中华胰腺病杂志最新文献

{"title":"Evaluation of BISAP and HAP scoring system in predicting prognosis of acute pancreatitis","authors":"Xin Li, K. Chao, J. Yao, B. Zhong, Minhu Chen","doi":"10.3760/CMA.J.ISSN.1674-1935.2013.03.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-1935.2013.03.004","url":null,"abstract":"Objective To evaluate the bedside index for severity in acute pancreatitis (BISAP) and harmless acute pancreatitis (HAP) scoring system in predicting prognosis of acute pancreatitis (AP).Methods A total of 442 AP patients,who were admitted to The First Affiliated Hospital of Sun Yat-sen University from January 2003 to December 2010,were retrospectively studied.BISAP and HAP scores were evaluated respectively.The value of BISAP and HAP scores in predicting severity,local complications,organ failure and mortality were measured by the area under the curve (AUC) of receiver operator characteristic curve (ROC),and it was compared with that of traditional Ranson's score.Results Among 442 patients,73 patients (16.5％) were diagnosed to have severe acute pancreatitis (SAP).AUC for BISAP score in predicting SAP,local complications,organ failure and mortality were 0.90 (95％ CI:0.86 ～ 0.93),0.82(95％ CI:0.76 ～ 0.89),0.93 (95％ CI:0.89 ～ 0.96),0.93 (95％ CI:0.87 ～ 0.98).There were no statistically significant differences in AUCs of the four prognostic parameters between BISAP and Ranson's score.The specificity,positive predictive value (PPV),and AUC of HAP score in predicting mild AP were 85％,95％ and 0.73 (95％ CI:0.67 ～ 0.79).The risk of dismal prognosis increased when both BISAP and HAP score were abnormal.Conclusions BISAP and Ranson's score have comparable ability in predicting prognosis of patients with AP.However,BISAP score is simpler.HAP score is a simple and accurate method for predicting prognosis of patients with mild AP.Combination of BISAP score with HAP score can better help predict the prognosis of AP patients. \u0000 \u0000Key words: \u0000Acute pancreatitis;  BISAP score ;  HAP score ;  Prognosis;  Scoring systems","PeriodicalId":63578,"journal":{"name":"中华胰腺病杂志","volume":"13 1","pages":"157-161"},"PeriodicalIF":0.0,"publicationDate":"2013-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of cobalt chloride mimetic hypoxia on the proliferation, apoptosis and migration of human pancreatic cancer cell line PANC1","authors":"Liang Zhu, Shengwei Xu, H. Pang, Hui-zhen Zhao, H. Qin, Pei-yuan Li, De-min Li, Qiu Zhao","doi":"10.3760/CMA.J.ISSN.1674-1935.2012.02.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-1935.2012.02.011","url":null,"abstract":"Objective To investigate the influence of cobalt chloride ( CoCl2 )-mimetic hypoxia on theproliferation,apoptosis and migration of human pancreatic cancer cell fine PANC1.Methods PANC1 cells were treated with 0(control),100,200,400,800 μmol/L CoCl2 respectively for 24 h.Real-time RT-PCR and Western blot were used to determine hypoxia induced factor ( HIF)-1o mRNA and protein expression respectively,and cell counting kit-8(CCK-8) assays,flow cytometry and cell scratch test were used to examine the proliferation,apoptosis and migration of PANC1 cells,respectively.Results In the control group and 100,200,400 and 800 μmol/L CoCl-2 groups,the expressions of HIF-1t mRNA were 1,1.08 ±0.12,1.12 ± 0.09,1.04±0.11,0.66 ±0.07,and the expressions of VEGF mRNA were 1,2.69±0.35,4.81 ±0.54,2.19 ± 0.21,0.79 ± 0.08,while the expressions of HIF-1 α protein were 0.23 ± 0.03,0.36 ± 0.04,1.15 ± 0.11,1.08 ± 0.09,0.44 ± 0.04; and the expressions of VEGF protein were 0.14 ± 0.02,0.12 ± 0.01,0.95 ±0.09,0.87 ±0.09,0.55 ±0.06; and cell viability rates were 100％,(98.43 ±2.88)％,(76.15 ± 