Glycobiology insights最新文献

Glycosaminoglycans in Human and Bovine Serum: Detection of Twenty-Four Heparan Sulfate and Chondroitin Sulfate Motifs Including a Novel Sialic Acid-modified Chondroitin Sulfate Linkage Hexasaccharide. 人血清和牛血清中的糖胺聚糖：24个硫酸肝素和硫酸软骨素基基的检测，包括一种新型唾液酸修饰的硫酸软骨素键六糖。

Glycobiology insights Pub Date : 2010-02-09

Hong Lu, Lynda M McDowell, Daniel R Studelska, Lijuan Zhang

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引用次数: 0

Glycosaminoglycans in Human and Bovine Serum: Detection of Twenty-Four Heparan Sulfate and Chondroitin Sulfate Motifs Including a Novel Sialic Acid-modified Chondroitin Sulfate Linkage Hexasaccharide. 人血清和牛血清中的糖胺聚糖:24个硫酸肝素和硫酸软骨素基基的检测，包括一种新型唾液酸修饰的硫酸软骨素键六糖。

Glycobiology insights Pub Date : 2010-02-09 DOI: 10.4137/GBI.S4273

Hong Lu, L. McDowell, D. Studelska, Lijuan Zhang

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引用次数: 44

Identification of Chemically Sulfated/desulfated Glycosaminoglycans in Contaminated Heparins and Development of a Simple Assay for the Detection of Most Contaminants in Heparin. 污染肝素中化学磺化/去硫化糖胺聚糖的鉴定及肝素中大多数污染物的简易检测方法的建立。

Glycobiology insights Pub Date : 2010-02-02

Jing Pan, Yi Qian, Xiaodong Zhou, Andrew Pazandak, Sarah B Frazier, Peter Weiser, Hong Lu, Lijuan Zhang

{"title":"Identification of Chemically Sulfated/desulfated Glycosaminoglycans in Contaminated Heparins and Development of a Simple Assay for the Detection of Most Contaminants in Heparin.","authors":"Jing Pan, Yi Qian, Xiaodong Zhou, Andrew Pazandak, Sarah B Frazier, Peter Weiser, Hong Lu, Lijuan Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Contaminated heparin was linked to at least 149 deaths and hundreds of adverse reactions. Published report indicates that heparin contaminants were a natural impurity, dermatan sulfate, and a contaminant, oversulfated chondroitin sulfate (OSCS). OSCS was assumed to derive from animal cartilage. By analyzing 26 contaminated heparin lots from different sources, our data indicate that the heparin contaminants were chemically sulfated or chemically sulfated/desulfated glycosaminoglycans (GAGs) consisting of heparan sulfate, chondroitin sulfate, and dermatan sulfate based on monosaccharide quantification, CE, heparin lyase digestion, and liquid chromatography/mass spectrometry analysis. Since currently recommended heparin quality control assays had failed to detect certain heparin contaminants, a simple method that detects most contaminants in heparin was developed. This assay detects specific heparin structures that most contaminants cannot mimic and can be performed in any laboratory equipped with an UV spectrometer.","PeriodicalId":88877,"journal":{"name":"Glycobiology insights","volume":"2010 2","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2010-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2909132/pdf/nihms178983.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29146358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Chemically Sulfated/desulfated Glycosaminoglycans in Contaminated Heparins and Development of a Simple Assay for the Detection of Most Contaminants in Heparin. 污染肝素中化学磺化/去硫化糖胺聚糖的鉴定及肝素中大多数污染物的简易检测方法的建立。

Glycobiology insights Pub Date : 2010-02-02 DOI: 10.4137/GBI.S4237

Jing Pan, Y. Qian, Xiao-yan Zhou, Andrew Pazandak, Sarah B Frazier, P. Weiser, Hong Lu, Lijuan Zhang

{"title":"Identification of Chemically Sulfated/desulfated Glycosaminoglycans in Contaminated Heparins and Development of a Simple Assay for the Detection of Most Contaminants in Heparin.","authors":"Jing Pan, Y. Qian, Xiao-yan Zhou, Andrew Pazandak, Sarah B Frazier, P. Weiser, Hong Lu, Lijuan Zhang","doi":"10.4137/GBI.S4237","DOIUrl":"https://doi.org/10.4137/GBI.S4237","url":null,"abstract":"Contaminated heparin was linked to at least 149 deaths and hundreds of adverse reactions. Published report indicates that heparin contaminants were a natural impurity, dermatan sulfate, and a contaminant, oversulfated chondroitin sulfate (OSCS). OSCS was assumed to derive from animal cartilage. By analyzing 26 contaminated heparin lots from different sources, our data indicate that the heparin contaminants were chemically sulfated or chemically sulfated/desulfated glycosaminoglycans (GAGs) consisting of heparan sulfate, chondroitin sulfate, and dermatan sulfate based on monosaccharide quantification, CE, heparin lyase digestion, and liquid chromatography/mass spectrometry analysis. Since currently recommended heparin quality control assays had failed to detect certain heparin contaminants, a simple method that detects most contaminants in heparin was developed. This assay detects specific heparin structures that most contaminants cannot mimic and can be performed in any laboratory equipped with an UV spectrometer.","PeriodicalId":88877,"journal":{"name":"Glycobiology insights","volume":"2010 2 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2010-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70701413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18