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Genetic Engineering to Induce Fetal-Like Hematopoietic Stem Cells 基因工程诱导胎儿样造血干细胞
Gene technology Pub Date : 2020-08-23 DOI: 10.35248/2329-6682.20.9.154
Bariş Ulum, S. Muljo
{"title":"Genetic Engineering to Induce Fetal-Like Hematopoietic Stem Cells","authors":"Bariş Ulum, S. Muljo","doi":"10.35248/2329-6682.20.9.154","DOIUrl":"https://doi.org/10.35248/2329-6682.20.9.154","url":null,"abstract":"Bone marrow transplantation (BMT) or hematopoietic stem cell transplantation (HSCT) is an archetype of cellular therapy. However, to date BMT still suffers from several complications. Recent technological advances have encouraged us to think about an alternative to traditional BMT. Specifically, we propose in utero HSCT (IUHSCT). For this purpose, we suggest that induced fetal-like hematopoietic stem cells (ifHSCs) might be suitable for IUHSCT, and should be seriously evaluated.","PeriodicalId":90831,"journal":{"name":"Gene technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42303651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Strategies and Best Practice in Cloning Small RNAs 小RNA克隆的策略和最佳实践
Gene technology Pub Date : 2020-08-03 DOI: 10.35248/2329-6682.20.9.151
Hui Dai, Weifeng Gu
{"title":"Strategies and Best Practice in Cloning Small RNAs","authors":"Hui Dai, Weifeng Gu","doi":"10.35248/2329-6682.20.9.151","DOIUrl":"https://doi.org/10.35248/2329-6682.20.9.151","url":null,"abstract":"High-throughput sequencing has become a standard and powerful tool for analyzing nucleic acids primarily due to its sensitivity and convenience. Small RNAs play important roles in regulating cellular and viral genes. The conventional methods for small RNA analyses are tedious and often lack accuracy, specificity and sensitivity for many small RNA species. Therefore, high-throughput sequencing becomes an indispensable tool for analyzing small RNAs. However, it is challenging to generate a reliable and representative small RNA library for high-throughput sequencing since small RNAs are usually expressed at extremely low levels and often contain modifications which affect library construction, usually causing biased readouts. This review compares various strategies for generating small RNA libraries of high quality and reliability, and provides recommendations on best practice in preparing high-throughput sequencing RNA libraries.","PeriodicalId":90831,"journal":{"name":"Gene technology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41911187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Strategies and Best Practice in Cloning Small RNAs. 克隆小rna的策略和最佳实践。
Gene technology Pub Date : 2020-08-03
Hui Dai, Weifeng Gu
{"title":"Strategies and Best Practice in Cloning Small RNAs.","authors":"Hui Dai,&nbsp;Weifeng Gu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>High-throughput sequencing has become a standard and powerful tool for analyzing nucleic acids primarily due to its sensitivity and convenience. Small RNAs play important roles in regulating cellular and viral genes. The conventional methods for small RNA analyses are tedious and often lack accuracy, specificity and sensitivity for many small RNA species. Therefore, high-throughput sequencing becomes an indispensable tool for analyzing small RNAs. However, it is challenging to generate a reliable and representative small RNA library for high-throughput sequencing since small RNAs are usually expressed at extremely low levels and often contain modifications which affect library construction, usually causing biased readouts. This review compares various strategies for generating small RNA libraries of high quality and reliability, and provides recommendations on best practice in preparing high-throughput sequencing RNA libraries.</p>","PeriodicalId":90831,"journal":{"name":"Gene technology","volume":"9 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38400944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic Engineering to Induce Fetal-Like Hematopoietic Stem Cells. 基因工程诱导胎儿样造血干细胞。
Gene technology Pub Date : 2020-01-01
Bariş Ulum, Stefan A Muljo
{"title":"Genetic Engineering to Induce Fetal-Like Hematopoietic Stem Cells.","authors":"Bariş Ulum,&nbsp;Stefan A Muljo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bone marrow transplantation (BMT) or hematopoietic stem cell transplantation (HSCT) is an archetype of cellular therapy. However, to date BMT still suffers from several complications. Recent technological advances have encouraged us to think about an alternative to traditional BMT. Specifically, we propose in utero HSCT (IUHSCT). For this purpose, we suggest that induced fetal-like hematopoietic stem cells (ifHSCs) might be suitable for IUHSCT, and should be seriously evaluated.</p>","PeriodicalId":90831,"journal":{"name":"Gene technology","volume":"9 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38360646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chromatin Memory in the Development of Human Cancers 染色质记忆在人类癌症发展中的作用
Gene technology Pub Date : 2014-08-11 DOI: 10.4172/2329-6682.1000114
Yixin Yao, Thomas L. Des Marais, Max Costa
{"title":"Chromatin Memory in the Development of Human Cancers","authors":"Yixin Yao, Thomas L. Des Marais, Max Costa","doi":"10.4172/2329-6682.1000114","DOIUrl":"https://doi.org/10.4172/2329-6682.1000114","url":null,"abstract":"Cancer is a complex disease with acquired genomic and epigenomic alterations that affect cell proliferation, viability and invasiveness. Almost all the epigenetic mechanisms including cytosine methylation and hydroxymethylation, chromatin remodeling and non-coding RNAs have been found associate with carcinogenesis and cancer specific expression profile. Altered histone modification as an epigenetic hallmark is frequently found in tumors. Understanding the epigenetic alterations induced by carcinogens or infectious agents may help us understand early epigenetic changes prior to the development of cancer. In this review, we focus on chromatin remodeling and the associated histone modifiers in the development of cancer; the application of these modifiers as a cancer therapy target in different clinical trial phases is also discussed.","PeriodicalId":90831,"journal":{"name":"Gene technology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70260788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Chromatin Memory in the Development of Human Cancers. 染色质记忆在人类癌症发展中的作用。
Gene technology Pub Date : 2014-08-11
Yixin Yao, Thomas L Des Marais, Max Costa
{"title":"Chromatin Memory in the Development of Human Cancers.","authors":"Yixin Yao,&nbsp;Thomas L Des Marais,&nbsp;Max Costa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cancer is a complex disease with acquired genomic and epigenomic alterations that affect cell proliferation, viability and invasiveness. Almost all the epigenetic mechanisms including cytosine methylation and hydroxymethylation, chromatin remodeling and non-coding RNAs have been found associate with carcinogenesis and cancer specific expression profile. Altered histone modification as an epigenetic hallmark is frequently found in tumors. Understanding the epigenetic alterations induced by carcinogens or infectious agents may help us understand early epigenetic changes prior to the development of cancer. In this review, we focus on chromatin remodeling and the associated histone modifiers in the development of cancer; the application of these modifiers as a cancer therapy target in different clinical trial phases is also discussed.</p>","PeriodicalId":90831,"journal":{"name":"Gene technology","volume":"3 2","pages":"114"},"PeriodicalIF":0.0,"publicationDate":"2014-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32990866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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