0.70)％,(53.87 ±0.77)％,(35.23 ±0.67)％ ; while cell apoptotic rates were (5.2 ±1.12)％,(5.74 ± 1.07)％,(6.82 ± 1.85)％,(12.09 ±3.53)％,(31.88 ±6.95)％ ; the cell migration distance of PANC1 cells were (43.24 ±3.67)％,(59.46 ±5.39)％,(80.56 ±8.05)％,(63.89 ±5.96)％,(9.09 ± 1.59 ) ％.Compared with those of control group,the expressions of VEGF mRNA,VEGF and HIF-1 α protein,cell migration distance showed a two-way variation ( ascending first and descending later) (P ＜0.05 ),and the expression of HIF-1α mRNA and cell proliferation rate was decreased in a dose-dependent manner,while the cell apoptosis was increased in a dose-dependent manner.Conclusions CoCl2 significantly inhibits the proliferation and promotes apoptosis of PANC1 cells at certain level.CoCl2 has a two-way effect on the migration of PANC1 cells,and it may be related to the direct injury of high concentration of CoCl2 on cells. \u0000 \u0000Key words: \u0000Pancreatic neoplasms; Anoxia; Cobalt chloride; Cell proliferation; Apoptosis; Cell movement","PeriodicalId":63578,"journal":{"name":"中华胰腺病杂志","volume":"12 1","pages":"111-114"},"PeriodicalIF":0.0,"publicationDate":"2012-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of MEK signaling inhibitor on the growth of human pacreatic cancer cell lines and the expression of cell cyde associated genes","authors":"Xia Wang, Hui Wang, N. Jiang, S. Liang, Lu Wen, Xiao Zhang, Xiao-feng Zhang","doi":"10.3760/CMA.J.ISSN.1674-1935.2011.04.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-1935.2011.04.009","url":null,"abstract":"Objective To examine the effects of the MEK inhibitor on human pancreatic cancer cells, and to explore the molecular mechanisms. Methods Human pancreatic cancer cell lines CFPANC1, PANC1 and MiaPaCa2 were treated with MEK inhibitor PD98059 or DMSO, the sensitivity was analyzed by an MTT assay, and cell cycle distribution was evaluated by flow cytometry( FCM), The transcriptional level and protein expression of tumor suppressor genes were detected by real-time RT-PCR and western blot respectively. DNA methyltransferase (Dnmt)1, 3a and 3b were also assayed by western blot, The methylation status of the promoter of the p16INK4A gene was assayed by methylation-specific PCR (MSP). Results PD98059 inhibited to various degrees the growth of three pancreatic cancer cell lines, accompanied by G0-G1 cell cycle arrest. PD98059 up-regulated the expression of p16INK4a, p21WAF1, p27KIP1 mRNA, demethylated the hypermethylation status of p16INK4a gene promoter, and decreased Dnmtl and Dnmt3b in CFPANC1 and PANC1 cell lines. PD98059 only increased the expression of p27KIP1, while the changes of p16INK4a, p21WAF1 and Dnmt were less marked in MiaPaCa2 cell line. Conclusions MEK inhibitor PD98059 down-regulate the activation of Dnmt and up-regulate tumor supress genes, along with the inhibition of cell proliferation and cell cycle progression. \u0000 \u0000Key words: \u0000Pancreas;  Tunor cell line;  MEK inhibitor;  Cell cycle;  DNA methyltransferase; Tumor suppressor genes","PeriodicalId":63578,"journal":{"name":"中华胰腺病杂志","volume":"11 1","pages":"259-262"},"PeriodicalIF":0.0,"publicationDate":"2011-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of CD151 c-Met, integrin alpha 3, alpha 6 and the prognostic significance in pancreatic ductal adenocarcinoma","authors":"Zhikun Zhang, Z. Qiu, G. Zhu, Jun Liu, Ning-Ning Zhao, Chen Huang, Zheng-zhong Feng, Xiujuan Lu","doi":"10.3760/CMA.J.ISSN.1674-1935.2011.03.