{"title":"Editorial | A New Path Forward for Forensic Science Learning","authors":"G. J. Laughlin","doi":"10.59082/ylbr4847","DOIUrl":"https://doi.org/10.59082/ylbr4847","url":null,"abstract":"Amazing but true, many criminalists regard forensic microscopy and the light microscope as the single most important instrument in their laboratories for the examination of physical trace evidence. They emphasize proper training as the key to success. McCrone Research Institute began by teaching chemical microscopy courses with the polarized light microscope to chemists and forensic scientists first at Cornell University, then Armour Research Foundation (ARF), Illinois Institute of Technology (IIT), University of Illinois at Chicago (UIC), and in its classrooms and laboratories still located on the South Side of Chicago since 1960. But it hasn’t been an easy path.","PeriodicalId":82002,"journal":{"name":"Microscope (Carshalton Beeches (Surrey))","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41419113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Calibration of Dispersion Staining Colors","authors":"S. Su","doi":"10.59082/hnqr9171","DOIUrl":"https://doi.org/10.59082/hnqr9171","url":null,"abstract":"Dispersion staining (DS) is an effective and versatile technique for measuring the refractive index (RI) of non-opaque materials by polarized light microscopy (PLM) and has been widely applied in the analysis of asbestos minerals. DS converts the RI difference between an object and its surrounding liquid medium with known RI into an observable color, i.e., the DS color, in the visible spectrum, resulting from the corresponding matching wavelength λm between the RI of the object and its surrounding liquid. Based on the relationship between DS color and λm, λm can be quantitatively evaluated from the observed DS color. Once λm is known, the RI difference between the object and liquid can be calculated. From the liquid’s RI value, the RI value of the object is then measured. The accuracy of RI measurement is directly and primarily dependent on the accuracy of the DS color estimation. Therefore, it’s paramount to obtain an accurate estimation of DS color. In order to improve the accuracy of DS color estimation, it must be carefully calibrated against the specific polarized light microscope used and the specific eyes of the analyst performing the measurement. This paper presents a practical, step-by-step procedure for the calibration of DS color with necessary RI-λm conversion tables at different working temperatures.","PeriodicalId":82002,"journal":{"name":"Microscope (Carshalton Beeches (Surrey))","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45764434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Critical Focus | Farewell Reality, Hello Jellybeans","authors":"B. Ford","doi":"10.59082/zvpr7482","DOIUrl":"https://doi.org/10.59082/zvpr7482","url":null,"abstract":"Social media are already subverting scientific truth, and our conventional view of the living cell is being replaced with mythical models that look more like blobs and jellybeans. The other afternoon I was doing what we all do, peering intently down the microscope and marveling at the unique spectacles we see, when suddenly the world went away. You remember the real world we knew -- a place with the solidity of science and the certainty of facts. If you weren't sure, you would consult the acknowledged authority who would soon put you right. Or you would look it up. Not anymore. Suddenly that world disappeared. There are no facts any longer, and authorities exist not because the scholarly world acknowledges their wisdom, but because they assure you that's what they are.","PeriodicalId":82002,"journal":{"name":"Microscope (Carshalton Beeches (Surrey))","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45629745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"What We See Part 1: Morphological Properties of Particles in a Fixed Mount","authors":"Russ Crutcher, Heidie Crutcher","doi":"10.59082/ceut7303","DOIUrl":"https://doi.org/10.59082/ceut7303","url":null,"abstract":"We often identify what we expect to see, what is familiar, or what we want to see based on limited information (1). For example, while reading we may skim a word based on the first and last letters; however, it may not be the actual word. The brain is very busy, and it often skips ahead when it comes to a conclusion based on a limited set of data. We may have to go back and view all the letters to identify the actual word. Looking at particles under the light microscope is similar. Working memory is limited at any given time and must be regularly updated while the many visible properties of a particle are examined. There are more