Biochemistry eJournal最新文献

Possibility of teaching Bacillus subtilis ethylene glycol utilization 传授枯草芽孢杆菌乙二醇利用的可能性

Biochemistry eJournal Pub Date : 2021-03-29 DOI: 10.2139/ssrn.3814481

Wenfa Ng

引用次数: 0

The Study of Carboxylic Acids in Flowers and Leaves of Veronica Chamaedrys L. and Veronica Teucrium L. 山茱萸和山茱萸花和叶中羧酸的研究。

Biochemistry eJournal Pub Date : 2020-12-30 DOI: 10.15587/2519-4852.2020.221078

А. Kovaleva, T. Ilina, A. Raal, A. Osmachko, O. Goryacha, Y. Rozhkovskyi

{"title":"The Study of Carboxylic Acids in Flowers and Leaves of Veronica Chamaedrys L. and Veronica Teucrium L.","authors":"А. Kovaleva, T. Ilina, A. Raal, A. Osmachko, O. Goryacha, Y. Rozhkovskyi","doi":"10.15587/2519-4852.2020.221078","DOIUrl":"https://doi.org/10.15587/2519-4852.2020.221078","url":null,"abstract":"The aim. The genus Veronica (Veronica L.), family Plantaginaceae Juss. in the flora of Ukraine is represented by 64 species; Veronica chamaedrys L. and Veronica teucrium L. are widespread in the Kharkiv region. Plants are used in folk medicine as expectorants, anti-inflammatory, diaphoretic, anti-allergic, choleretic, antispasmodic, anticonvulsant, diuretic, sedative, wound healing and antibacterial agents. The aim of this work was to study the carboxylic acids of flowers and leaves of Veronica chamaedrys L. and flowers and leaves of Veronica teucrium L. Materials and methods. The objects of the study were flowers and leaves of Veronica chamaedrys L. and Veronica teucrium L., harvested in the flowering phase in 2018 in Kharkiv region, Ukraine. The study of carboxylic acids was performed by chromatography-mass spectrometry on a 6890N MSD/DS Agilent Technologies chromatograph with a 5973N mass spectrometric detector. Identification of acid methyl esters was performed using data from the mass spectrum library NIST 05 and Willey 2007 in combination with programs for the identification of AMDIS and NIST; also compared the retention time with the retention time of standard compounds. Results. In the flowers and leaves of Veronica teucrium L. 35 carboxylic acids were identified, the total content of which is 5.55 % and 2.93 %, respectively. 31 and 32 carboxylic acids were identified in the flowers and leaves of Veronica chamaedrys L., their total content is 5.39 % and 7.45 %, respectively. Conclusions. It is established that the flowers and leaves of Veronica chamaedrys L. are characterized by a higher content of carboxylic acids compared to the flowers and leaves of Veronica teucrium L. As chemotaxonomic markers of flowers and leaves the following compounds can be used: α-furanic acid for Veronica chamaedrys L.; veratric, 4-hydroxybenzylacetic and syringic acids for Veronica teucrium L. The obtained results will be the basis for further chemotaxonomic studies","PeriodicalId":8795,"journal":{"name":"Biochemistry eJournal","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78142006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interstellar Formation of Biorelevant Pyruvic Acid (CH 3COCOOH) 生物相关丙酮酸(ch3cooh)的星际形成

Biochemistry eJournal Pub Date : 2020-08-12 DOI: 10.2139/ssrn.3652328

N. Kleimeier, A. Eckhardt, P. Schreiner, R. Kaiser

引用次数: 19

The Transmission and Toxicity of Phpma Copolymer-Bound Doxorubicin Containing Exosomes Derived from Adherent Two-Dimensional Human Breast Adenocarcinoma Cell Line and Three-Dimensional Spheroids 粘附二维人乳腺腺癌细胞系和三维球体衍生的Phpma共聚物结合含阿霉素的外泌体的传播和毒性

