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Simultaneous Dual-region Functional Imaging in Miniaturized Two-photon Microscopy. 小型化双光子显微镜同时双区功能成像。
Biomedical optics (Washington, D.C.) Pub Date : 2024-04-01 DOI: 10.1364/brain.2024.bm3c.4
Zixiao Zhang, Shing-Jiuan Liu, Ben Mattison, Weijian Yang
{"title":"Simultaneous Dual-region Functional Imaging in Miniaturized Two-photon Microscopy.","authors":"Zixiao Zhang, Shing-Jiuan Liu, Ben Mattison, Weijian Yang","doi":"10.1364/brain.2024.bm3c.4","DOIUrl":"10.1364/brain.2024.bm3c.4","url":null,"abstract":"<p><p>We demonstrate simultaneous dual-region in-vivo imaging of brain activity in mouse cortex through a miniaturized spatial-multiplexed two-photon microscope platform, which doubles the imaging speed. Neuronal signals from the two regions are computationally demixed and extracted.</p>","PeriodicalId":72390,"journal":{"name":"Biomedical optics (Washington, D.C.)","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Time-Multiplexed Miniaturized Two-Photon Microscopy. 时间复用小型化双光子显微镜。
Biomedical optics (Washington, D.C.) Pub Date : 2024-04-01 DOI: 10.1364/brain.2024.bm3c.2
Shing-Jiuan Liu, Zixiao Zhang, Ben Mattison, Weijian Yang
{"title":"Time-Multiplexed Miniaturized Two-Photon Microscopy.","authors":"Shing-Jiuan Liu, Zixiao Zhang, Ben Mattison, Weijian Yang","doi":"10.1364/brain.2024.bm3c.2","DOIUrl":"10.1364/brain.2024.bm3c.2","url":null,"abstract":"<p><p>We propose a time-multiplexed miniaturized two-photon microscope (TM-MINI2P), enabling a two-fold increase in imaging speed while maintaining a high spatial resolution. Using TM-MINI2P, we conducted high-speed in-vivo calcium imaging in mouse cortex.</p>","PeriodicalId":72390,"journal":{"name":"Biomedical optics (Washington, D.C.)","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GigaFIBI; Rapid, Large-format Histology-resolution Imaging for Intraoperative Assessment of Breast Lumpectomy Margins. GigaFIBI;快速、大画幅组织分辨率成像术中评估乳腺肿瘤切除边缘。
Biomedical optics (Washington, D.C.) Pub Date : 2024-04-01 DOI: 10.1364/MICROSCOPY.2024.MS1A.2
Dena Sayrafi, Nate Anderson, Candice Sauder, Diana Miglioretti, Richard Levenson, Alexander Borowsky, Farzad Fereidouni
{"title":"GigaFIBI; Rapid, Large-format Histology-resolution Imaging for Intraoperative Assessment of Breast Lumpectomy Margins.","authors":"Dena Sayrafi, Nate Anderson, Candice Sauder, Diana Miglioretti, Richard Levenson, Alexander Borowsky, Farzad Fereidouni","doi":"10.1364/MICROSCOPY.2024.MS1A.2","DOIUrl":"https://doi.org/10.1364/MICROSCOPY.2024.MS1A.2","url":null,"abstract":"<p><p>GigaFIBI, an advancement of Fluorescence Imitating Brightfield Imaging (FIBI), displays significant promise in reducing positive margins and associated costs by capturing histology-grade images of 100×100 within minutes, offering intraoperative guidance.</p>","PeriodicalId":72390,"journal":{"name":"Biomedical optics (Washington, D.C.)","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12055199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioluminescence tomography system for in vivo irradiation guidance. 用于体内照射引导的生物发光断层成像系统。
Biomedical optics (Washington, D.C.) Pub Date : 2022-04-01 DOI: 10.1364/OTS.2022.OTu2D.3
Zijian Deng, Xiangkun Xu, Iulian Iordachita, Hamid Dehghani, Bin Zhang, John W Wong, Ken Kang-Hsin Wang
{"title":"Bioluminescence tomography system for in vivo irradiation guidance.","authors":"Zijian Deng,&nbsp;Xiangkun Xu,&nbsp;Iulian Iordachita,&nbsp;Hamid Dehghani,&nbsp;Bin Zhang,&nbsp;John W Wong,&nbsp;Ken Kang-Hsin Wang","doi":"10.1364/OTS.2022.OTu2D.3","DOIUrl":"https://doi.org/10.1364/OTS.2022.OTu2D.3","url":null,"abstract":"<p><p>We constructed a bioluminescence tomography(BLT) to localize soft tissue targets for preclinical radiotherapy study. With the threshold and margin designed for target volume, BLT can provide opportunity to perform conformal irradiation to malignancy.</p>","PeriodicalId":72390,"journal":{"name":"Biomedical optics (Washington, D.C.)","volume":"2022 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10047798/pdf/nihms-1884426.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9224158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultra-sensitive single pixel bioluminescence tomography for in vivo cell tracking. 用于体内细胞跟踪的超灵敏单像素生物发光断层扫描。
Biomedical optics (Washington, D.C.) Pub Date : 2022-04-01 DOI: 10.1364/translational.2022.jtu3a.45
Zhishen Tong, Zijian Deng, Ken Kang-Hsin Wang
{"title":"Ultra-sensitive single pixel bioluminescence tomography for <i>in vivo</i> cell tracking.","authors":"Zhishen Tong,&nbsp;Zijian Deng,&nbsp;Ken Kang-Hsin Wang","doi":"10.1364/translational.2022.jtu3a.45","DOIUrl":"https://doi.org/10.1364/translational.2022.jtu3a.45","url":null,"abstract":"<p><p>We presented a single-pixel bioluminescence tomography (SPBLT) to monitor single or few cells in live animal. Simulations are proposed to validate the capability of SPBLT in detecting weak bioluminescence signal emitted from cells <i>in vivo</i>.</p>","PeriodicalId":72390,"journal":{"name":"Biomedical optics (Washington, D.C.)","volume":"2022 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044448/pdf/nihms-1884425.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9224154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a Mobile Fluorescence Tomography-guided System for Pre-clinical Radiotherapy Research. 用于临床前放疗研究的移动荧光层析引导系统的研制。
Biomedical optics (Washington, D.C.) Pub Date : 2020-04-01 DOI: 10.1364/ots.2020.sw1d.6
Luke Hardy, Daniel Sforza, Iulian Iordachita, Xiangkun Xu, John W Wong, Ken Kang-Hsin Wang
{"title":"Development of a Mobile Fluorescence Tomography-guided System for Pre-clinical Radiotherapy Research.","authors":"Luke Hardy,&nbsp;Daniel Sforza,&nbsp;Iulian Iordachita,&nbsp;Xiangkun Xu,&nbsp;John W Wong,&nbsp;Ken Kang-Hsin Wang","doi":"10.1364/ots.2020.sw1d.6","DOIUrl":"https://doi.org/10.1364/ots.2020.sw1d.6","url":null,"abstract":"<p><p>We proposed to build a mobile fluorescence tomography (mFT) system as an image-guided platform for pre-clinical radiotherapy research. The mFT system is expected to localize functional target/tumor, guide irradiation, and provide longitudinal treatment assessment.</p>","PeriodicalId":72390,"journal":{"name":"Biomedical optics (Washington, D.C.)","volume":"2020 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457533/pdf/nihms-1644934.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39443733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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