than 40 optical properties that may be used to characterize a particle in a fixed mounting medium. Information on how the particle was generated, where it came from, how it was transported, its composition, and other aspects of its history are often written in its optical properties. A list of the morphological properties is presented here with information on how to look for them. Without such a list it is easy to overlook important characteristics that may be critical in understanding how a particle relates to the analytical questions being asked.","PeriodicalId":82002,"journal":{"name":"Microscope (Carshalton Beeches (Surrey))","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45577469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Sparenga, G. J. Laughlin, Meggan B. King, Dean Golemis
{"title":"New Microcrystal Tests for Controlled Drugs, Diverted Pharmaceuticals, and Bath Salts (Synthetic Cathinones): Alpha-PVP","authors":"S. Sparenga, G. J. Laughlin, Meggan B. King, Dean Golemis","doi":"10.59082/smcu8705","DOIUrl":"https://doi.org/10.59082/smcu8705","url":null,"abstract":"The Microscope is publishing selected monographs from McCrone Research Institute’s recently completed research, New Microcrystal Tests for Controlled Drugs, Diverted Pharmaceuticals, and Bath Salts (Synthetic Cathinones), which contains newly developed microcrystal tests and reagents with 9 additional drugs: alprazolam, butylone, mephedrone, methylone, MDPV, 4-MEC, alpha-PVP, tramadol, and zolpidem. This issue includes the monographs for the following drugs/reagents: - Alpha-PVP/palladium chloride with hydrochloric acid and phosphoric acid - Alpha-PVP/potassium ferrocyanide with hydrochloric acid","PeriodicalId":82002,"journal":{"name":"Microscope (Carshalton Beeches (Surrey))","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41407637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
David Berry, Jed Januch, Lynn Woodbury, Douglas Kent
{"title":"Detection of Erionite and Other Zeolite Fibers in Soil by the Fluidized Bed Preparation Methodology.","authors":"David Berry, Jed Januch, Lynn Woodbury, Douglas Kent","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Erionite is a zeolite mineral that can occur as fibrous particles in soil. Inhalation exposure to erionite fibers may result in increased risk of diseases, such as mesothelioma. Low level detection of mineral fibers in soils has traditionally been accomplished using polarized light microscopy (PLM) methods to analyze bulk samples providing detection limits of around 0.25% by weight. This detection level may not be sufficiently low enough for protection of human health and is subject to large variability between laboratories. The fluidized bed asbestos segregator (FBAS) soil preparation method uses air elutriation to separate mineral fibers, such as erionite, from soil particles with higher aerodynamic diameter and deposits those mineral fibers onto filters that can be quantitatively analyzed by microscopic techniques, such as transmission electron microscopy (TEM). In this study, performance evaluation (PE) standards of erionite in soil with nominal concentrations ranging from 0.1% to 0.0001% by weight were prepared using the FBAS soil preparation method and the resulting filters were analyzed by TEM. The analytical results of this study illustrate a linear relationship between the nominal concentration of erionite (as % by weight) in the PE standard and the concentration estimated by TEM analysis expressed as erionite structures per gram of test material (s/g). A method detection limit of 0.003% by weight was achieved, which is approximately 100 times lower than typical detection limits for soils by PLM. The FBAS soil preparation method was also used to evaluate authentic field soil samples to better estimate the concentrations of erionite in soils on a weight percent basis. This study demonstrates the FBAS preparation method, which has already been shown to reliably detect low levels of asbestos in soil, can also be used to quantify low levels of erionite in soil.</p>","PeriodicalId":82002,"journal":{"name":"Microscope (Carshalton Beeches (Surrey))","volume":"67 4","pages":"147-158"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376948/pdf/nihms-1598663.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38194370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An early microscope by John Benjamin Dancer of Manchester.","authors":"R H Nuttali","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":82002,"journal":{"name":"Microscope (Carshalton Beeches (Surrey))","volume":" ","pages":"93-100"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28536219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}