Biochemistry eJournal Pub Date : 2020-01-01 DOI: 10.2139/ssrn.3692012

Kristýna Gunár, L. Kotrchová, M. Filipová, Tereza Krunclová, R. Pola, E. Randárová, T. Etrych, O. Janoušková

{"title":"The Transmission and Toxicity of Phpma Copolymer-Bound Doxorubicin Containing Exosomes Derived from Adherent Two-Dimensional Human Breast Adenocarcinoma Cell Line and Three-Dimensional Spheroids","authors":"Kristýna Gunár, L. Kotrchová, M. Filipová, Tereza Krunclová, R. Pola, E. Randárová, T. Etrych, O. Janoušková","doi":"10.2139/ssrn.3692012","DOIUrl":"https://doi.org/10.2139/ssrn.3692012","url":null,"abstract":"Exosomes are endosomally-derived vesicles. Their composition is significantly affected by physiological state of the donor cells and can reflect changes in the cell microenvironment as well as of the whole body. Exosomes can manipulate local and systemic environment to influence cancer progression and dissemination and also modulate immune response. Moreover, exosomes have been investigated as possible transmission nanocarriers for therapeutics. In this study, exosome loading was achieved by incubation of free doxorubicin (DOX) and DOX bound to N -(2-hydroxypropyl) methacrylamide (HPMA)-based copolymers (pHPMA) with an adherent human breast adenocarcinoma cell line and its derived spheroids. Both free and p(HPMA)-bound DOX were successfully loaded into exosomes with different loading efficiencies. Spheroids secreted significantly more exosomes than adherent cells. These exosomes differed in abundance of their exosomal markers. The adherent cell line showed a decreased viability after treatment with free DOX or pHPMA bound-DOX-loaded exosomes, confirming the successful transmission of both, free cancerostatics and polymer bound cancerostatics by exosomes. To our knowledge, this is the first proof of pHPMA-drug conjugates secretion by extracellular vesicles, providing a new perspective on the transmission of drug/polymeric drugs in tumor tissue via exosomes. Interestingly, results obtained within the manuscript contribute to the explanation of high therapeutic activity of pH-sensitive polymer-DOX conjugates, which we suppose, is based on the exosome-based cell-to-cell delivery within the tumor tissue after the passive accumulation of the polymer-drug system.","PeriodicalId":8795,"journal":{"name":"Biochemistry eJournal","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83790103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Polymerization-Driven Hierarchical Co-Assembly of Micelles for Access to Mesoporous Hollow Metal Coordination Bio-Polymers 介孔中空金属配位生物聚合物中胶束的聚合驱动分层共组装

Biochemistry eJournal Pub Date : 2019-09-04 DOI: 10.2139/ssrn.3448140

Yan Ai, Yuxiou You, Facai Wei, Xiaolin Jiang, Zhuolei Han, Jing Cui, Hao Luo, Yucen Li, Zhixin Xu, Jun Yang, Q. Bao, C. Jing, Jianwei Fu, Jiangong Cheng, Shaohua Liu