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-1935.2011.03.013","url":null,"abstract":"Objective To investigate the expression and prognostic significance of CD151, c-Met and integrin alpha 3, alpha6 in pancreatic ductal adenocarcinoma (PDAC). Methods The expression of CD151, c-Met and integrin alpha3, alpha6 in 71 patients with PDAC and 10 samples of normal pancreas tissues were detected by immunohistochemistry, and the relationship between the expression of CD151, c-Met and integrin alpha 3, alpha 6 and the clinicopathological features, prognosis of these patients was analyzed. Results The positive expression rates of CD151, c-Met and integrin alpha 3, alpha 6 in PDAC were 81.69% (58/71) , 69.01% (49/71), 69.01% (49/71) and 84.51% (60/71) , and there was no expression in normal pancreas tissues. The expressions of CD151, c-Met were significantly associated with TNM stage and lymph node metastasis (P < 0.05). The expression of CD151 was positively correlated with the expressions of c-Met and integrin alpha3, alpha6 (r =0.583, P =0.000, r = 0.457;P =0.000, r = 0.671 ;P =0.000). Univariate analysis suggested the expression of CD151, c-Met and integrin alpha3, alpha6 was associated with survival (P<0.05). Multivariate analysis suggested the expression of CD151, c-Met was the independent prognostic factor for post-operative survival. Conclusions CD151, c-Met and integrin alpha3, alpha6 play a role in the development, metastasis and prognosis of PDAC, and they might be new markers to predict biological behavior and the prognosis of PDAC patients. \u0000 \u0000Key words: \u0000Pancreas neoplasm;  CD151;  c-Met;  Integrin alpha3;  Integrin alpha6","PeriodicalId":63578,"journal":{"name":"中华胰腺病杂志","volume":"65 1","pages":"190-193"},"PeriodicalIF":0.0,"publicationDate":"2011-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis and treatment of postoperative gastroduodenal fistulas in patients with severe acute pancreatitis","authors":"S. Bei, L. Ji, J. Hongchi, L. Jun, Meng Qinghui, Liu Jie, Lin-feng Wu, Xiang-song Wu, Pan-quan Li","doi":"10.3760/CMA.J.ISSN.1674-1935.2008.05.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-1935.2008.05.012","url":null,"abstract":"Objective To investigate the etiologies, mechanisms, diagnosis and management of postoperative gastroduodenal fistulas in patients with severe acute pancreatitis (SAP). Methods The clinical data of 18 cases of postoperative gastroduodenal fistulas (PGF) in patients with SAP admitted in our hospital from Jan, 1996 to Dec, 2007 were analyzed retrospectively. Results Of 18 patients with SAP, 4 patients underwent cholecystectomy and common bile duct exploration, 4 patients underwent pancreatic debridement and open saucer-type drainage, 10 patients underwent pancreatic debridement and pancreas-bed mobilization, abdominal closed drainage. Duodenal fistula occurred in 12 patients (66.7%), gastric fistula occurred in 6 patients (33.3%). 14 patients (77.8%) developed gastroduodenal fistula within 3 weeks of surgery, and 4 patients developed gastroduodenal fistula after 3 weeks of surgery. 16 patients (88.9%) were cured with non-surgical management including drainage, antibiotics and abscess cavity douching. The average length of stay was 65 days. Two patients (11.1%) died of intra-abdominal hemorrhage and intra-abdominal infection complicated with abdominal compartment syndrome and multiple organ dysfunction syndromes, respectively. Conclusions SAP complicated with postoperative gaatroduodenal fistulas could be cured by appropriate non-surgical treatment. \u0000 \u0000Key words: \u0000Pancreatitis, acute necrotizing;  Postoperative complications;  Gastrodduodenal fistula","PeriodicalId":63578,"journal":{"name":"中华胰腺病杂志","volume":"8 1","pages":"322-323"},"PeriodicalIF":0.0,"publicationDate":"2008-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}