FigsharePub Date : 1753-01-01DOI: 10.1021/acssynbio.0c00647.s001
Yongfei Liu (713467), Huiling Yuan (806135), Dongqin Ding (713286), Huina Dong (713466), Qinhong Wang (239716), Dawei Zhang (58801)
{"title":"Establishment of a Biosensor-based High-ThroughputnScreening Platform for Tryptophan Overproduction","authors":"Yongfei Liu (713467), Huiling Yuan (806135), Dongqin Ding (713286), Huina Dong (713466), Qinhong Wang (239716), Dawei Zhang (58801)","doi":"10.1021/acssynbio.0c00647.s001","DOIUrl":"https://doi.org/10.1021/acssynbio.0c00647.s001","url":null,"abstract":"With the flexibility to fold intoncomplex structures, RNA is well-suitednto act as a cellular sensor to recognize environmental fluctuationsnand respond to changes by regulating the corresponding genes. In thisnstudy, we established a high-throughput screening platform to screenntryptophan high-producing strains from a large repertoire of candidatenstrains. This platform consists of a tryptophan-specific aptamer-basednbiosensor and fluorescence-activated droplet sorting technology. Onenmutant strain, with a 165.9% increase in Trp titer compared with thenparental strain, was successfully screened from a random mutagenesisnlibrary. Sequencing results revealed that a total of 10 single-nucleotidenpolymorphisms were discovered in the genome of the mutant strain,namong which CRP(T29K) was proven to significantly increase Trp productionnthrough improving the strain’s tolerance of the harsh environmentnduring the stationary phase of the fermentation process. Our resultsnindicate that this strategy has great potential for improving thenproduction of other amino acids in <i>Escherichia coli</i>.","PeriodicalId":523786,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1753-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147921942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}