Metagenomics (Cairo, Egypt)最新文献

筛选
英文 中文
Metagenomics: Methods and Protocols 宏基因组学:方法和方案
Metagenomics (Cairo, Egypt) Pub Date : 2023-01-01 DOI: 10.1007/978-1-0716-2795-2
W. Streit, R. Daniel
{"title":"Metagenomics: Methods and Protocols","authors":"W. Streit, R. Daniel","doi":"10.1007/978-1-0716-2795-2","DOIUrl":"https://doi.org/10.1007/978-1-0716-2795-2","url":null,"abstract":"","PeriodicalId":90016,"journal":{"name":"Metagenomics (Cairo, Egypt)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90558630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
A microbial profiling method for the human microbiota using high-throughput sequencing. 一种利用高通量测序进行人类微生物群分析的方法。
Metagenomics (Cairo, Egypt) Pub Date : 2013-01-01 DOI: 10.4303/mg/235646
Huei-Hun Elizabeth Tseng, Meredith A J Hullar, Fei Li, Johanna W Lampe, Richard Sandstrom, Audra K Johnson, Lisa L Strate, Walter L Ruzzo, John Stamatoyannopoulos
{"title":"A microbial profiling method for the human microbiota using high-throughput sequencing.","authors":"Huei-Hun Elizabeth Tseng,&nbsp;Meredith A J Hullar,&nbsp;Fei Li,&nbsp;Johanna W Lampe,&nbsp;Richard Sandstrom,&nbsp;Audra K Johnson,&nbsp;Lisa L Strate,&nbsp;Walter L Ruzzo,&nbsp;John Stamatoyannopoulos","doi":"10.4303/mg/235646","DOIUrl":"https://doi.org/10.4303/mg/235646","url":null,"abstract":"<p><p>Study of the human microbiota in relation to human health and disease is a rapidly expanding field. To fully understand the complex relationship between the human gut microbiota and disease risks, study designs that capture the variation within and between human subjects at the population level are required, but this has been hampered by the lack of cost-effective methods to characterize this variation. Illumina sequencing is inexpensive and produces millions of reads per run, but it is unclear whether short reads can adequately represent the microbial community of a human host. In this study, we examined the utility of a profiling method, microbial nucleotide signatures (MNS), focused on low-depth sampling of the human microbiota using Ilumina short reads. This method is intended to aid in human population-based studies where large sample sizes are required to adequately capture variation in disease or phenotype differences. We found that, by calculating the nucleotide diversities along the sequenced 16S rRNA gene region, which did not require assembly or phylogenetic identification, we were able to differentiate the gut microbial nucleotide signatures of 9 healthy individuals. When we further subsampled the reads down to 40,000 reads (51 bp long) per sample, the diversity profiles were relatively unchanged. Applying MNS to a public datasets showed that it could differentiate body site differences. The scalability of our approach offers rapid classification of study participants for studies with the sample sizes required for epidemiological studies. Using MNS to classify the microbiome associated with a disease state followed by targeted in-depth sequencing will give a comprehensive understanding of the role of the microbiome in human health.</p>","PeriodicalId":90016,"journal":{"name":"Metagenomics (Cairo, Egypt)","volume":"2 ","pages":"235646"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764493/pdf/nihms477594.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31715069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信