, one Rabbit end with infection and one with slight injury to Sciatic nerve without effect on mastication. The dyed Rabbits and one with infection were excluded from this experiment. The cytological changes of bone grafting showed formation of healthy granulation tissue with mesenchymal stem cells derived from bone marrow of bony segments of the mandible with platelets growth factor (PDGF) and from periosteum and covering muscles with large amount of fibroblasts with tiny blood vessels. Osteoblast was noticed more with chondrocyte and osteoid tissue with bone graft that covered by Surgical mesh (oxidized regenerated cellulose) soaked in blood and covered bone graft of group B to get faster healing in group B than in group A. The aim of this research to understand the cytological changes that occurred in the graft and recipient area.","PeriodicalId":91343,"journal":{"name":"Journal of stem cell and regenerative biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88536123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Immunomodulation by Mesenchymal Stromal Cells and Their Clinical Applications. 间充质基质细胞的免疫调节作用及其临床应用。

Journal of stem cell and regenerative biology Pub Date : 2017-01-01 Epub Date: 2017-04-10 DOI: 10.15436/2471-0598.17.022

Joaquin Cagliani, Daniel Grande, Ernesto P Molmenti, Edmund J Miller, Horacio L R Rilo

引用次数: 0

Neurons Self-Organize Around Salivary Epithelial Cells in Novel Co-Culture Model 神经元在唾液上皮细胞周围自组织的新共培养模型

Journal of stem cell and regenerative biology Pub Date : 2016-05-27 DOI: 10.15436/2471-0598.16.013

S. Sommakia, O. Baker

引用次数: 6

Mice lacking MKP-1 and MKP-5 Reveal Hierarchical Regulation of Regenerative Myogenesis. 小鼠缺乏MKP-1和MKP-5揭示再生肌发生的分级调控。

Journal of stem cell and regenerative biology Pub Date : 2015-11-12 DOI: 10.15436/2741-0598.15.005

Hao Shi, Florian Gatzke, Julia M Molle, Han Bin Lee, Emma T Helm, Jessie J Oldham, Lei Zhang, David E Gerrard, Anton M Bennett

{"title":"Mice lacking MKP-1 and MKP-5 Reveal Hierarchical Regulation of Regenerative Myogenesis.","authors":"Hao Shi, Florian Gatzke, Julia M Molle, Han Bin Lee, Emma T Helm, Jessie J Oldham, Lei Zhang, David E Gerrard, Anton M Bennett","doi":"10.15436/2741-0598.15.005","DOIUrl":"https://doi.org/10.15436/2741-0598.15.005","url":null,"abstract":"<p><p>The relative contribution of the MAP kinase phosphatases (MKPs) in the integration of MAP kinase-dependent signaling during regenerative myogenesis has yet to be fully investigated. MKP-1 and MKP-5 maintain skeletal muscle homeostasis by providing positive and negative effects on regenerative myogenesis, respectively. In order to define the hierarchical contributions of MKP-1 and MKP-5 in the regulation of regenerative myogenesis we genetically ablated both MKPs in mice. MKP-1/MKP 5-deficient double-knockout (MKP1/5- DKO) mice were viable, and upon skeletal muscle injury, were severely impaired in their capacity to regenerate skeletal muscle. Satellite cells were fewer in number in MKP1/5-DKO mice and displayed a reduced proliferative capacity as compared with those derived from wild-type mice. MKP1/5-DKO mice exhibited increased inflammation and the macrophage M1 to M2 transition during the resolution of inflammation was impaired following injury. These results demonstrate that the actions of MKP-1 to positively regulate myogenesis predominate over those of MKP-5, which negatively regulates myogenesis. Hence, MKP-1 and MKP-5 function to maintain skeletal muscle homeostasis through non-overlapping and opposing signaling pathways.</p>","PeriodicalId":91343,"journal":{"name":"Journal of stem cell and regenerative biology","volume":"1 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2015-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822085/pdf/nihms744609.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34390173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Gene Editing in Human Pluripotent Stem Cells: Choosing the Correct Path 人类多能干细胞的基因编辑:选择正确的路径

Journal of stem cell and regenerative biology Pub Date : 2015-11-05 DOI: 10.15436/2471-0598.15.004

Amar M. Singh, Valeriya V Adjan Steffey, Tseten Yeshi, D. Allison

{"title":"Gene Editing in Human Pluripotent Stem Cells: Choosing the Correct Path","authors":"Amar M. Singh, Valeriya V Adjan Steffey, Tseten Yeshi, D. Allison","doi":"10.15436/2471-0598.15.004","DOIUrl":"https://doi.org/10.15436/2471-0598.15.004","url":null,"abstract":"The recent emergence of targeted nucleases has opened up new opportunities for performing genetic modifications with human pluripotent stem cells (hPSCs). These modifications can range from the creation of a routine knock-out to the more challenging single point-mutation. For both the new and established user, deciding on the best approach for the specific modification of interest can be an arduous task, as new and improved technologies are rapidly and continuously being developed. The choices between the reagents and methodologies depends entirely on the end-goal of the experiments and the locus to be modified. Investigators need to decide on the best nuclease to use for each experiment from among Zinc-Finger Nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 that would result in the highest likelihood of success with the fewest pitfalls. Furthermore, there have been significant improvements over the first-generation nucleases, such as the development of the dimeric CRISPR RNA-guided Fok1 nucleases (RFNs, marketed as NextGEN™ CRISPR) that reduces the “off-target” mutation rate, providing further options for investigators. Should researchers need to perform a point mutation, then considerations must be made between using single-stranded oligo-deoxynucleotides (ssODN) as the donor for homology-directed repair or utilizing a selection cassette within a donor vector in combination with an excision-only piggyBac™ transposase to leave a seamless edit. In this review, we will provide a general overview of the current technologies, along with methodologies for generating point mutations, while considering both their pros and cons.","PeriodicalId":91343,"journal":{"name":"Journal of stem cell and regenerative biology","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86114109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11