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Quantifying IntracellularnNanoparticle Distributionsnwith Three-Dimensional Super-Resolution Microscopy 三维超分辨率显微镜定量细胞内纳米颗粒分布

Figshare Pub Date : 2023-04-18 DOI: 10.1021/acsnano.2c12808.s002

Vinit Sheth (12089566), Xuxin Chen (457997), Evan M. Mettenbrink (12089575), Wen Yang (235745), Meredith A. Jones (15315046), Ons M’Saad (15315049), Abigail G. Thomas (13570186), Rylee S. Newport (15315052), Emmy Francek (15315055), Lin Wang (11986), Alex N. Frickenstein (12089572), Nathan D. Donahue (8473431), Alyssa Holden (12505334), Nathan F. Mjema (15315058), Dixy E. Green (2117737), Paul L. DeAngelis (2117743), Joerg Bewersdorf (667350), Stefan Wilhelm (95263)

{"title":"Quantifying IntracellularnNanoparticle Distributionsnwith Three-Dimensional Super-Resolution Microscopy","authors":"Vinit Sheth (12089566), Xuxin Chen (457997), Evan M. Mettenbrink (12089575), Wen Yang (235745), Meredith A. Jones (15315046), Ons M’Saad (15315049), Abigail G. Thomas (13570186), Rylee S. Newport (15315052), Emmy Francek (15315055), Lin Wang (11986), Alex N. Frickenstein (12089572), Nathan D. Donahue (8473431), Alyssa Holden (12505334), Nathan F. Mjema (15315058), Dixy E. Green (2117737), Paul L. DeAngelis (2117743), Joerg Bewersdorf (667350), Stefan Wilhelm (95263)","doi":"10.1021/acsnano.2c12808.s002","DOIUrl":"https://doi.org/10.1021/acsnano.2c12808.s002","url":null,"abstract":"Super-resolution microscopy can transform our understandingnofnnanoparticle–cell interactions. Here, we established a super-resolutionnimaging technology to visualize nanoparticle distributions insidenmammalian cells. The cells were exposed to metallic nanoparticlesnand then embedded within different swellable hydrogels to enable quantitativenthree-dimensional (3D) imaging approaching electron-microscopy-likenresolution using a standard light microscope. By exploiting the nanoparticles’nlight scattering properties, we demonstrated quantitative label-freenimaging of intracellular nanoparticles with ultrastructural context.nWe confirmed the compatibility of two expansion microscopy protocols,nprotein retention and pan-expansion microscopy, with nanoparticlenuptake studies. We validated relative differences between nanoparticlencellular accumulation for various surface modifications using massnspectrometry and determined the intracellular nanoparticle spatialndistribution in 3D for entire single cells. This super-resolutionnimaging platform technology may be broadly used to understand thennanoparticle intracellular fate in fundamental and applied studiesnto potentially inform the engineering of safer and more effectivennanomedicines.","PeriodicalId":523718,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147920704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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