Luz P Blanco, Melissa Plegue, Wai-Ping Fung-Leung, Joseph Holoshitz
{"title":"Gender-biased regulation of human IL-17-producing cells <i>in vitro</i> by peptides corresponding to distinct <i>HLA-DRB1</i> allele-coded sequences.","authors":"Luz P Blanco, Melissa Plegue, Wai-Ping Fung-Leung, Joseph Holoshitz","doi":"10.4236/jibtva.2013.23004","DOIUrl":"https://doi.org/10.4236/jibtva.2013.23004","url":null,"abstract":"<p><p>Susceptibility to rheumatoid arthritis (RA) is associated with <i>HLA-DRB1</i> alleles coding a 5-amino acid sequence motif called the shared epitope (SE). To explore the potential mechanisms that lead to RA susceptibility, we analyze the <i>in vitro</i> effect of peptides bearing different HLA-DR4 sequences on human peripheral blood-derived cells. Three 15-mer peptides were used: 65-79*0401 (an <i>HLA-DRB1*04:01</i>-coded sequence carrying the SE motif, QKRAA); 65-79*0402 (an <i>HLA-DRB1*04:02</i>-coded sequence carrying a SE-negative motif, DERAA); 65-79*0403 (an <i>HLA-DRB1*04:03</i>-coded sequence carrying a SE-negative motif, QRRAE). We found that CD4 TH17 cells are regulated by peptide treatment with gender bias. In male-derived T cells, all peptide treatments significantly reduced TH17 cell differentiation <i>in vitro</i> when compared to no peptide treatment, and to female samples. TH17 differentiation in samples not treated with peptides, either in the presence or absence of TH17-polarizing cytokines, was higher in males than in females; however, in unfractionated PBMC after treatment with TH17 polarizing cytokines, IL-17A positive cells were more abundant in females than in males. In addition, SE-positive females showed a significantly higher percentage of IL-17A-positive cells compared to SE-negative females. In conclusion, donor's SE status and gender may both influence TH17 immune polarization.</p>","PeriodicalId":60498,"journal":{"name":"基于免疫的治疗疫苗和抗菌期刊(英文)","volume":"2 3","pages":"29-38"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5523969/pdf/nihms882584.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35201815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}