{"title":"Polymerization-Driven Hierarchical Co-Assembly of Micelles for Access to Mesoporous Hollow Metal Coordination Bio-Polymers","authors":"Yan Ai, Yuxiou You, Facai Wei, Xiaolin Jiang, Zhuolei Han, Jing Cui, Hao Luo, Yucen Li, Zhixin Xu, Jun Yang, Q. Bao, C. Jing, Jianwei Fu, Jiangong Cheng, Shaohua Liu","doi":"10.2139/ssrn.3448140","DOIUrl":"https://doi.org/10.2139/ssrn.3448140","url":null,"abstract":"Bio-inspired hierarchical self-assembly provides elegant and powerful bottom-up strategies for the creation of complex materials. However, the current self-assembly approaches for natural bio-compounds often result in materials with limited diversity and complexity in architecture as well as microstructure. Here, we develop a novel coordination polymerization-driven hierarchical assembly of micelle strategy, using phytic acid-based natural compounds as an example, for the spatially controlled fabrication of metal coordination bio-polymers. The aliphatic surfactant pentadecafluorooctanoic acid and block co-polymer of polystyrene-b-poly (ethylene oxide) are assembled into two kinds of the discrete amphiphilic micelles. They evolve into hollow vesicles surrounding with spherical micelles, driven by the strong interaction triggered by coordination polymerization of ferric phytate precursors, which in turn facilitate the growth of precursors in a confined space. Consequently, the synchronous control on morphology and mesoscale structure for bio-compounds was successfully achieved for the first time. The resultant ferric phytate bio-polymer nanospheres feature hollow architecture, ordered meso-channels of ~12 nm, high surface area of 401 m2g-1, and large pore volume of 0.53 cm3g-1. As an advanced anode material, this bio-derivative metal coordination polymer delivers a remarkable reversible capacity of 540 mAh g-1 at 50 mA g-1, good rate capability and cycling stability for the use in sodium ion batteries. This study holds great potential of the design of new complex bio-materials with supramolecular chemistry.","PeriodicalId":8795,"journal":{"name":"Biochemistry eJournal","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84644608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Unique Methionine-Rich Protein - Aragonite Crystal Complex: Structure and Mechanical Functions of the Pinctada Fucata Bivalve Hinge Ligament 一种独特的富含蛋氨酸的蛋白质-文石晶体复合物:富喀塔双壳合叶韧带的结构和力学功能

Biochemistry eJournal Pub Date : 2019-07-23 DOI: 10.2139/ssrn.3424545

Michio Suzuki, K. Kubota, R. Nishimura, L. Negishi, K. Komatsu, H. Kagi, Katya Rehav, S. Cohen, I. Pinkas

{"title":"A Unique Methionine-Rich Protein - Aragonite Crystal Complex: Structure and Mechanical Functions of the Pinctada Fucata Bivalve Hinge Ligament","authors":"Michio Suzuki, K. Kubota, R. Nishimura, L. Negishi, K. Komatsu, H. Kagi, Katya Rehav, S. Cohen, I. Pinkas","doi":"10.2139/ssrn.3424545","DOIUrl":"https://doi.org/10.2139/ssrn.3424545","url":null,"abstract":"The bivalve hinge ligament holds the two shells together. The ligament functions as a spring to open the shells after they were closed by the adductor muscle. The ligament is a mineralized tissue that bears no resemblance to any other known tissue. About half the ligament is composed of a protein-rich matrix, and half of long and extremely thin segmented aragonite crystals. Here we study the hinge ligament of the pearl oyster Pinctada fucata. FIB SEM shows that the 3D organization is remarkably ordered. The full sequence of the major protein component contains a continuous segment of 30 repeats of MMMLPD. There is no known homologous protein. Knockdown of this protein prevents crystal formation, demonstrating that the integrity of the matrix is necessary for crystals to form. X-ray diffraction shows that the aragonite crystals are more aligned in the compressed ligament, indicating that the crystals may be actively contributing to the elastic properties. The fusion interphase that joins the ligament to the shell nacre is composed of a prismatic mineralized tissue with a thin organic-rich layer at its center. Nanoindentation of the dry interphase shows that the elastic modulus of the nacre adjacent to the interphase gradually decreases until it approximates that of the interphase. The interphase modulus slightly increases until it matches the ligament. All these observations demonstrate that the ligament shell complex is a remarkable biological tissue that has evolved unique properties that enable bivalves to open their shell effectively innumerable times during the lifetime of the animal.","PeriodicalId":8795,"journal":{"name":"Biochemistry eJournal","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77608993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Impact of Automated Guidance for Mechanical Control of Herbicide Resistant Weeds in Corn 自动导引对玉米抗除草剂杂草机械防治的影响

Biochemistry eJournal Pub Date : 2017-06-26 DOI: 10.2139/ssrn.2992647

T. Griffin, J. Lowenberg-Deboer

引用次数: 6