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Targeted BiodegradablenNear-Infrared Fluorescent Nanoparticlesnfor Colorectal Cancer Imaging 靶向生物可降解的近红外荧光纳米颗粒用于结直肠癌成像

Figshare Pub Date : 1753-01-01 DOI: 10.1021/acsabm.4c00072.s001

Seock-Jin Chung (9570136), Kay Hadrick (18309783), Md Nafiujjaman (1918939), Ehsanul Hoque Apu (2813500), Meghan L. Hill (9570139), Md Nurunnabi (1766245), Christopher H. Contag (219505), Taeho Kim (1275954)

{"title":"Targeted BiodegradablenNear-Infrared Fluorescent Nanoparticlesnfor Colorectal Cancer Imaging","authors":"Seock-Jin Chung (9570136), Kay Hadrick (18309783), Md Nafiujjaman (1918939), Ehsanul Hoque Apu (2813500), Meghan L. Hill (9570139), Md Nurunnabi (1766245), Christopher H. Contag (219505), Taeho Kim (1275954)","doi":"10.1021/acsabm.4c00072.s001","DOIUrl":"https://doi.org/10.1021/acsabm.4c00072.s001","url":null,"abstract":"Colorectal cancer (CRC) is the third leading cause ofncancer deathnin the U.S., and early detection and diagnosis are essential for effectiventreatment. Current methods are inadequate for rapid detection of earlyndisease, revealing flat lesions, and delineating tumor margins withnaccuracy and molecular specificity. Fluorescence endoscopy can generatenwide field-of-view images enabling detection of CRC lesions and margins;nincreased signal intensity and improved signal-to-noise ratios cannincrease both speed and sensitivity of cancer detection. For thisnpurpose, we developed targeted near-infrared (NIR) fluorescent silicannanoparticles (FSNs). We tuned their size to 50–200 nm andnconjugated their surface with an antibody to carcinoembryonic antigenn(CEA) to prepare CEA-FSNs. The physicochemical properties and biodegradablenprofiles of CEA-FSN were characterized, and molecular targeting wasnverified in culture using HT29 (CEA positive) and HCT116 (CEA negative)ncells. CEA-FSNs bound to the HT29 cells to a greater extent than tonthe HCT116 cells, and smaller CEA-FSNs were internalized into HT29ncells more efficiently than larger CEA-FSNs. After intravenous administrationnof CEA-FSNs, a significantly greater signal was observed from thenCEA-positive HT29 than the CEA-negative HCT116 tumors in xenograftednmice. In F344-PIRC rats, polyps in the intestine were detected bynwhite-light endoscopy, and NIR fluorescent signals were found in thenexcised intestinal tissue after topical application of CEA-FSNs. Immunofluorescencenimaging of excised tissue sections demonstrated that the particlensignals coregistered with signals for both CRC and CEA. These resultsnindicate that CEA-FSNs have potential as a molecular imaging markernfor early diagnosis of CRC.","PeriodicalId":523701,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1753-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147920461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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