中华器官移植杂志最新文献

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Immune active effects of chemokine RANTES on human peripheral mononuclear cells 趋化因子RANTES对人外周血单核细胞的免疫活性作用

中华器官移植杂志 Pub Date : 2004-01-01 DOI: 10.1097/01.tp.0000331331.81663.48

Gu Xiao

{"title":"Immune active effects of chemokine RANTES on human peripheral mononuclear cells","authors":"Gu Xiao","doi":"10.1097/01.tp.0000331331.81663.48","DOIUrl":"https://doi.org/10.1097/01.tp.0000331331.81663.48","url":null,"abstract":"Objective To investigate the proliferation and phenotypes of peripheral mononuclear cells (PMNC) stimulated by recombinant human RANTES (rhRANTES) and the mechanisms involved.Methods PMNC was stimulated by various concentrations of rhRANTES and/or anti-CD3 mAb and intervened by PDTC and CTLA4Ig. Scintillation counter was used to count the cpm of proliferation cells and flow cytometry was used to detect the phenotypes of lymphocytes.Results rhRANTES was capable of directly stimulating purified human PMNC proliferation and two peaks occurred with rhRANTES concentration of 100 ng/ml and 5000 ng/ml respectively. The proliferation of PMNC stimulated by rhRNATES was significantly higher than that in the presence of anti-CD3mAb (P 0.05). The immune active effects of rhRANTES could be inhibited by PDTC or CTLA4Ig in a dose dependent manner. RANTES treatment of PMNC in vitro increased the level of cell surface CD25 and decreased the CCR5 expression. But it did not influence the ratio of CD4/CD8 and CD28 expression of lymphocytes.Conclusion RANTES is an important stimulatory mediator in human PMNC activation. This special signal works depending on the activation of IL-2 signal pathway, CD28 costimulatory pathway and nuclear factor-κB, but independent of CD3 activation.","PeriodicalId":60883,"journal":{"name":"中华器官移植杂志","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/01.tp.0000331331.81663.48","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"61657200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multivariate analysis of risk factors for clinically overt hemorrhagic cystitis after allogeneic hematopoietic stem cell transplantation 异基因造血干细胞移植后临床显性出血性膀胱炎危险因素的多因素分析

中华器官移植杂志 Pub Date : 1900-01-01 DOI: 10.1182/blood.v106.11.5295.5295

Z. Tao

{"title":"Multivariate analysis of risk factors for clinically overt hemorrhagic cystitis after allogeneic hematopoietic stem cell transplantation","authors":"Z. Tao","doi":"10.1182/blood.v106.11.5295.5295","DOIUrl":"https://doi.org/10.1182/blood.v106.11.5295.5295","url":null,"abstract":"Objective To analyze the risk factors of clinically overt hemorrhagic cystitis(HC)(grade ≥Ⅱ) in 114 patients undergoing allogeneic hematopoietic stem cell transplantation(allo-HSCT) to predict the occurrence of HC.Methods We retrospectively analyzed 29 cases of clinically overt HC from a series of 114 patients subject to allo-HSCT from April 1997 to December 2004.The time of follow-up began from the day of initiating conditioning to day 180 post-transplant.The 11 clinical parameters were selected for univariate analysis using a Cox regression: age,sex,underlying disease,conditioning regimen,disease status at transplant,aGVHD,donor type,use of ATG,GVHD prophylaxis,platelet and neutrophil engraftment.Factors that were significant at the 0.1 level on univariate analysis were evaluated by multivariate analysis using a Cox regression.The cumulative incidence of grade ≥Ⅱ HC within the day 180 after transplantation was calculated by the method of Kaplan and Meier.Results Twenty-nine out of 114 patients(26 %) developed HC with grade Ⅱ in 12/29 cases(41.4 %),grade Ⅲ in 11/29 cases(37.9 %) and grade Ⅳ in 6/29 cases(20.7 %).The following factors were associated with an increased risk of HC by univariate analysis: male gender(RR=2.885,P=0.021),younger than 26 years(RR=3.265,P=0.002),gradeⅢ～Ⅳ aGVHD(RR=4.039,P=0.002),unrelated donor(RR=4.347,P=0.000),intense GVHD prophylaxis(RR=2.218,P=0.045),advanced disease(RR=2.668,P=0.009).These risk factors were entered into a multivariate model.Only male gender(RR=2.993,95 % CI 1.218-7.358;P=0.017) and unrelated donor(RR=4.478,95 % CI 2.049-9.786;P=0.000) were identified as being significantly associated with the occurrence of HC.Conclusion In multivariate analysis,patients were at increased risk of HC if they were male or had received grafts from unrelated donors.","PeriodicalId":60883,"journal":{"name":"中华器官移植杂志","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65994824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0