International drug discovery最新文献

筛选
英文 中文
High Throughput, Label-free Screening Small Molecule Compound Libraries for Protein-Ligands using Combination of Small Molecule Microarrays and a Special Ellipsometry-based Optical Scanner. 利用小分子芯片和基于椭偏仪的特殊光学扫描仪,高通量、无标记地筛选蛋白质配体小分子化合物库。
International drug discovery Pub Date : 2011-12-01
James P Landry, Yiyan Fei, X D Zhu
{"title":"High Throughput, Label-free Screening Small Molecule Compound Libraries for Protein-Ligands using Combination of Small Molecule Microarrays and a Special Ellipsometry-based Optical Scanner.","authors":"James P Landry, Yiyan Fei, X D Zhu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Small-molecule compounds remain the major source of therapeutic and preventative drugs. Developing new drugs against a protein target often requires screening large collections of compounds with diverse structures for ligands or ligand fragments that exhibit sufficiently affinity and desirable inhibition effect on the target before further optimization and development. Since the number of small molecule compounds is large, high-throughput screening (HTS) methods are needed. Small-molecule microarrays (SMM) on a solid support in combination with a suitable binding assay form a viable HTS platform. We demonstrate that by combining an oblique-incidence reflectivity difference optical scanner with SMM we can screen 10,000 small-molecule compounds on a single glass slide for protein ligands without fluorescence labeling. Furthermore using such a label-free assay platform we can simultaneously acquire binding curves of a solution-phase protein to over 10,000 immobilized compounds, thus enabling full characterization of protein-ligand interactions over a wide range of affinity constants.</p>","PeriodicalId":89566,"journal":{"name":"International drug discovery","volume":" ","pages":"8-13"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3271728/pdf/nihms350115.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30437253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnosing and Treating Nervous System Disorders by Targeting Novel Classes of Non-coding RNAs. 通过靶向新型非编码 RNA 诊断和治疗神经系统疾病。
International drug discovery Pub Date : 2011-06-01
Irfan A Qureshi, Mark F Mehler
{"title":"Diagnosing and Treating Nervous System Disorders by Targeting Novel Classes of Non-coding RNAs.","authors":"Irfan A Qureshi, Mark F Mehler","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":89566,"journal":{"name":"International drug discovery","volume":"6 3","pages":"30-36"},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4675140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140195243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Implications of miRNAs in Colorectal Cancer Chemoresistance. mirna在结直肠癌化疗耐药中的意义。
International drug discovery Pub Date : 2011-02-01
Jingfang Ju
{"title":"Implications of miRNAs in Colorectal Cancer Chemoresistance.","authors":"Jingfang Ju","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>With the exponential growth of research efforts on non-coding microRNAs (miRNAs) in the past decade, miRNAs have been demonstrated to be important in many major human diseases, including diabetes, heart disease, and cancer. Due to the broad regulatory function of miRNAs, alterations of their expression can have profound consequences on multiple critical genes and pathways. One of the major issues related to the success of treating advanced colorectal cancer is chemoresistance. In this review, we will present some of the recent advancements in miRNA research related to chemoresistance mechanisms to 5-FU based chemotherapy in colorectal cancer and cancer stem cells. We believe that this miRNA-mediated resistance mechanism will offer novel strategies to develop future anti-cancer therapies.</p>","PeriodicalId":89566,"journal":{"name":"International drug discovery","volume":"2011 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350373/pdf/nihms299115.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33113194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信
小红书