IEEE/NIH Life Science Systems and Applications Workshop. IEEE/NIH Life Science Systems and Applications Workshop最新文献

An Image Driven Systems Biology Approach for Neurodegenerative Disease Studies in the TSC-mTOR Pathway. 神经退行性疾病研究中TSC-mTOR通路的图像驱动系统生物学方法。

IEEE/NIH Life Science Systems and Applications Workshop. IEEE/NIH Life Science Systems and Applications Workshop Pub Date : 2009-05-02 DOI: 10.1109/LISSA.2009.4906703

Dominik Beck, Xiaobo Zhou, Tuan Pham, Bernardo Sabatini, Stephen T C Wong

引用次数: 0

Fabrication of Polyvalent Therapeutic RNA Nanoparticles for Specific Delivery of siRNA, Ribozyme and Drugs to Targeted Cells for Cancer Therapy. 制备多价治疗性RNA纳米颗粒，将siRNA、核酶和药物特异性递送至癌症治疗的靶细胞。

IEEE/NIH Life Science Systems and Applications Workshop. IEEE/NIH Life Science Systems and Applications Workshop Pub Date : 2009-05-02 DOI: 10.1109/LISSA.2009.4906696

Yi Shu, Dan Shu, Zhijuan Diao, Guanxin Shen, Peixuan Guo

{"title":"Fabrication of Polyvalent Therapeutic RNA Nanoparticles for Specific Delivery of siRNA, Ribozyme and Drugs to Targeted Cells for Cancer Therapy.","authors":"Yi Shu, Dan Shu, Zhijuan Diao, Guanxin Shen, Peixuan Guo","doi":"10.1109/LISSA.2009.4906696","DOIUrl":"10.1109/LISSA.2009.4906696","url":null,"abstract":"Bacteriophage phi29 DNA packaging motor is geared by a six-pRNA ring. pRNA is able to form a multimeric complex and patterned superstructures via the interaction of two reengineered interlocking loops. This unique feature makes it an ideal polyvalent vehicle for nanomachine fabrication, pathogen detection, and the delivery of therapeutics. This report describes novel approaches for the fabrication of polyvalent therapeutic pRNA nanoparticles, especially tetramers for specific siRNA delivery to cancer cells and for the silencing of targeted genes. RNA 3-D design, circular permutation, folding energy alteration, and nucleotide modification were applied to generate stable RNA nanoparticles with low toxicity. Animal trials demonstrated the high efficiency of the polyvalent RNA nanoparticles in the prevention and treatment of cancer. Using such protein-free nanoparticles as therapeutic reagents would allow for long-term administration to avoid the induction of antibody due to repeated treatment for chronic diseases.","PeriodicalId":88894,"journal":{"name":"IEEE/NIH Life Science Systems and Applications Workshop. IEEE/NIH Life Science Systems and Applications Workshop","volume":"2009 ","pages":"9-12"},"PeriodicalIF":0.0,"publicationDate":"2009-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3019567/pdf/nihms182992.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29605110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Approaches for stoichiometry and distance determination of nanometer bio-complex by dual-channel single molecule imaging. 纳米生物复合物化学计量学及距离测定的双通道单分子成像方法。

IEEE/NIH Life Science Systems and Applications Workshop. IEEE/NIH Life Science Systems and Applications Workshop Pub Date : 2009-04-09 DOI: 10.1109/LISSA.2009.4906725

Hui Zhang, Dan Shu, Mark Browne, Peixuan Guo

引用次数: 0

An Image Informatics Method for Automated Quantitative Analysis of Phenotype Visual Similarities. 一种用于表型视觉相似性自动定量分析的图像信息学方法。

IEEE/NIH Life Science Systems and Applications Workshop. IEEE/NIH Life Science Systems and Applications Workshop Pub Date : 2009-04-09 DOI: 10.1109/LISSA.2009.4906718

Lior Shamir, D Mark Eckley, John Delaney, Nikita Orlov, Ilya G Goldberg

引用次数: 0

Identifying Components in 3D Density Maps of Protein Nanomachines by Multi-scale Segmentation. 通过多尺度分割识别蛋白质纳米机器三维密度图中的组成部分

IEEE/NIH Life Science Systems and Applications Workshop. IEEE/NIH Life Science Systems and Applications Workshop Pub Date : 2009-04-01 DOI: 10.1109/LISSA.2009.4906705

Grigore Pintilie, Junjie Zhang, Wah Chiu, David Gossard

{"title":"Identifying Components in 3D Density Maps of Protein Nanomachines by Multi-scale Segmentation.","authors":"Grigore Pintilie, Junjie Zhang, Wah Chiu, David Gossard","doi":"10.1109/LISSA.2009.4906705","DOIUrl":"10.1109/LISSA.2009.4906705","url":null,"abstract":"Segmentation of density maps obtained using cryo-electron microscopy (cryo-EM) is a challenging task, and is typically accomplished by time-intensive interactive methods. The goal of segmentation is to identify the regions inside the density map that correspond to individual components. We present a multi-scale segmentation method for accomplishing this task that requires very little user interaction. The method uses the concept of scale space, which is created by convolution of the input density map with a Gaussian filter. The latter process smoothes the density map. The standard deviation of the Gaussian filter is varied, with smaller values corresponding to finer scales and larger values to coarser scales. Each of the maps at different scales is segmented using the watershed method, which is very efficient, completely automatic, and does not require the specification of seed points. Some detail is lost in the smoothing process. A sharpening process reintroduces detail into the segmentation at the coarsest scale by using the segmentations at the finer scales. We apply the method to simulated density maps, where the exact segmentation (or ground truth) is known, and rigorously evaluate the accuracy of the resulting segmentations.","PeriodicalId":88894,"journal":{"name":"IEEE/NIH Life Science Systems and Applications Workshop. IEEE/NIH Life Science Systems and Applications Workshop","volume":"2009 ","pages":"44-47"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885738/pdf/nihms134981.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29059654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Image Based System Biology Approach for Alzheimer's Disease Pathway Analysis. 基于图像的阿尔茨海默病通路分析系统生物学方法。

IEEE/NIH Life Science Systems and Applications Workshop. IEEE/NIH Life Science Systems and Applications Workshop Pub Date : 2009-04-01 DOI: 10.1109/lissa.2009.4906726

Yue Huang, Xiaobo Zhou, Benchun Miao, Marta Lipinski, Zheng Xia, Guangshu Hu, Alexei Degterev, Junying Yuan, Stephen T C Wong

引用次数: 0