Postepy Mikrobiologii最新文献

Microbial Biosurfactant Screening: Diversity in Assessment Methods 微生物生物表面活性剂筛选:评价方法的多样性

4区医学

Postepy Mikrobiologii Pub Date : 2023-11-01 DOI: 10.2478/am-2023-0013

Rafeya Sohail, Nazia Jamil

{"title":"Microbial Biosurfactant Screening: Diversity in Assessment Methods","authors":"Rafeya Sohail, Nazia Jamil","doi":"10.2478/am-2023-0013","DOIUrl":"https://doi.org/10.2478/am-2023-0013","url":null,"abstract":"Abstract Biosurfactants are a group of surface-active agents mainly produced by microorganisms and have a wide range of applications in industries as well as agriculture. Global usage of such biologically active compounds as detergents, bioremediation agents, cosmetic products is hugely dependent on their high production. New avenues of research have opened up due to this increased interest, most of which are focused on identifying specialized microorganisms capable of biosurfactant production. Screening methods for selection of biosurfactant producing bacteria are, therefore, of utmost importance in this endeavor. The aim of this review was to discuss various conventional and novel methods that can overcome the hurdles encountered in time effective, economical microbial biosurfactant screening. This review lists several important biosurfactant screening methods based on supernatant, biomass as well as culture media. New methods devised for biosurfactant screening in the last half decade such as surfactant dependent dye solubilization, metagenomic studies etc. are also explained in detail. A thorough grasp of both contemporary and anachronistic screening methodologies will not only lead to discoveries of novel native strains capable of biosurfactant production but also form the basis on which new screening methods can be devised.","PeriodicalId":54594,"journal":{"name":"Postepy Mikrobiologii","volume":"58 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135516039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Galleria Mellonella Larvae as an In vitro Model for Testing Microbial Pathogenicity Mellonella幼虫作为微生物致病性测试的体外模型

4区医学

Postepy Mikrobiologii Pub Date : 2023-11-01 DOI: 10.2478/am-2023-0015

Dawid Gniazdo, Wanesa Sasal, Aleksandra Omelaniuk, Weronika Brudz, Wiesław Kaca

引用次数: 0

Exploring Bacterial Diversity: How Far Have We Reached? 探索细菌多样性:我们已经走了多远?

4区医学

Postepy Mikrobiologii Pub Date : 2023-11-01 DOI: 10.2478/am-2023-0011

Himani Pandey, Devi Lal

{"title":"Exploring Bacterial Diversity: How Far Have We Reached?","authors":"Himani Pandey, Devi Lal","doi":"10.2478/am-2023-0011","DOIUrl":"https://doi.org/10.2478/am-2023-0011","url":null,"abstract":"Abstract Many methods have been developed for studying and comparing bacterial diversity. These methods suffer from a number of drawbacks. Culture-dependent methods have a drawback that only a small number of bacteria can be cultured. Although many modifications in the traditional cultivation approach have been made, such as the use of gellan instead of agar and high throughput dilution to extinction culturing, but a large fraction of microbes still remain uncultured. Culture-independent methods were developed to explore uncultured bacterial diversity but they have their own drawbacks. PCR-based methods have biases during DNA extraction and the removal of substances that may inhibit polymerase activity during PCR and digestion with restriction enzymes. “Omics” approach, i.e., metagenomics, metatranscriptomics, and metaproteomics, aim to link bacterial community structure with function. Different combinations of methods can be used to know more precisely about the bacterial diversity. To date, no known method can reveal the exact bacterial diversity of different environments. This lacuna needs to be filled and newer methods must be developed that can help in exploring the immense bacterial diversity created by nature.","PeriodicalId":54594,"journal":{"name":"Postepy Mikrobiologii","volume":"60 10","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135516026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stenotrophomonas maltophilia – Znaczenie Kliniczne, Leczenie Zakażeń 嗜麦芽血单胞菌 - 临床意义、感染治疗

4区医学

Postepy Mikrobiologii Pub Date : 2023-11-01 DOI: 10.2478/am-2023-0012

Joanna Nowicka, Adriana Janczura, Martyna Lelonkiewicz

引用次数: 0

Rola Wybranych Czynników Wirulencji w Zakażeniach Wywoływanych Przez Szczepy Enterococcus spp. 某些毒力因子在肠球菌属菌株引发的感染中的作用

4区医学

Postepy Mikrobiologii Pub Date : 2023-11-01 DOI: 10.2478/am-2023-0014

Zuzanna Kraszewska, Iwona Skuczyńska, Tomasz Bogiel, Eugenia Gospodarek-Komkowska

{"title":"Rola Wybranych Czynników Wirulencji w Zakażeniach Wywoływanych Przez Szczepy <i>Enterococcus</i> spp.","authors":"Zuzanna Kraszewska, Iwona Skuczyńska, Tomasz Bogiel, Eugenia Gospodarek-Komkowska","doi":"10.2478/am-2023-0014","DOIUrl":"https://doi.org/10.2478/am-2023-0014","url":null,"abstract":"Streszczenie Ziarenkowce z rodzaju Enterococcus spp. są składnikiem mikrobioty ludzi i zwierząt oraz powszechnie występują w środowisku naturalnym. Należą do patogenów warunkowo chorobotwórczych, mogących wywoływać zakażenia o różnej lokalizacji. Bakterie te rzadko wywołują zakażenia pozaszpitalne. Mimo, że do niedawna były uważane za drobnoustroje o niskim potencjale chorobotwórczym, stały się jednym z istotnych patogenów szpitalnych. Ich powszechne występowanie i zdolność do przeżycia w środowisku szpitalnym przyczyniają się do notowanej od kilku lat i nadal wzrastającej częstości ich izolacji, również z zakażeń inwazyjnych. Gatunki najczęściej izolowane z przypadków zakażeń to E. faecalis i E. faecium , stwarzające problemy terapeutyczne w związku z ich narastającą wielolekoopornością. Z powodu rosnącego znaczenia klinicznego, mechanizmów naturalnej i nabytej oporności na antybiotyki oraz potencjalnych czynników wirulencji, ziarenkowce z rodzaju Enterococcus stały się przedmiotem wielu badań. Celem pracy jest przedstawienie aktualnej wiedzy dotyczącej najistotniejszych czynników wirulencji mogących występować u bakterii z rodzaju Enterococcus , do których należą – antygen wydzielniczy SagA, białko EfaA, białko powierzchniowe Esp, białko wiążące kolagen Ace, cytolizyna, hialuronidaza, hemaglutynina, lipaza, proteaza serynowa, substancja agregująca, zewnątrzkomórkowe nadtlenki oraz żelatynaza.","PeriodicalId":54594,"journal":{"name":"Postepy Mikrobiologii","volume":"2 10","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135515392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0